Desogestrel

Molecular formula: C22H30O Molecular weight: 310.5 CAS Registry No.: 54024-22-5

SAMPLE

Matrix: mucosal fluid Sample preparation: Extract 1 mL mucosal fluid twice with 5 mL diethyl ether, evaporate to dryness, reconstitute in 100 |JLL MeOH, inject an aliquot. (Aqueous layer can be incubated with p-glucuronidase/sulfatase at 37 for 3 h before extraction.)
HPLCVARIABLES

Guard column: present but not specified Column: 100 X 8 uBondapak C18 Mobile phase: Gradient. MeCN: water from 55:45 to 100:0 over 15 min, maintain at 100: 0 for 30 min Detector: UV 214 OTHER SUBSTANCES Extracted: metabolites
KEYWORDS

also for microsomal incubations (J. Steroid Biochem. 1990; 35; 281)
REFERENCE
Madden, S.; Back, D.J.; Martin, CA.; Orme, M.L. Metabolism of the contraceptive steroid desogestrel by the intestinal mucosa. Br.J.Clin.PharmacoL, 1989, 27, 295-299

SAMPLE Matrix: solutions

HPLCVARIABLES

Column: 200 X 4 5 |xm Nucleosil RP-8 Mobile phase: MeCN: MeOH: water 3:76:21 Flow rate: 1 Injection volume: 20 Detector: UV 205
CHROMATOGRAM

Retention time: 7
OTHER SUBSTANCES

Simultaneous: metabolites, ethinyl estradiol, 3-hydroxydesogestrel, 3-ketodesogestrel, 6ketoethinyl estradiol

REFERENCE
Smilde, A.K.; Bruins, C.H.R; Doornbos, D.A.; Vink, J. Optimization of the reversed-phase high-performance liquid chromatographic separation of synthetic estrogenic and progestogenic steroids using the multi-criteria decision making method. J.Chromatogr., 1987, 410, 1-12