Immunophenotypic and clinicopathologic studies on soft tissue tumors

Markku Miettinen, M.D.

Primary Research Area(s)

What are your expertise?
Histopathology of tumors Tissue immunohistochemistry

What technologies / approaches / methodologies do you utilize in your research area? Detailed histologic analysis Tissue immunohistochemistry DNA recovery from formalin-fixed and paraffin-embedded tissue PCR amplification to generate sequencing targets Sanger sequencing (technical procedure outsourced) Clinical correlation for tumor behavior Microphotography FISH (in collaboration)

Examples / Data / Results From Research Area

Analyzed succinate dedydrogenase deficient GISTs and renal and other carcinomas
Pathomechanisms of SDH-deficient GISTs, IGFR1R signaling

Analyzed KRAS mutations in GISTs Clinicopathologic analysis of myxohyaline tumors of distal extremities Analyzed tissue distribution of endothelial cell antigens
Claudin 5, Prox1, VEGFR2

Analyzed potential applications of GATA3 in surgical pathology Studied ERG-expression in epithelioid sarcoma and its biologic background

Research Implications
How does your research fit in the broader context? How does it fit into LP and broader areas?
Clinical research supports clinical practice

Data yields
diagnostic application of markers Increased understanding of specific tumor types

Future Direction
Where do you see your research going in the next 5-10 years? Continuing clinicopathologic analysis of soft tissue tumors and search for diagnostic and biologic markers of disease What are the current challenges & obstacles?
Lack of tissue availability, regulatory barriers

Collaborators
Who do you consider your primary collaborators & partnerships?
Jerzy Lasota Zengfeng Wang Martha Quezado Richard Lee David Kleiner Svetlana Pack Zied Abdullaev Mark Raffeld