Table 1 Formulation ingredients for preparing MSA core Tablets

Batch No.

Formulation Ingredients

Batch Size

MSA/tablet

Chitosan

Spray dried

MCC/tablet Colloidal

(mg)

(%w/v)

lactose /tablet

(% w/w)

silica

C1
C2

35
35

1
2

(% w/w)
10
10

_
_

(%w/w)
1
1

100
100

C3

35

10

100

C4

35

10

100

C5

35

10

100

C6

35

10

100

Table 2 Evaluation of core tablet

Batch
No

Hardness
(kg/cm2)

C1

Axial
Radial
Dia.
Dia.
(mm)
(mm)
2.020.03 4.010.03

% Weight
Variation

Friability
(%w/w)

4.40.38

Average
Weight
(mg)
44.721.10

C2

4.960.8

0.270.04

34.8730.12

91.2

951.1

2.040.06 4.020.05

4.20.41

44.591.92

4.951.1

0.350.05

33.9210.33

141.6

941.5

C3

2.030.05 4.030.08

4.90.26

44.891.47

4.981.2

0.320.08

34.7460.40

180.8

960.5

C4

2.050.02 4.020.04

4.30.48

43.942.01

4.880.7

0.400.01

33.8540.58

52.4

742.0

C5

2.010.01 4.040.02

4.10.22

44.241.84

4.911.6

0.420.07

33.9620.62

110.5

723.2

C6

2.020.04 4.050.01

4.00.56

44.672.42

4.971.9

0.450.09

34.2160.47

161.9

772.5

% Weight
Variation

% Weight
gain

5.070.2

10.661.02

Evaluation of coated tablets

Batch
Axial
Radial
Average
No
Diameter Diameter
Weight
(mm)
(mm)
(mg)
C3
2.090.05 4.460.35 49.771.10
(1:0.75
)
C3
(1:1)

2.140.07 4.680.29 50.271.65

5.020.5

11.710.09

C3
(1:1.25
)

2.070.09 4.540.56 49.262.05

4.920.4

9.461.66

Content
Disintegration
uniformity(mg)
time (mins)

%
yield

Table 3 Pharmacokinetic Parameters obtained after rat feeding of uncoated and coated
MSA tablet
Parameters

Core MSA tablet

Coated MSA tablet

Tlag

0h

4h

Cmax
Tmax
AUC

20.37g/ml
2h
32.2 g/ml/h

19.87g/ml
12h
151.4 g/ml/h

Ka

0.1053h-1

2.588 h-1

AUMC
MRT
Ke
Tlast

58.4 g/ml/h
1.81h
0.9164 h-1
24h

1688 g/ml/h
11.17h
1.932 h-1
24h

Table 4 Evaluation of colonic damage

Groups
I
II
III
IV
V
VI

TNBS treatment
(150mg/kg)
_
+
+
+
+
+

Dosage form
(MSA tablet)
_
_
Uncoated
Uncoated
Coated
Coated

Histopathological
Evaluation (Score)
0
5
4
3
2
0