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  • Enzyme Lab Analysis

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    1) As the concentration of the enzyme α-amylase increased from 1% to 10%, the reaction rate of hydrolyzing amylose increased linearly from 0.028 mg/sec to 0.167 mg/sec. 2) The reaction rate was highest at a pH of 7, reaching 0.2 mg/sec, and decreased at more extreme pH levels below 5 and above 9. 3) Both increasing enzyme concentration and maintaining a neutral pH of 7 optimized the reaction rate of α-amylase hydrolyzing amylose.

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    Data for enzyme lab.

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    1) As the concentration of the enzyme α-amylase increased from 1% to 10%, the reaction rate of hydrolyzing amylose increased linearly from 0.028 mg/sec to 0.167 mg/sec. 2) The reaction rate was highest at a pH of 7, reaching 0.2 mg/sec, and decreased at more extreme pH levels below 5 and above 9. 3) Both increasing enzyme concentration and maintaining a neutral pH of 7 optimized the reaction rate of α-amylase hydrolyzing amylose.

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    Download as XLSX, PDF, TXT or read online from Scribd
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    7 views3 pages

    Enzyme Lab Analysis

    Uploaded by

    s99happy
    1) As the concentration of the enzyme α-amylase increased from 1% to 10%, the reaction rate of hydrolyzing amylose increased linearly from 0.028 mg/sec to 0.167 mg/sec. 2) The reaction rate was highest at a pH of 7, reaching 0.2 mg/sec, and decreased at more extreme pH levels below 5 and above 9. 3) Both increasing enzyme concentration and maintaining a neutral pH of 7 optimized the reaction rate of α-amylase hydrolyzing amylose.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    Enzyme Concentration (%)

    Reaction Rate (mg/sec)


    1%
    2.50%
    5%
    10%
    2) Effects
    of pH on activity

    0.028
    0.048
    0.083
    0.167
    enzyme

    Figure
    of
    -Amylase in hydrolyzing amylose
    pH

    Reaction Rate (mg/sec)

    Reaction Rate (mg/sec)

    0.250

    5
    6
    7
    8
    9

    0.200
    0.150

    0.042
    0.125
    0.200
    0.100
    0.033

    0.100
    0.050
    0.000
    0

    5
    pH

    enzyme

    0.02777778
    0.04761905
    0.08333333
    0.16666667

    Figure 1) Effect of -Amylase concentration on the


    reaction rate of the hydrolysis of amylose
    0.180

    0.04166667
    0.125
    0.2
    0.1
    0.03333333

    Reaction Rate (mg/sec)

    0.160
    0.140
    0.120
    0.100
    0.080
    0.060
    0.040
    0.020
    0.000
    0%

    2%

    4%

    6%
    Enzyme Concentration (%)

    10

    8%

    centration on the
    is of amylose
    y = 1.5533x + 0.0095
    R = 0.9977

    10%

    12%

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