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New Improved CYCLE more Concentrated Technical Bulletin

1. Why did we reduce the number of bacterial strains? After two years of research, we have discovered that by reducing the number of strains to five (5 , the nitrifying power of Cycle increases in an exponential way. With some accessory bacterial strains removed from the formula, !room! is made, allowing bacteria res"onsible of the o#idation of ammonia and nitrites to be more stable, and to activate more rapidly . $f course, some s"ecies of heterotro"hic bacteria (Bacillus s". stay in the "roduct to %ee" the essential balance between autotro"hic and heterotro"hic bacteria.

&. 'ycle contains thousands of tiny "articles in sus"ension. (s this normal? )es, these little flocs are the visual "roof of the incredible increase of the nitrifying bacterial bio mass. The concentration of these beneficial bacteria is so high that it is now possible to see them without magnification. A single one of these little flocs observed with the microsco"e reveals millions of cyst-shaped bacteria, which need only to be added to an a*uarium to become active again.

+. Why the number of 5,, billions "er dose is not shown anymore on the label? Because of its new formula configuration, it is now extremely difficult to count the total bacterial cells in 'ycle. -ach little floc is "ac%ed with bacteria sealed together in a bio film and it is very difficult to se"arate them correctly, and thus achieve an accurate total cell count. .owever, the concentration of nitrifying bacteria is significantly increased in com"arison with the "revious version of 'ycle.

/. Why refrigeration after o"ening is not re*uired anymore? Because the cyst0sha"ed bacteria found in the micro flocs rapidly sin to the bottom of the container and are thus protected from oxygen which enters the bottle. The stabilised environment "revents any activity, even at room tem"erature. !nother added benefit to the consumer, the o"ened bottle of 'ycle can now be stored in a normal room environment without any threat to the "roduct integrity.

5. .ow "owerful is 'ycle? 'ycle is the most active product on the mar et. 1aboratory tests show its capacity to

oxidi"e more than #$ mg%L of ammonia per day &'easured as N( .

2. 3ew 'ycle starts is fully activated after )* hours instead 1// hours in the original formulation. 1,, mg41 of Ammonia (3.+03 is consumed in 1, days.

)van 5igu6re 1aboratory 7anager 1i*uid 8roducts 9ivision :olf '. .agen (nc.

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