Scribd Logo
Upload

Welcome to Scribd!

  • PCR

    Uploaded by

    Ho Zhen Meng
    9 views1 page

    Original Title

    12. PCR

    Copyright

    © Attribution Non-Commercial (BY-NC)

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    9 views1 page

    PCR

    Uploaded by

    Ho Zhen Meng

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    12. PCR - To amplify DNA.

    - A reaction mixture consisting of the DNA sample, free nucleotides, primers and DNA polymerase is set up. - The mixture is heated to 95oC to break the hydrogen bonds between DNA strands. - Then it is cooled down to 55oC so that primers can bind to the strand. - After that, it is heated to 72oC for DNA polymerase to work. - Free nucleotides are lined alongside each template strand via complementary base pairing. - Two new copies of DNA are formed. - The cycle continues.

    You might also like