B Chain

FVNQHLCGSHLVEALYLVCGERGFFYTPKT
B7-A7 B19-A20

GIVEQCCTSICSLYQLENYCN
A6-A11 A Chain

Fig: Amino Acid Sequence of Human Insulin

Trypsin Digest Fragments When Unfolded: F1:

F V N Q H L C G S H L V E A L Y L V C G E R = 2488.9

F2:

GIVEQCCTSICSLYQLENYCN

F3:

GFFYTPKT

V8 Protease Digest Fragments When Folded:

F1

= F V N Q H L C G S H L V E Q C C T S I C S L Y Q L E = 2954.42

F2

= A L Y L V C G E N Y C N = 1361.56

F3

= R G F F Y T P K T = 1117.29

F4

= G I V = 288.37