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Hemoglobin Hemoglobin?
the iron-containing oxygen-transport metalloprotein in the red blood cells of all vertebrates Hemoglobin in the blood carries oxygen and carbon dioxide Hemoglobin has a quaternary structure characteristic of many multi-subunit globular proteins Diagnostics Level Diseases High Hemoglobin level
MEN :13~18g/dL : 10~14g/Dl haemochromatosis
Deficiency
Anemia, Kidney fault
Hemoglobin
WOMEN :11.5~16.5g/dL
Infants
Measuring Principle
Cyanmethemoglobin method (Photometry)
Hb(Fe2+) Hb(Fe3+) :methemoglobin
KCN ,NaN3
K3Fe(CN)6, NaNO2
Principle
Cyanmethemoglobin (methemoglobinazide)
Hb(Fe3+ )
K4Fe2+CN6
Merit
Demerit
Optical interference Time consuming Large sample size Unstable cyanmethemoglobin toxic
Fe2+
RBC lysis hemolyzer Whole blood
e
Fe3+
Fe2+ Gold Electrode
Sales Points
Small Volume 1 ul
Specification