Proteins

Biochem I- Homework 9 Levels of Organization

Name : Cua ,Gwen ol!n "shle! #$

%" BC

&anuar! %', %'()

State whether the following statements are correct or incorrect. Justify/rationalize. ($ *econ ar! structural features of a +rotein are sta,ilize ,! covalent ,on s$ True. Because in both secondary structures , heli and ! "leated sheet assume hydrogen bonding and co#alent bonding. The heli bonds "arallel to the a is. $eanwhile ,! "lated sheets assume hydrogen bonding between its chains. %$ -earl! all +e+ti e grou+s in +roteins stu ie to ate have ,een foun to have the trans conformation$ %alse. &nly a few "e"tide grou" e ce"tions assume the trans conformation. 't is more stable com"ared to the cis conformation due to the the fact that cis conformation has steric interference between neighboring side chains. $oreo#er, only around ()* of +ro residues follow a cis "e"tide bond.

)$ #he rise +er amino aci resi ue in a segment of heli. is calle the +itch$ %alse. The "itch is the distance the heli rises along its a is "er turn in an heli . ,ather than a "itch it is called a -turn... /ecogniza,le com,inations of -helices an -stran s that a++ear in a num,er of ifferent +roteins are calle omains$ True. /omains is a grou" of one or a few "oly"e"tide segments of bout .)01)) residues that folds into a globular unit. 0$ #he h! ro+hilic si e-chains of amino aci resi ues normall! locate themselves towar the e.terior of glo,ular +roteins$ True. 2ydro"hilic, or water0soluble, amino acids ha#e ionized or "olar side chains. 1$ 2enaturation estro!s the ,iological activit! of a +rotein ,! ,reaking man! covalent ,on s in the +rotein$ %alse. /enaturation destroys the "e"tide bonds in the "rotein. 3$ #he 4uaternar! structure of +roteins is maintaine +re ominantl! ,! non-covalent interactions$ True . 3 multisubunit "rotein may consist of identical or non0identical "oly"e"tide chains. They contain closely "ac4ed non0"olar side chains , hydrogen bonds in#ol#ing the "oly"e"tide

bac4bones and their side chains , in some cases interchain disulfide bonds. 2owe#er , the subunit interfaces of "roteins that dissociate in #i#o ha#e lesser hydro"hobicites than the "ermanent interfaces. 3nswer the following "roblems. ($ " segment of a +rotein chain in the -helical conformation contains %' resi ues$ How long is this segment5 1)/5.. 6 7.8) residues "er 3 %$ How man! turns are in this helical segment of %' amino aci s ? 1)/ 7.9 6 5.55 : 9 Turns