Simultaneous Determination of Isoproturon and Carbendazim in Water Samples by Square Wave Anodic Stripping Voltammetry

Peeyanun Noyrod1, Suchada Chuanuwatanakul1,2*, Orawon Chailapakul1,2, Wanida Wonsawat3 Electrochemistry and Optical Spectroscopy Research Unit, Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand 2 Center of Excellent for Petroleum, Petrochemicals and Advanced Materials, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand 3 Department of Chemistry, Faculty of science and Technology, Suan Sunandha Rajabhat University, 1 UThong Nok Road, Dusit, Bangkok 10300, Thailand *E-mail: suchada.c@chula.ac.th ABSTRACT
1

An electrochemical method for simultaneous determination of two pesticides, namely isoproturon and carbendazim, in water samples are presented. The determinations are established by square wave anodic stripping voltammetry (SWASV) using graphene-based electrochemical sensor fabricated in our laboratory. Graphene offers excellent electrochemical properties because of its high electrical conductivity and large surface area. Therefore, it could effectively promote the electron transfer between the target molecule and the electrode surface. The experimental conditions were optimized. Under the optimum conditions, the analytical characteristics of the proposed method were evaluated. Linear calibration graphs were established in the range of 0.02 10.0 mg L-1 for isoproturon and 0.50 - 10.0 mg L-1 for carbendazim. The limits of detection were 0.02 mg L-1 for isoproturon and 0.11 mg L-1 for carbendazim. The relative standard deviations were 9.17 and 10.0 % for isoproturon and carbendazim, respectively. Water samples (rice-field water and Chao Praya river water) were filtered with 0.45 m filter membrane to remove particles, then simultaneous determination of isoproturon and carbendazim were performed using a standard addition method. The satisfactory recoveries of 106.9% for isoproturon and 85.46% for carbendazim in rice-field water and 92.06% for isoproturon and 84.41% for carbendazim in Chao Phraya river water, were obtained. Moreover, the results were comparable to those obtained from HPLC-UV method.
Keywords Pesticides; Isoproturon; Carbendazim; Anodic stripping voltammetry; Water samples