Serological and Molecular Methods in Virology Serological Methods 1.

Hemagglutination Assay (HA) method of quantification for viruses by hemagglutination relatively fast and easy and large amounts of samples use this method carried out in the presence of antibody performed in a microtiter plate

Molecular Methods 1. Nucleic Acid Probes segments of DNA or RNA that have been labeled with enzymes, antigenic substrates and radioisotopes

2. Polymerase Chain Reaction

2. Virus Neutralization Test used to quantify the titer of neutralizing antibody for a virus.

3. Complement Fixation Test used to discover if certain specific antigen or antibody is present in the serum of a patient used in microorganisms that are difficult to identify by culture methods.

4. Immunoprecipitation method in which a protein antigen that is precipitated out of solution specifically attaches to a specific protein isolating and concentrating a specific protein from a sample

5. ELISA (Enzyme Linked ImmunoSorbent Assay) used to detect whether an antibody or antigen is present in a sample

Types of ELISA Direct Indirect Competitive