DARE/ICAR ANNUAL REPORT 20032004 118

CAPTURE FISHERIES
Marine Sector
Estimation of marinefish production: Th e m a r i n e
fi sh l a n d i n g s i n I n d i a d u r i n g 2 0 0 2 - 2 0 0 3 wa s e st i m a t e d a t
2 . 6 4 m i l l i o n t o n n e s, wh i c h i s 0 . 3 1 4 m i l l i o n t o n n e s
( 1 3 . 5 %) h i g h e r t h a n t h a t o f t h e p r e vi o u s ye a r. Th e
i n c r e a se wa s p r i m a r i l y d u e t o e n h a n c e d c a t c h e s o f sh a r k s,
o i l sa r d i n e s, Bo m b a y d u c k , r i b b o n fi sh e s, c a r a n g i d s, se e r
fi sh e s, t u n a s, p e n a e i d p r a wn s a n d c e p h a l o p o d s. P e r c h e s
a n d n o n - p e n a e i d p r a wn s sh o we d a d e c r e a se i n l a n d i n g s.
Th e m e c h a n i z e d se c t o r a c c o u n t e d fo r 6 7 . 9 %, m o t o r i z e d
se c t o r 2 5 % a n d a r t i sa n a l se c t o r 7 . 1 % o f t h e p r o d u c t i o n .
Th e n o r t h - we st c o a st a c c o u n t e d fo r 0 . 9 0 8 m i l l i o n t o n n e s,
fo l l o we d b y so u t h - we st c o a st 0 . 8 6 m i l l i o n t o n n e s, so u t h -
e a st 0 . 6 1 1 m i l l i o n t o n n e s a n d n o r t h - e a st 0 . 2 2 7 m i l l i o n
t o n n e s. Mo n i t o r i n g o f e n vi r o n m e n t a l c h a r a c t e r i st i c s o f
c o a st a l wa t e r wa s c o n t i n u e d a l l t h r o u g h t h e ye a r.
I nland Sector
Application of an index of biological integrity (IBI)
in river Hooghly: A m u l t i m e t r i c i n d e x o f fi sh a sse m b l a g e s
i n t e g r i t y wa s d e ve l o p e d fo r fi sh sp e c i e s i n r i ve r Ho o g h l y
a n d t h e e ffe c t s o f m u n i c i p a l a n d i n d u st r i a l e ffl u e n t s a n d
p h ysi c a l c o n d i t i o n s o n t h o se i n d i c e s we r e e va l u a t e d . Th e
Bi o l o g i c a l i n t e g r i t y o f fi sh a sse m b l a g e s fr o m t h e si t e
Ho o g h l y g h a t d o wn st r e a m o f r i ve r Ho o g h l y i s i m p a i r e d .
Ecological status of Hooghly EstuarineSystem:
Ec o l o g y a n d fi sh e r i e s o f Ho o g h l y a n d Ma n d o vi - Zu r a i
e st u a r i n e syst e m s a n d p r o d u c t i o n p o t e n t i a l o f e st u a r i n e
we t l a n d s we r e e va l u a t e d . Ec o l o g i c a l st u d i e s r e ve a l e d h i g h
p r o d u c t i o n p o t e n t i a l s r e fl e c t i n g t o t a l fi sh yi e l d o f 6 2 , 5 5 4 . 7
t o n n e s. Hi l sa c o n t i n u e d t o b e a m a j o r c o m p o n e n t
c o n t r i b u t i n g 1 0 . 4 % o f t o t a l yi e l d fr o m Ho o g h l y e st u a r y.
P o p u l a t i o n o f Hi l sa i s d e c l i n i n g a t a fa st r a t e d u e t o
i n d i sc r i m i n a t e e x p l o i t a t i o n o f yo u n g Hi l sa b y d r i ft g i l l
n e t .
Inventory and mapping of inland water bodies of
Bihar: Ge o g r a p h i c a l I n fo r m a t i o n Syst e m ( GI S) wa s
d e ve l o p e d o n wa t e r b o d i e s fo r e i g h t d i st r i c t s o f Bi h a r. Th e
a r e a wa s e st i m a t e d u t i l i z i n g LI SS- I I I d a t a o f sa t e l l i t e I RS-
1 D. Di g i t a l b a se m a p wa s a l so p r e p a r e d fo r si x d i st r i c t s o f
Ra j a s t h a n .
CULTURE FISHERIES
Freshwater Aquaculture
Hatchery seed production of freshwater Prawn in
inland salinewater: Th e g i a n t p r a wn p o p u l a r l y k n o wn
a s Sc a m p i i s m i g r a t o r y a n d c o m p l e t e s i t s l i fe c yc l e i n
b o t h fr e sh a n d c o a st a l se a wa t e r. Se e d p r o d u c t i o n wa s d o n e
su c c e ssfu l l y i n fr e sh wa t e r p r a wn ( Macrobrachium
rosenbergii) b y u si n g u n d e r g o u n d sa l i n e wa t e r wi t h
n e c e ssa r y i o n i c a m e n d m e n t s a t Ro h t a k , Ha r ya n a . Ha r ya n a ,
Ra j a st h a n , P u n j a b a n d Ut t a r P r a d e sh h a ve l a r g e a r e a s o f
i n l a n d sa l i n e g r o u n d wa t e r r e se r ve s a n d h e n c e t h e p r e se n t
p r a c t i c e i s e x p e c t e d t o p r o vi d e b o o st t o p r a wn c u l t u r e
p r o g r a m m e s o f l a n d - l o c k e d st a t e s. Macrobrachium
rosenbergii c u l t u r e wa s i n i t i a t e d i n sa l i n e a ffe c t e d wa t e r s
o f P u n j a b a n d Ha r ya n a . P r a wn p r o d u c t i o n i n t h e r a n g e o f
8 7 2 t o 2 , 2 8 5 k g / h a wa s a c h i e ve d i n c u l t u r e p o n d s o f
c u l t u r e p e r i o d o f 6 - 7 m o n t h s. Effo r t s we r e a l so m a d e t o
t r a n sfe r t h i s t e c h n o l o g y t o Ut t a r P r a d e sh .
Monosex cultureof giant freshwater prawn: Mo n o se x
c u l t u r e o f M. rosenbergii c o n d u c t e d i n o u t d o o r c e m e n t
Fi sh Producti on and Processi ng
Morino isb lonoinqs imrovoo Ey 13.5 ovor rovious yoor
Eocls o municiol ono inouslriol oluonls on inoox o
Eioloqicol inloqrily ovoluoloo
Hilso oulolion ooclininq ouo lo inoiscriminolo oxloilolion
o younq bilso Ey oril qill nol
Diqilol Eoso mo oovolooo on wolor Eooios o somo
oislricls o 8ibor ono Ro|oslbon
Holcbory sooo roouclion o rosbwolor rown oxocloo lo
Eoosl rown culluro in lono-lockoo slolos
SuE-ooulls ono inqorlinqs o Foo||os o|cho||os woro
culluroo on orliiciol ooo
/ nolurol loko oovolooo os consorvolion silo or To
o|||oo
Molurolion orioo o oxolic cors sborlonoo ol biqb olliluoos
Kil oovolooo or ooloclion o wbilo sol virus
Cruoo oxlrocl o /|os oosson|o| ono Cs|ooqooo|oos
n||||o|s sbowoo boomolylic ono oooomolic oclivily
lmmuno inoox o liqor sbrim oovolooo lo imrovo
monoqomonl o Eroooslock
8roooslock o ornomonlol socios oovolooo
Fisbinq vossol wilb uol monilorinq oovolooo
Nolurol qonolic voriolion sluoioo in imorlonl isb socios
\ioElo roqony rooucoo usinq rozon-lbowoo sorms
Polymoroso cboin rooclion molboo usoo lo oolocl oxolic
olboqons
FISH PRODUCTION AND PROCESSING 119
c i st e r n s a t t wo st o c k i n g d e n si t i e s r e ve a l e d h i g h e r g r o wt h
r a t e s i n a l l - m a l e p o p u l a t i o n a s c o m p a r e d t o a l l - fe m a l e
p o p u l a t i o n r e a r e d u n d e r si m i l a r r e a r i n g c o n d i t i o n a n d
st o c k i n g d e n si t i e s. Fu r t h e r, t h e m a l e p o p u l a t i o n a l so
yi e l d e d h i g h e r p r o p o r t i o n o f m a r k e t a b l e si z e o f t h e p r a wn .
Breeding of peninsular carps: Se ve n se t s o f Labeo
fimbriatus we r e b r e d su c c e ssfu l l y u si n g a p o r t a b l e
h a t c h e r y a t Ba n g a l o r e . Mo r e t h a n 0 . 1 m i l l i o n sp a wn we r e
o b t a i n e d . Su b - a d u l t s a n d fi n g e r l i n g s o f Puntius
pulchellus we r e c o l l e c t e d fr o m t h e We st e r n Gh a t s a n d a r e
b e i n g c u l t u r e d u n d e r c a p t i vi t y o n a r t i fi c i a l fe e d .
I n vi t r o cultureof freshwater pearl mussel: P r i m a r y
in vitroc e l l c u l t u r e o f n a c r e se c r e t i n g p a l l i a l m a n t l e
e p i t h e l i a l t i ssu e e x p l a n t s o f fr e sh wa t e r p e a r l m u sse l
( Lamellidens marginalis) wa s c a r r i e d o u t su c c e ssfu l l y.
COLDWATER FISHERIES
Mahseer conservation initiativein Kumaon: A
n a t u r a l l a k e Sh ya m l a t a l i n Ku m a o n r e g i o n wa s d e ve l o p e d
a s a c o n se r va t i o n si t e fo r t h e t h r e a t e n e d m a h se e r sp e c i e s
Tor putitora. Ma h se e r fi n g e r l i n g s st o c k e d fo r fi r st t i m e
h a ve e st a b l i sh e d t h e m se l ve s we l l i n t h e l a k e wi t h 9 8 %
r e t u r n i n e x p e r i m e n t a l n e t t i n g . Th e m a x i m u m si z e o f
m a h se e r c a u g h t d u r i n g t h e t r i a l s i n g i l l n e t o p e r a t i o n s
wa s 7 0 0 g i n we i g h t a n d 2 6 0 m m i n l e n g t h . Wi t h t h e
e st a b l i sh m e n t o f m a h se e r st o c k s i n t h e l a k e , t h e
a va i l a b i l i t y o f m a t u r e sp a wn e r s fo r a r t i fi c i a l p r o p a g a t i o n
wi l l b e e n su r e d , o p e n i n g a n a d d i t i o n a l r e so u r c e fo r
c o n se r va t i o n a n d r e vi va l o f e n d a n g e r e d m a h se e r i n
Ku m a o n r e g i o n . Th i s wi l l a l so p r o m o t e a n g l i n g t o u r i sm
i n Sh ya m l a t a l l a k e a r e a .
Advanced maturation and breeding of exotic carps
at high altitudes: Wa t e r t e m p e r a t u r e wa s t h e l i m i t i n g
fa c t o r fo r m a t u r a t i o n a n d e m b r yo n i c d e ve l o p m e n t o f g r a ss
c a r p a n d si l ve r c a r p a t h i g h a l t i t u d e s. Th e e x p e r i m e n t s
Exorimonlol isbinq in Sbyomlolol loko in Kumoon
Mobsoor borvosloo rom culluroo onos
5uccess in incuboling ond reoring ol
roinbow lroul ol Bhimlol
For lbo irsl limo, oyoo ovo o roinEow lroul, Cocoh,ochos
n,||ss woro incuEoloo unoor wormor conoilions ol 8bimlol.
Holcbinq wos succossul ono inqorlinqs woro rooucoo wilbin
7C ooys o roorinq, wbilo loElo sizo wos olloinoo |usl wilbin ono
yoor o roorinq. Tbo lroul is normolly lronsorloo ol oyoo-ovo
sloqo Eul or lbo irsl limo CC inqorlinqs o roinEow lroul
bovinq o woiqbl ronqo o 3.45-5.Cq woro succossully
lronsorloo 2CC km
wilb no morlolily. Tbis
invosliqolion oons
u lbo ossiEilily o
roisinq loElo lroul ol
lowor olliluoo ono
lronsorlinq lbo
inqorlinqs Ey rooo in
bills.
Eqq slriinq rom omolo roinEow lroul
5UCCE55 5TOkY
Exolic corp lorming in mid-hills
Proviously slonooroizoo oxolic cor orminq locbnoloqy or
mio-bills, involvinq lbroo socios comEinolion o qross cor,
silvor cor ono common cor wos succossully oomonslroloo in
24 ormors onos siluoloo ol oioronl olliluoos in lwo oislricls
o lloroncbol. Cno o lbo isb ormors rom Toli villoqo
ocbiovoo biqbosl oslimoloo isb roouclion o [42 kq/bo/
yoor} Ey borvoslinq 12 kq isb rom 3CC m
2
ono, wilb on
oooilionol incomo o Rs 1134C rom o unil wolor oroo o 3CC
m
2
. Tbis sulomonls lbo incomo rom smoll orm boloinqs, in
wbicb corools, voqoloElos, ono ruils oro oovolooo. Tbis
inloqrolion o cro ono isb mokos bill orminq romunorolivo
ono susloinoElo. /ll lbo onos in lwo oislricls unoor lbis
roqrommo roqisloroo on oslimoloo ovoroqo isb roouclion o
35C8 kq/bo/yoor wilb ocluol borvosl in lbo ronqo o C.12 lo
C.3 kq/ m
2
/yoor in onos ronqinq in sizo Eolwoon 125 - 5CC
m
2
. Tbo roqrommo molivoloo moro ormors osociolly in
oislricls o Cbomowol ono Noinilol lo loko u isb culluro. /
sol-bol qrou bos Eoon orqonizoo in lbo villoqo Toli lo
lronsor lbis locbnoloqy lo ormors rom olbor oo|oininq villoqos
in lbo roqion.
DARE/ICAR ANNUAL REPORT 20032004 120
c o n d u c t e d r e ve a l e d t h a t t h e m a t u r i t y i n d u c i n g h o r m o n e
t r e a t m e n t wi t h HCG @ 2 5 0 - 3 0 0 I U a n d p i t u i t a r y e x t r a c t
p l u s o va p r i m i n 3 : 1 r a t i o @ 3 m l / k g c o u p l e d wi t h
i n su l a t i o n a g a i n st l o w t e m p e r a t u r e b y p o l yh o u se c o ve r i n g
o f p o n d s, wa s e ffe c t i ve i n a d va n c i n g m a t u r a t i o n p e r i o d b y
1 - 2 ye a r s. He n c e , b y a p p l i c a t i o n o f m a t u r i t y i n d u c i n g
h o r m o n e s a n d r a i si n g wa t e r t e m p e r a t u r e t h r o u g h
p o l yh o u se i n su l a t i o n a t h i g h a l t i t u d e r e g i o n s, t h e
m a t u r a t i o n p e r i o d o f t h e se sp e c i e s c a n b e sh o r t e n e d .
BRACKISHWATER AQUACULTURE
Demonstration of shrimp feed technology to the
coastal farmers: Sh r i m p fe e d d e ve l o p e d b y t h e CI BA wa s
su c c e ssfu l l y t e st e d i n a fa r m e r s p o n d a t Ka l p a k k a m , n e a r
Ch e n n a i . Th e 0 . 5 2 h a p o n d wa s st o c k e d wi t h t i g e r sh r i m p
Penaeus monodon se e d a n d t h e fa r m e r u se d CI BA sh r i m p
fe e d d u r i n g t h e c u l t u r e . Aft e r 1 3 7 d a ys o f c u l t u r e , t h e
fa r m e r h a r ve st e d 1 , 6 6 5 k g o f sh r i m p a n d o b t a i n e d a
p r o d u c t i o n o f 3 , 3 3 0 k g / h a .
Latex agglutination kit for detection of whitespot
virus: La t e x a g g l u t i n a t i o n k i t fo r d e t e c t i o n o f wh i t e sp o t
vi r u s i n sh r i m p s wa s d e ve l o p e d . Th i s i s a n o n - fa r m t e st
wi t h t h e a i d o f t h e r a p i d d i a g n o st i c k i t wh i c h c a n b e
c o m p l e t e d wi t h i n 3 - 4 m i n . La t e x a g g l u t i n a t i o n k i t a l o n g
wi t h t h e ELI SA a n d d o t - ELI SA k i t s fo r t h e d e t e c t i o n o f
p a t h o g e n i c b a c t e r i a , vi z . Pseudomonas fluorescens,
Aeromonas hydrophila, Vibrio alginolyticus a n d
Edwardsiella tarda o f fi sh , we r e c o m m e r c i a l i z e d .
Wound healing, antineoplastic and antioxidant
compounds from two marinecrinotoxic fishes: Cr u d e
m u c u s e x t r a c t o f Arius dussumieri a n d Osteogeneiosus
militaris e x h i b i t e d t o x i c i t y wh e n t e st e d o n m i c e . Th e
c r u d e m u c u s e x t r a c t o f A. dussumeiri sh o we d t h e h i g h e st
t o x i c i t y @ o f 0 . 3 0 m l a n d t h e m i c e d i e d i n 5 0 m i n u t e s,
wh e r e a s i n O. militaris, t h e t o x i c d o se wa s 0 . 5 0 m l ,
wh i c h c a u se d m o r t a l i t y i n 8 0 m i n u t e s. Ha e m o l yt i c a ssa y
c o n d u c t e d a g a i n st c h i c k e n e r yt h r o c yt e s sh o we d t h a t t h e
c r u d e m u c u s e x t r a c t s a n d p a r t i a l l y p u r i fi e d fr a c t i o n s o f
b o t h t h e fi sh e s h a s h a e m o l yt i c a s we l l a s o e d e m a t i c
a c t i vi t y.
Immuneindex of tiger shrimp: I m m u n e i n d e x wa s
d e ve l o p e d t o a sse ss t h e h e a l t h st a t u s o f t i g e r sh r i m p ,
Penaeus monodon, b a se d o n t h e c h a r a c t e r i st i c s o f i t s
h a e m o l ym p h . Th e r e wa s c o n si d e r a b l e va r i a t i o n i n t h e
h a e m o c yt e c o u n t o f t h e n o r m a l sh r i m p s. Du r i n g wh i t e
sp o t syn d r o m e vi r u s ( WSSV) i n fe c t i o n , t h e h a e m o c yt e
c o u n t s d r o p p e d d r a st i c a l l y. Th e se o b se r va t i o n s h a ve
p r a c t i c a l i m p l i c a t i o n i n t h e m a i n t e n a n c e o f b r o o d st o c k .
Mariculture
Maturation, spawning and larval rearing of
groupers: I n d u c e d m a t u r a t i o n o f g r o u p e r s b y h o r m o n e
i n j e c t i o n u si n g LHRh a wa s c o n d u c t e d a t Ma n d a p a m .
Na t u r a l sp a wn i n g o f Epinephelus tauvina a n d E.
polyphekadion wa s o b se r ve d u n d e r c a p t i ve c o n d i t i o n s. I n
E.tauvina, t wo sp a wn i n g s o c c u r r e d r e su l t i n g i n 3 . 9
m i l l i o n e g g s, a n d 2 . 7 m i l l i o n l a r va e we r e p r o d u c e d fr o m
e g g s .
Development of broodstock of ornamental fish:
Br o o d st o c k o f fi ve sp e c i e s o f d a m se l fi sh e s, fi l a m e n t o u s
t a i l b l a c k d a m se l Neopomacentrus cyanomos, ye l l o w t a i l
d a m se l , N. nemurus, b l u e d a m se l , Pomacentrus
caeruleus, p e a c o c k d a m se l , P. paco a n d I n d i a n d a sc yl l u s
Dascyllus carneus we r e su c c e ssfu l l y d e ve l o p e d .
FISH HARVEST AND PROCESSING
TECHNOLOGY
A n o ve l d e si g n o f a 1 5 . 5 m OAL, 1 2 5 h p st e e l fi sh i n g
ve sse l ( CI FTECH 1 ) wi t h sp l i t l e ve l d e c k c o n st r u c t e d fo r
t r a wl i n g , g i l l n e t t i n g a n d l i n i n g wa s d e ve l o p e d a n d a
ve sse l c o n st r u c t e d . A fu e l c o n su m p t i o n m o n i t o r fo r
m e a su r e m e n t o f fu e l c o n su m p t i o n r a t e , i n fl o w a n d r e t u r n
fl o w wa s d e ve l o p e d .
A si m p l e t e c h n i q u e wa s d e ve l o p e d t o r e d u c e t h e
c h e m i c a l h a z a r d o f b e n z o p yr e n e i n h o t sm o k e d fi sh e r y
p r o d u c t s, p a r t i c u l a r l y t u n a . A p r o d u c t wi t h c a l c i u m a n d
p h o sp h a t e i n 2 : 1 p r o p o r t i o n a n i d e a l r e q u i r e m e n t fo r
h u m a n c o n su m p t i o n wa s o b t a i n e d wh e n t u n a b o n e s
we r e h yd r o l yse d e n z ym a t i c a l l y.
Oysler lorming in Kerolo esluories
Tbo locbnoloqy or lbo roouclion o qourmol oyslors wos
oooloo Ey moro lbon 25C ormors in osluorino oroos o
soulborn lnoio covorinq on oroo o 2.2 bo. Tbo lolol roouclion
ourinq 2CC2 wos 35C lonnos sboll-on, yioloinq 35CC kq mool,
worlb Rs 21CCCC/- rom monlbs o orminq. Cyslor orminq
bos Eoon oovolooo os o communily-Eosoo roqrommo in
Korolo.
/ uol oicionl ooo soo mulliuroso 15.5 m. C/L isbinq vossol
ClFTECH -1" oovolooo Ey ClFT, Kocbi
FISH PRODUCTION AND PROCESSING 121
FISH GENETICS RESOURCES
Molecular markers and natural genetic variation in
important fish species
Co m b i n e d g e n o t yp i c d a t a o f 1 0 m i c r o sa t e l l i t e l o c i o f
Catla catla we r e c o l l e c t e d fr o m r i ve r s Su t l a j , Ga n g a ,
Gh a g r a , Ra p t i , Bh a g i r a t h i , Br a h m a p u t r a , Ma h a n a d i a n d
Go d a va r i , a n d a n a l yz e d . Ca t l a sa m p l e s a n a l yse d e x h i b i t e d
si g n i fi c a n t va r i a t i o n a t m i c r o sa t e l l i t e l o c i . Th e r e su l t s
r e ve a l e d t h a t c a t l a i n d i ffe r e n t r i ve r s i n I n d i a h a s d i st i n c t
p o p u l a t i o n su b st r u c t u r e .
m a r k e r s we r e d e ve l o p e d t o d e t e c t sp e c i e s- sp e c i fi c d i ffe r e n c e
b e t we e n C. chitala a n d Notopterus notopterus.
Karyological characterisation of fish species endemic
to Western Ghats: Ge n e t i c c h a r a c t e r i z a t i o n o f Labeo
dussumeri, Puntius filamentosus, Puntius denisonii,
Puntius sarana subnasutus, Horabagrus brachysoma,
Horabagrus nigricollaris, Pristolepis marginata a n d
Gerrus filamentosus e n d e m i c t o We st e r n Gh a t s, wa s d o n e .
Baselinefrequency of micronuclei in Channa
punctatus a n d Mystus vittatus: Th e fr e q u e n c y o f
m i c r o n u c l e i wa s 0 . 0 6 0 7 8 % i n fr e sh l y c o l l e c t e d Channa
punctatus sp e c i m e n s, h o we ve r, a ft e r a c c l i m a t i z a t i o n t h e
fr e q u e n c y o f m i c r o n u c l e i r e d u c e d t o 0 . 0 2 5 9 7 %. Si m i l a r l y
fr e q u e n c y o f m i c r o n u c l e i i n u n a c c l i m a t i z e d Mystus
vittatus wa s 0 . 0 5 6 7 4 % a n d a ft e r a c c l i m a t i z a t i o n i t wa s
0 . 0 3 2 7 %. Th e se fi n d i n g s i n d i c a t e d t h a t fi sh e s o f Go m t i
r i ve r we r e b e i n g e x p o se d t o g e n o t o x i c a n t s p r e se n t i n t h e
p o l l u t e d r i ve r wa t e r.
Development of sperm cryopreservation protocols for
Ompok malabaricus: Su c c e ssfu l c r yo p r e se r va t i o n o f
Ompok malabaricus sp e r m a t o z o a wi t h M- HBSS e x t e n d e r
a n d DMSO c r yo p r o t e c t a n t wa s a c h i e ve d . Vi a b l e p r o g e n y
we r e p r o d u c e d u si n g fr o z e n - t h a we d sp e r m s.
Diagnostic capability to detect exotic pathogens for
Collle/poullry leed lrom lishery wosle
/ molboo or roorolion o qooo guolily colllo/oullry ooo
sulomonl Ey onsilinq isbory woslo wos oulorisoo. Tbis bos
solvoo lbo onvironmonlol roElom cousoo Ey lbo oocoyinq
woslo Ey convorlinq il inlo o usoul rooucl. / molboo wos
oovolooo or lbo isololion o cbonoroilin sulbolo, o mooicinol
rooucl usoo in lbo lroolmonl o orlbrilis olionls, rom sbork
Eonos ono sbork corliloqo. Tbo rocoss ono roouclion
oromolors or ormonloo isb mool wos slonooroizoo. Tbo
rooucl bos ovoroqo moisluro conlonl o 22, roloin 4, ol
1.8 ono minorol conlonl 2.
Socios-sociic R/PD roilo o Ch||o|o ch||o|o ono No|o|oos oo|o|oos
oEloinoo lbrouqb CP83 rimor.
Themicrosatelliteprimers for yellow catfish
Horabagrus brachysoma, a n e n d a n g e r e d fi sh o f t h e
We st e r n Gh a t s, we r e d e ve l o p e d t h r o u g h c r o ss sp e c i e s
a m p l i fi c a t i o n o f h e t e r o l o g o u s p r i m e r s. Th e i d e n t i fi e d
m i c r o sa t e l l i t e DNA m a r k e r s e x h i b i t e d si g n i fi c a n t p o t e n t i a l
t o d e t e r m i n e fi n e sc a l e p o p u l a t i o n st r u c t u r e o f t h e sp e c i e s
a c r o ss i t s n a t u r a l r a n g e o f d i st r i b u t i o n . Mi c r o sa t e l l i t e DNA
m a r k e r s st u d i e s r e ve a l e d g e n e t i c va r i a t i o n s a t RAP D l o c i
e x h i b i t i n g si g n i fi c a n t d i ve r g e n c e i n Chitala chitala
sp e c i e s c o l l e c t e d fr o m d i ffe r e n t l o c a t i o n s. Di a g n o st i c RAP D
Conolic voriolion in |ooboqos boch,sono ol lwo microsolollilo loci omliioo
lbrouqb Scloror7 rimor
Koryolyo o Foo||os ooo|soo|| sbowinq NCRs
DARE/ICAR ANNUAL REPORT 20032004 122
fish quarantine: Sp e c i fi c d e t e c t i o n o f Yersinia ruckeri
a n d Aeromonas salmonicida wa s c a r r i e d o u t t h r o u g h
p o l ym e r a se c h a i n r e a c t i o n . Th e d e t e c t i o n o f vi r a l
h a e m o r r h a g i c se p t i c a e m i a ( VHS) wa s a l so c a r r i e d o u t b y
r e ve r se t r a n sc r i p t i o n p o l ym e r a se c h a i n r e a c t i o n yi e l d i n g .
Th e r a p i d d i a g n o si s o f e x o t i c p a t h o g e n s wi l l b e u se d fo r
sc r e e n i n g t h e i m p o r t e d fi sh a n d fi sh p r o d u c t s, a n d t o
p r e ve n t t h e e n t r y o f e x o t i c p a t h o g e n s i n t h e c o u n t r y.
HUMAN RESOURCE DEVELOPMENT
At t h e CI FE, Mu m b a i , t r a i n i n g p r o g r a m m e s o n Aq u a t i c
An i m a l To x i n s a n d P h a r m a c o l o g i c a l Bi o r e so u r c e s we r e
o r g a n i se d . Sh o r t t e r m t r a i n i n g p r o g r a m m e s o n va r i o u s
a sp e c t s o f fi sh a n d fi sh e r i e s we r e o r g a n i se d a t Mu m b a i , 4
a t Ko l k a t a , 1 1 a t Ka k i n a d a , 5 a t P o we r k h e d a , 2 a t Ro h t a k
a n d 6 a t Lu c k n o w. At t h e CMFRI , Ko c h i , t r a i n i n g
p r o g r a m m e s we r e o r g a n i se d fo r Se l f He l p Gr o u p o n c r a b
c u l t u r e , m u sse l c u l t u r e , o r n a m e n t a l fi sh c u l t u r e , fi sh
p r o c e ssi n g , m a r i c u l t u r e , fi sh d i se a se a n d t h e i r
m a n a g e m e n t . Th e CI BA a l so c o n d u c t e d t r a i n i n g
p r o g r a m m e s o n b r a c k i sh wa t e r fi n fi sh b r e e d i n g , c r a b
b r e e d i n g a n d c u l t u r e , fi n fi sh b r e e d i n g , so i l a n d wa t e r
q u a l i t y m a n a g e m e n t a n d sh r i m p b r e e d i n g a n d h a t c h e r y
t e c h n o l o g y.
TRAINING PROGRAMMES FOR
RURAL WOMEN
Tr a i n i n g p r o g r a m m e s we r e c o n d u c t e d fo r r u r a l wo m e n
o n fi sh / sh r i m p p i c k l e p r e p a r a t i o n i n Ti r u va l l u r Di st r i c t o f
Ta m i l Na d u u n d e r t h e NATP - I VLP p r o j e c t , Br a c k i sh wa t e r
a q u a c u l t u r e a t Ne l l o r e Di st r i c t , An d h r a P r a d e sh , a n d
p r e p a r a t i o n o f va l u e a d d e d fi sh p r o d u c t s a t Ka t t u r vi l l a g e ,
Th i r u va l l u r.
K