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Media For Cell Culture
Media For Cell Culture
Asad Naz
Minimum requirements
for growing cell
Media
Support survival and growth
Natural Media
Artificial Media
Minimum requirements
for growing cells
Choice of Media
Type of cell culture (Normal, Immortalized)
Object of culture (Growth, survival,
differentiation)
Natural Media
Clots
Plasma clots (male fowl)
Biological Fluids
Amniotic, Ascitic fluid, serum etc
Tissue extracts
Chicken and bovine embryo extracts
Artificial Media
Immediate Survival
Prolonged Survival
Indefinite growth
Specialized function
Classes
Serum containing media
Serum free media
Chemically defined Media
Protein free media
Serum Containing
Media
EMEM with 5-20% serum
Provide plasma protein, peptides, lipids
carbohydrates, minerals and enzyme
Hormones (cortisone, insulin and testosterone and
prostaglandin)
Growth factors (PDGF, TGF-p, epidermal growth factors etc)
Supply protein (fibronectin, spreading factor
Serum Containing
Media
Binding factors (albumin, transferrin)
Increase viscosity of medium
Protease inhibitor
Buffer
Minerals (Na, K, Fe, Zn, and Cu etc)
Disadvantages of
serum
Inhibit growth of some cell type
Cytotoxic constituents (polyamine
oxidase)
Variation in serum quality
May add growth factors
Interfere downstream processing
Lower supply
Serum Free Media
Analytical approach
Synthetic approach
Limiting factor approach
Short term
Thymidine assay
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