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S-MEDB413LA MEB34 1st Sem ( 2022-2023 )

Molecular Genetics of Prokaryotes Problem Set

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Question 1
Different alleles for a bacterial genes.How many lac Z genes were present in the cells described in problem 5 that were able to grow on minimal media?
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Response: Two, the lac Z- allele on the bacterial chromosome and the lac Z+ allele on the F' episome.

Question 2
Exchange of genetic information between bacteria.Cells of an E. coli strain that are trp lac Z met bio were mixed with cells of an E. coli strain that are
trp lac Z met bio and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar
source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become trp lac Z met bio?

Response: conjugation

Question 3
The effect of high glucose on lac operon expression.Bacteria utilize glucose first, even if other sugars are present, through a mechanism called:

Response: catabolite repression

Question 4
The Oc mutation of the lac operon.An E. coli strain that is lac Z is conjugated with E. coli cells carrying an F' plasmid with the plac Oc lac Z DNA
sequence on the episome. The Oc is a mutation of the lactose operator that is no longer able to bind the lac I gene product which codes for the lac
repressor. How would the expression of the lac Z be regulated in the resulting cells that are diploid for the lactose regulatory region and the lac Z gene?

Response: constitutive expression of lac Z+.

Question 5
Effect of high lactose and low glucose on lac operon.Which of the following growth media would you expect to result in synthesis of high levels of
mRNA for the enzymes of the E. coli lac operon?

Response: no glucose, high lactose

Question 6
DNA transfer by an F factor.An E. coli strain is F lac Z met bio. Cells from this strain are mixed with an E. coli strain that is lac Z met bio and carrying
an F' episome with the plac O lac Z DNA sequence on the episome, and cultured for several hours. Then cells were removed, washed, and transferred to
minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did
these few cells become lac Z met bio?

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Response: sexduction, a special type of conjugation

Question 7
Phenotype of cells diploid for lac I gene.An E. coli strain that is lac I is conjugated with E. coli cells carrying an F' plasmid with the PI lac I DNA
sequence on the episome. How would the expression of the lac Z be regulated in the resulting cells that are diploid and heterozygous for the lac I gene
(lac I on episome and lac I on the bacterial chromosome)?

Response: normal regulation of lactose metabolism

Question 8
Effect of the absence of glucose.An E. coli cell is grown in the absence of glucose. Which of the following will be true?

Response: If lactose is present, lac mRNA synthesis will occur at a high level.

Question 9
Phenotype of lac Z- E. coli cellsAn E. coli strain is lac Z. The structural gene for ß-galactosidase is encoded at the lac Z locus. How would you describe
the regulation of lactose metabolism in these cells?

Response: inability to synthesize the lac Z+ gene product, ß-galactosidase.

Question 10
The role of the inducer of the lac operon.The inducer:

Response: combines with a repressor and prevents it from binding to the operator.

Question 11
Components of the lac operon of E. coli.Which of the following is not part of the lac operon of E. coli?

Response: gene for RNA polymerase

Question 12
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Regulation of lac Z gene on an F' episome.How would you describe the regulation of lactose metabolism in the cells described in problem 5 that were
able to grow on minimal media with lactose as a nutrient?

Response: normal regulation of lactose metabolism.

Question 13
Effect of high glucose concentrations.An E. coli cell is grown in the presence of high amounts of glucose. Which of the following is true?

Response: The level of cyclic AMP in the cell will be low.

Question 14
Phenotype of lac I- cells.An E. coli strain is lac I. The lac I DNA sequence encodes the lactose repressor. How would you describe the regulation of
lactose metabolism in these cells?

Response: constitutive expression of lac Z+

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