Gel Loading: 1. % of gel and thickness used: 2. Number of wells: 3.

Sample volume, arker volume:

!. "oltage run, #ime run: $. %un length: &. Sample order: 1 2 3 ! $ & ' ( ) 1* 11 12 13 1! 1$

Transfer: 1. embrane used:

2. #ransfer number +: 3. ,mperes run, #ime run: Ponceau: Stain for 2- with rocking, destain with distilled water. %esult:

Blocking: +

Blotting: 1. .ash membrane ! times with 1/#0S#, $- at %#. 2. 1rimar2 antibod23 )*-, %#, rotate Company: Isotype: Concentration: # Usage: 3. .ash membrane ! times with 1/#0S#, 4nce with 1/#0S, $- at %#. !. Secondar2 antibod2 3 !$-, %#, rotate Company: Isotype: Concentration: # Usage: $. .ash membrane ! times with 1/#0S#, 4nce with 1/#0S, $- at %#. &. Scan IR: