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  • Stripping Blot

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    srividya84
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    The document provides instructions for stripping and reprobing a membrane used for western blotting. It involves washing the membrane, incubating it in a stripping buffer containing beta-mercaptoethanol to remove bound antibodies, blocking the membrane, and then probing it again with primary and secondary antibodies for detection of a different target protein.

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    The document provides instructions for stripping and reprobing a membrane used for western blotting. It involves washing the membrane, incubating it in a stripping buffer containing beta-mercaptoethanol to remove bound antibodies, blocking the membrane, and then probing it again with primary and secondary antibodies for detection of a different target protein.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    7 views1 page

    Stripping Blot

    Uploaded by

    srividya84
    The document provides instructions for stripping and reprobing a membrane used for western blotting. It involves washing the membrane, incubating it in a stripping buffer containing beta-mercaptoethanol to remove bound antibodies, blocking the membrane, and then probing it again with primary and secondary antibodies for detection of a different target protein.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    STRIPPING Membrane used: 1. Wash membrane 4 times with 1X TBS, 5 at RT. 2. Pour 10ml strippin bu!!er into "ish.

    #"" $ul B%mer&aptoethanol. Prein&ubate at 42' !or 5%10. (. Pla&e membrane in strippin bu!!er, 42', )0. 4. Wash membrane 4 times with 1X TBS, 5 at RT. *o this in a lar e "ish !or washin o!! tra&es o! strippin bu!!er. Blocking: +,-, 4' in &ol" room. . Blotting: 1. Wash membrane 4 times with 1XTBST, 5 at RT. 2. Primar/ antibo"/% 00, RT, rotate Company: Isotype: Concentration: # sage: (. Wash membrane 4 times with 1XTBST, +n&e with 1XTBS, 5 at RT. 4. Se&on"ar/ antibo"/ % 45, RT, rotate Company: Isotype: Concentration: # sage: 5. Wash membrane 4 times with 1XTBST, +n&e with 1XTBS, 5 at RT. ). Scan IR:

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