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  • 12/TEACHERSGUIDE/PLANTBIOTECHNOLOGY/Pages/Activity4.aspx-: - WWW - Nps.gov/public - Health/info/... /colilert PDF

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    The document discusses Enterobacteriaceae, the family name of coliform and E. coli bacteria. It describes how the pGREEN plasmid causes bacteria to fluoresce under UV light, containing genes for both GFP and antibiotic resistance. It provides instructions that if a water sample has background color, compare it to an uninoculated blank of the same water, and use sterile water rather than buffered water to dilute samples before adding Colilert, as it is already buffered.

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    lab

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    The document discusses Enterobacteriaceae, the family name of coliform and E. coli bacteria. It describes how the pGREEN plasmid causes bacteria to fluoresce under UV light, containing genes for both GFP and antibiotic resistance. It provides instructions that if a water sample has background color, compare it to an uninoculated blank of the same water, and use sterile water rather than buffered water to dilute samples before adding Colilert, as it is already buffered.

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    7 views1 page

    12/TEACHERSGUIDE/PLANTBIOTECHNOLOGY/Pages/Activity4.aspx-: - WWW - Nps.gov/public - Health/info/... /colilert PDF

    Uploaded by

    messiorlando
    The document discusses Enterobacteriaceae, the family name of coliform and E. coli bacteria. It describes how the pGREEN plasmid causes bacteria to fluoresce under UV light, containing genes for both GFP and antibiotic resistance. It provides instructions that if a water sample has background color, compare it to an uninoculated blank of the same water, and use sterile water rather than buffered water to dilute samples before adding Colilert, as it is already buffered.

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    © All Rights Reserved
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    QUESTIONS: 1. What is the family name of coliform and E.coli group?

    Enterobacteriaceae

    2. What is the use of UV light ? Why that bacteria must be observed under UV light? The pGREEN plasmid contains a mutant version of GFP linked to another gene called beta-galactosidase. This combination of genes was chosen because the protein produced from this combination turns bacteria yellow-green, even in normal light. If we expose the colonies to a UV light, they also fluoresce. The plasmid also contains the antibiotic resistance gene to allow growth in the presence of ampicillin. http://www.apsnet.org/EDCENTER/K12/TEACHERSGUIDE/PLANTBIOTECHNOLOGY/Pages/Activity4.aspx-

    3. What to do if water sample has some background color? How to make the dilution? If a water sample has some background color compare inoculated colilert sample to control blank of the same water sample. Use sterile water, not buffered water for making dilutions. Colilert is already buffered. Always add Colilert to the proper volume of diluted sample after making dilutions.

    http://quizlet.com/11787504/water-monitoring-flash-cards/ www.nps.gov/public_health/info/.../colilert.pdf

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