Metabolism in Skeletal Muscle

Glycolysis
Glycogenolysis -oxidation (ketone bodies)

Krebs (tricarboxylic acid) cycle

Branched-chain amino acids Electron transport chain

Calcium regulation
Key enzyme regulation
Eric Niederhoffer SIU-SOM

Pathways Overview
Glucose Glycogen
Glycogenolysis Ca2+ PKa Glycolysis

Ketone bodies Fatty acids -Oxidation

Ca2+ PDH

G6P

Lactate

No O2

Pyruvate

Acetyl-CoA

BCAA Ile, Leu, Val

Krebs cycle
Ca2+ ISDH, aKGDH

Electron Transport Chain

Production of ATP

Regulation in Skeletal Muscle

Ep AR AC cAMP PKA ATP

Glc G6P F6P

Glycolysis

ATP Citrate PFK-2 F26BP

NH4+ AMP Pi

Ca2+ PKa PP
Pi IMP AMP

PFK-1 F16BP

Glycogen
Glycogenolysis

PEP
PDHP Ca2+ PDHP PDH PDHK

PK Pyr PDH

-Oxidation Fatty acids Acetyl-CoA

Review Questions

How does muscle produce ATP (carbohydrates, fatty acids, ketone bodies, branched-chain amino acids)? How is skeletal muscle phosphofructokinase-1 regulated? What are the key Ca2+ regulated steps?