CHECKLIST FOR DSMZ SERVICES

Analysis of the cellular fatty acid composition Plate / fresh culture

Analysis of respiratory quinones dried cells, in our case fresh cultures

Analysis of polar lipids Freeze dried cells, in our case fresh cultures

Analysis of peptidoglycan structure For peptidoglycan analysis 3-4 g wet weight of cells (centrifuge pellet) is needed. The wet biomass can be sent either in frozen state on dry ice or suspended in isopropanol/water (1:1, v/v) at ambient temperature. DNA - DNA hybridization 3g of wet biomass from all strains included in the analysis are needed. It must be suspended in iso-propanol / water (1:1, v/v) (first suspend biomass in water, mix well and then mix 1:1 with isopropanol) which can be sent in tightly sealed screw cap tubes (e.g. 50 ml Falcon tubes) at ambient temperature. Alternatively centrifuged biomass might be sent on dry ice (best option).