Optimization of Sanitizing

Process Using Heat Against

Bacteria Asaia bogorensis
Petra Klodnerov, MSc
Supervisor: Miroslav eovsk, MSc, PhD
INSTITUTE OF CHEMICAL TECHNOLOGY, PRAGUE
Department of Food Preservation and Meat
Technology
Asaia bogorensis
Fig. 1: Colonies on PCA Agar.
Fig. 2: Microscopic picture of cells.
Aim of Research
Heat Resistance of A. bogorensis
technological problem
Asaia bogorensis = new unusual spoilage
organism of still fruit-flavored bottled
water

sanitizing process optimalization, using
heat
find D and z value

Thermal Inactivation Parameters
D and z value

0.5
1
1.5
2
2.5
3
3.5
Log D
z
Temperature
0 2 4 6 8 10 12 14

Time
1
2
3
4
5
6
7
D
Log N
Fig.3: Survivor curve Fig.4: Thermal-death-time curve
Methods and Instruments
41 mm
10 mm
12 mm
Silicon gasket
135 mm
1.5 mm
1.9 mm
Metal capillary tube
Holder of
capillary tube
Teflon
piston
Fig. 5: TDT tube method
Fig. 6: Equipment for
capillary tube method
Experimental Procedure

tube submersion in heating water bath


tube immersion in ice-water bath


heated sample microbial analysis

construction of microbial survivor
curve and thermal-death-time curve

TDT and capillary tube filling with sample

D and z value
sample preparation suspension
cultivated 96 hr, 30 C
Temperat
ure [C]
D value [min]
TDT tube
capillary
tube
45 20.5 3.9 24.5 7.9
50 4.6 1.8 4.5 1.3
55 0.6 0.2 0.6 0.1
z value
TDT tube
method
6.5 C
Capillary tube
method
6.2 C
D and z Values of Asaia
bogorensis in Nutrient Broth
Comparison of TDT Tube Method
and Capillary Tube Method
-1
0
1
2
40 42 44 46 48 50 52 54 56
Temperature [C]
Log D
TDT tube capillary tube
D
50
Value of Asaia bogorensis
in McIlvaine Buffer
pH D
50
value
3.4
6.74 1.42
5
8.51 1.54
8
5.57 0.79
CONCLUSIONS
A. bogorensis is more heat resistant after
cultivation at 30 C than at 25 C.
A. bogorensis is sensitive to relatively low
temperatures (D
55
= 0,6 min).
Heat resistance of A. bogorensis at 50 C is
the least at pH 8.
Possibility of heat treatment usage for
optimalization of sanitizing process, whereas
thermally sensitive components of
equipment are not damaged.

Thank You for Your
Attention