Professional Documents
Culture Documents
Toxins can disturb biochemical pathways and may have a role in disease
development. Removing toxins, harmful or potentially damaging substances from our bodies and
our diet helps us to stay fit and healthy. This process is called detoxification, which can also
country and now spreading across Asia due to information dissemination via web internet. It is
easily made at home and its ingredients vary according to the preferred taste of the consumer.
This drink usually consists of water as its base liquor, and organic foods (fruits, vegetables,
herbs). Lemon is the commonly used ingredient for this drink, however, calamansi will be its
In this proposal, the detox drink that will be prepared will contain water, cucumber,
calamansi, and honey. Due to its wide variety of health benefits (antioxidant property, good
source of Vitamin C and E), everybody can be a beneficiary from this drink.
Since this drink is composed of biological raw materials, thus, it inherently spoils and
deteriorates over time. This spoilage and deterioration cannot be completely stopped; however, it
is the desire of food processors to slow this rate of deterioration as much as possible through
Specific objectives:
1. To prepare a packed detox drink using cucumber, calamansi, water, and honey;
3. To perform shelf life analysis based on its total soluble solids, color, Vitamin C
Toxin. It obstructs or delays the normal functions of our bodies, or causes stagnation,
congestion or disease. Toxins can come from food or water, from chemicals used to grow or
prepare food, and even from the air that we breathe. Our bodies process those toxins through
organs like the liver and kidneys and eliminate them in the form of sweat, urine, and feces. Aside
from eliminating them, other mechanisms for getting rid of toxins are by neutralizing or
transforming it into harmful one. These processes falls under a process called Detoxification.
Some of those toxins that enter our body are: methane, carbon monoxide, chlorine, bleach,
ammonia, food additives, monosodium glutamate, aspartame, pesticide residue, mercury, lead,
and a lot more. These toxins, when taken up (unknowingly or not) regularly, will build up in our
filtering organs like kidneys, liver, and lungs, thus, making our organs weaker for its function.
Detox Drink. A detox cleans out body waste deposits. It helps out the liver by stimulating
its detoxification process to be more efficient. After cleansing, the body starts rebalancing and
fleshy, prostrate or climbing vine. Phytochemical screening yielded flavonoid which has
antioxidant property and anti-cancer activity. Sliced cucumber usually lasts for 1-2 days. It is
Vitamin C which has an antioxidant property that stimulates the release of the enzymes that
are essential part of the liver’s detoxification process, encouraging it to flush our unwanted
Honey. It has been proven that bacteria cannot live in the presence of honey because it is
an excellent source of potassium which withdraws from the bacteria the formation of moisture
which is essential to their very existence. It has the property of acting as a catalyst to the
Shelf life. A food is considered spoiled when it is no longer acceptable to the consumer.
Less serious cases of food spoilage can simply be that the color, flavor, texture, or aroma of
the food has deteriorated to the point that it is no longer acceptable. Another case is when the
nutrients in the food have deteriorated to the point that the food no longer meets its declared
nutritional value.
Packaging. Packaging materials used for food liquids should maintain good hygiene and
have sufficient mechanical strength to prevent leakage and contamination from the outside.
They should also be inert and provide a barrier to light. (Paine, 2012)
CONCEPTUAL FRAMEWORK
Sample Preparation
Screening test
Packaging
METHODOLOGY
Sample Preparation
Each sample will be prepared with 1 Liter of distilled water added with 100 grams of
Experimental Treatments
Each prepared samples will be added with different weight in grams of honey (0, 50, 100,
150, 200), packed in glass bottles covered with foil, and will undergo the screening tests.
Screening Tests
The screening tests are pH determination, Vitamin C content determination, total soluble
Sensory Evaluation
years of age. Prior to sensory evaluation, the treated samples will be allowed to fuse for 1
hour in a refrigerator, randomly coded, and served (20 mL) together with 100 mL distilled
water. Treated samples will be compared in terms of color, sweetness, flavor, and overall
acceptability.
Packaging
The sample will be packed in an aseptically cleaned and tightly sealed Polyethylene
bottle.
The most favored treatment from the screening process will go through the shelf life
analysis, wherein, 2 Liters of the treatment will be prepared and exposed in different
temperature conditions in degrees Celsius (5, 10, 25, 30) with 3 replicates each treatments for
2 weeks. Every 24 hours, starting from prior to exposing the treated samples under different
temperature conditions, shelf life will be analyzed based on its color, Vitamin C content
determination, pH determination, microbial test, and total soluble solids. As for microbial
soluble solids, Vitamin C content, and Color, methods will be used are hand refractometer,
In measuring total soluble solids, the refractometer prism surface should be ensured as
clean and dry. A small amount of the treated sample will be placed on the prism surface. Look
through the eyepiece while pointing the prism in the direction of a good light (not directly at
the sun). Focus and take the reading of where the base of the blue colour sits on the scale and
record the % percentage sugar (°Brix). Clean the refractometer immediately with a damp
tissue, dry thoroughly, and repeat the step for the other samples.
For redox tritration, Iodine solution and starch indicator solution will be prepared.
Iodine solution: (0.005 M). Weigh 2 grams of potassium iodide into a 100 mL beaker.
Weigh 1.3 grams of iodine and add it into the same beaker. Add a few mL of distilled water
and swirl for a few minutes until iodine is dissolved. Transfer iodine solution to a 1 L
volumetric flask, making sure to rinse all traces of solution into the volumetric flask using
distilled water. Make the solution up to the 1 L mark with distilled water. (*The concentration
of the prepared iodine solution can be more accurately determined by titration with a standard
Starch indicator solution: (0.5%). Weigh 0.25 grams of soluble starch and add it to 50 mL
of near boiling water in a 100 mL Erlenmeyer flask. Stir to dissolve and cool before using.
Pipette a 20 mL the sample solution into a 250 mL Erlenmeyer flask and add about 150
mL of distilled water and 1 mL of starch indicator solution. Titrate the sample with 0.005 M
iodine solution. The endpoint of the titration is identified as the first permanent trace of a dark
For spectrophotometer, turn it on and allow to warm up for 5 to 10 minutes. Select the
wavelength. With the sample compartment closed and empty, adjust the % Transmittance
(zero percent transmission of light) to read 0% T using the left front dial. Place a clean (no
fingerprints), dry cuvette filled approximately 3/4 full of the blank sample (solvent only) in
the sample compartment. Close the sample compartment. Adjust the % Transmittance to read
100% T (100 percent transmission of light) using the right front dial. Remove the blank
cuvette and place the cuvette containing the sample in the sample compartment. Close the
sample compartment. Read and record the value registered on the meter. (*Every time the
wavelength of light is changed, the instrument must be recalibrated to read 0% T and 100% T
As for the Microbial Test, the media to be used is the Potato Dextrose Agar (PDA). In
Preparing the PDA, 39 grams of dehydrated PDA will be suspended in 1 Liter distilled water.
Heat mixture to boiling, then distribute to flasks, and autoclave for 15 minutes at 121 degrees
Celsius. Streptomycin and Chloramphenicol at a final concentration of 100 ppm are added just
before pouring.
In isolation of yeasts and molds, 10 mL of juice sample will be diluted with 90 mL of
0.1% sterile peptone water (1 g peptone, 1L distilled water). *First Dilution
Transfer 1 milliliter from the first dilution to 9 milliliter of 0.1% peptone water. *Second
Dilution
Prepare another dilution by transferring 1 milliliter from the second dilution to 9 milliliter
Inoculate 0.1 milliliter of the three dilutions in duplicate onto solidified PDA with
antibiotics. Spread the inoculums over the entire surface of the agar using a sterile bent glass
rod. Incubate plates in an upright position for 5 days at 25-30 degrees Celsius. Count plates
containing 15-150 colonies. If mold overgrowth has occurred, count from the underside of the
plate.