Reducing sugar analysis

Reagent Preparation:
2.5gm of sodium hydroxide dissolve in 120ml of water (every thing should be
covered in aluminum foil). 3.2gm of initro salicyclic acid (!") mix it and #ee$ it
for 10mins% then &gm of "odium 'otassium tartarte mix it store in brown bottle
and ma#e u$ to 200ml.
Standard Preparation(
1gm of extrose anhydrous in 100ml water (1))
*a#e 2ml from 1) solution and ma#e u$ to 100ml (0.02) 50 dilution so 1+50 it
gives 0.002)
,ml 1) solution - .&ml water 0.00,)
&ml 1) solution - .,ml water 0.00&)
/ml 1) solution - .2ml water 0.00/)
Glucose analysis Protocol:
0.5ml sam$le - 0.5ml !" #ee$ it for 10mins boiling water bath 100 degree
0lan# 0.5ml istilled water - 0.5ml !"
!" react with glucose and it will give color% after 10mins cool it.
1dd 5ml of istilled water and chec# the 2 at 5,0nm
Sucrose analysis Protocol:
*a#e 2ml of sucrose sam$le% add ,0ul of conc 3cl% and #ee$ in water bath for
5mins 4 100 degree c
1fter 5mins% add 100ul of 55 623
1fter neutrali7ing% continue the !" method.