225 mL Listeria enrichment broth Blend at 10,000-12,000 rpm for 2 min Listeria colonies are black with halo on esculin-containing media
After 4 h at 30 o C in buffered Listeria Enrichment Broth (LEB), add selective agents acriflavin, 10mg/L, sodium naliixate, 40mg/L, cycloheximide 50mg/L or natamycin, 25mg/L FOOD ANALYTICAL SERVICE LABORATORY FOOD AND NUTRITION RESEARCH INSTITUTE MIC-AM-007- Incubate at 30 o C for 24-48 hr; streak plates at 24 hr and after 48 hr
Streak BLEB onto OXA. Incubate at 35 o C for 24-48 h
Use rapid kits for biochemical testing to genes or species level.
Transfer 5 or more typical colonies from OXA to trypticase soy agar with yeast extract (TSAYE) streaking for purity, incubate at 30 o C, 24-48 h. Plates may be incubated at 35 o C if colonies are not used for wet mount motility. oxa oxa or PALCAM agar PALCAM agar