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  • Serum Plate Agglutination SPA Test Demo E4b

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    Ryan Devantara
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    The document describes the Serum Plate Agglutination (SPA) test, a rapid agglutination test used to detect antibodies to various Avian Mycoplasmas in chickens and turkeys. The SPA test uses wells containing Mycoplasma particles that will agglutinate, or clump together, if the serum sample contains antibodies specific to M. gallisepticum, M. synoviae, or M. meleagridis. A positive result is indicated by agglutination in the bottom wells, while the top wells will remain clear without agglutination if the sample is seronegative, or negative for specific antibodies.

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    SPA Test

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    The document describes the Serum Plate Agglutination (SPA) test, a rapid agglutination test used to detect antibodies to various Avian Mycoplasmas in chickens and turkeys. The SPA test uses wells containing Mycoplasma particles that will agglutinate, or clump together, if the serum sample contains antibodies specific to M. gallisepticum, M. synoviae, or M. meleagridis. A positive result is indicated by agglutination in the bottom wells, while the top wells will remain clear without agglutination if the sample is seronegative, or negative for specific antibodies.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    25 views1 page

    Serum Plate Agglutination SPA Test Demo E4b

    Uploaded by

    Ryan Devantara
    The document describes the Serum Plate Agglutination (SPA) test, a rapid agglutination test used to detect antibodies to various Avian Mycoplasmas in chickens and turkeys. The SPA test uses wells containing Mycoplasma particles that will agglutinate, or clump together, if the serum sample contains antibodies specific to M. gallisepticum, M. synoviae, or M. meleagridis. A positive result is indicated by agglutination in the bottom wells, while the top wells will remain clear without agglutination if the sample is seronegative, or negative for specific antibodies.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    4b.

    Serum Plate Agglutination (SPA Test)


    SPA test is a serological
    rapid agglutination test
    for Avian Mycoplasmas
    MG/MS in chickens M.
    gallisepticum/synoviae
    MG/MS/MM as above and M.
    meleagridis in turkeys

    Top wells are seronegative (no
    specific antibodies)

    Botton wells are sero-positive
    (specific antibodies have cross-
    linked Mycoplasma particles
    and caused agglutination)
    VPM 201: Mycoplasma Laboratory 10-2011

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