Circadian

rhythm

March 14
1088

tongli zhang

Circadian rhythm

Circadian rhythms in different

organisms

 Control network in mammalian

Circadian rhythms in
Cyanobacyteria

cyanobacteria

Their interaction
KaiA, KaiB and KaiC interact with each
other, forming large complexes in vivo.
KaiC is the core of the complex.
The complex is mainly at night.

kaiC

KaiA
kaiA enhances KaiC phosphorylation
KaiA has two domains- N-terminal is a
pseudo-receive domain
N-terminal domain of KaiA does not
promote KaiC autophosphorylation
KaiA from anabaena even does not have a
N-terminal domain
However, mutation of N-terminal domain
of KaiA lengthen the circadian period

KaiA 2
KaiA C-terminal binds KaiC and promotes
its phosphorylation
One KaiA is enough to enhance the
phosphotylation of one KaiC hexamer

KaiA 3

KaiA exists as a domain-swapped homodimer

KaiA's pseudo-receiver domain
The C-terminal domain of KaiA, C-KaiA, is all ahelical and adopts a novel dimeric structure.

KaiA 4 open and close

kaiA dimer
N of one and C of
another interact
This is closed
When the interaction
is broken- KaiA dimer
is open
Closed is supposed to
be the active form

kaiC binding with KaiA

kaiB
Positively charged residues R23 and K75

kaiC binds with KaiB

The potentials (positive, blue; negative,
red)

A rotary clock

Happy everyone?

What about
1. entrainment?
2. temperature compensation?
3. fitness?
4. cancer development?

Way to solutions.