A Dose Response Study with Lactylate in Broilers

Challenged with a Subclinical Clostridium Infection

M. Lensing*, J.D. van der Klis* and A. Cazemier
* Schothorst Feed Research, Lelystad, The Netherlands, PURAC, Gorinchem, The Netherlands

Introduction and Objectives

Results

A lactylate is an ester of a fatty acid and the

hydroxyl group of lactic acid and is of interest as
a feed additive because of its distinct
antimicrobial action. A dose response study was
performed to determine the efficacy of lactylate
(PURAMIX
30
produced
by
PURAC,
the
Netherlands) in a subclinical Clostridium infection
model.

Materials and Methods

NINF 0.3

Infected

INF

Infected + 0.60% PURAMIX 30

INF 0.6

Infected + 0.30% PURAMIX 30

INF 0.3

Infected + 0.15% PURAMIX 30

INF 0.15

Infected + 0.075% PURAMIX 30

INF 0.075

Infected + 0.038% PURAMIX 30

INF 0.038

Infected + 0.019% PURAMIX 30

INF 0.019

10

Infected + 0.010% PURAMIX 30

INF 0.010

11

Infected + 0.005% PURAMIX 30

INF 0.005

Figure 2. Effect of Puramix 30 on mean severity of lesions scored in all

necropsied birds at days 15, 16 and 17 (i.e. 1, 2 and 3 days p.i.). Points
without common letter differ significantly (P < 0.05). Blue point is INF
treatment.

Measurements:
NE incidence, lesion score, body weight gain (BWG), daily feed
intake (FI), feed conversion ratio (FCR)

Mortality
25

ab

ab

ab
ab

15

ab

abc

10

cd

d
NINF 0.3

NINF

PURAMIX 30 (lactylate) is effective to control

Clostridium infections in broilers. It reduces the
incidence of necrotic enteritis and the severity of
lesions. Although PURAMIX 30 did not affect
production performance, mortality rate was
significantly lower.

Mortality (%)

Conclusion

20

INF 0.005

Not infected + 0.3% PURAMIX 30

INF 0.010

NINF

INF 0.019

Code

Not infected

INF 0.038

Description

INF 0.075

Trt

INF 0.15

Treatments:

INF 0.3

Infection:

INF 0.6

Feed:

1,188 male Ross 308 day-old broilers

11 treatments x 6 replicate litter cages
(18 birds per cage)
wheat/barley-based diet
(AME: 11.9 MJ/kg and dLYS: 9.6 g/kg)
104 sporulated E. maxima oocysts (day 9),
108 cfu C. perfringens (day 14)

INF

Animals:
Setup:

Figure 1. Effect of Clostridium infection (indicated by blue arrow) on daily

feed intake NINF vs INF (left) and example of affected duodenal/jejunal
intestinal tissue (right).

Figure 3. Mortality (%) per treatments from day 9 to 37. Columns without
common letter differ significantly (P < 0.05).

Schothorst Feed Research