Power Point

Intro. To micro-array (include structure of the gene.

Micro-array-Process/Benefits
mRNA sequence Process/Benefits
mRNA vs micro-array

What I plan to do:

Check the expression of cell cycle genes.
How (Talk about Cancer Cell Lines-Article-what is it? And where is it available? Use some real-life
examples.)
Using the CCL data to find the specific to the cell cycle; find which are necessary.

These are the Genes are going to be checked using the CCL:
ORC1, ORC2, ORC6, CDC45, MCM6, MCM7, CDC25A, MCM9, RPA2, DBF4
(Do table left column: specific gene top rows: cell lines)
Purpose: To see if they are not expressed (deleted/ mutated/). If so, then that specific gene would be
classified as an essential cell (would be a significant finding)
DONT FORGET TO CITE THE SOURCES!!!!!!
http://en.wikipedia.org/wiki/Oligonucleotide
http://www.nature.com/nature/journal/v483/n7391/full/nature11003.html
http://nitro.biosci.arizona.edu/courses/EEB600A-2003/lectures/lecture24/figs/euk.jpg
http://www.broadinstitute.org/ccle/data/browseData?conversationPropagation=begin

[1]

Weiss, B., ed. (1997). Antisense Oligodeoxynucleotides and Antisense RNA : Novel

Pharmacological and Therapeutic Agents. Boca Raton, FL: CRC Press.

[2]

Watanabe, T., Miura, T., Degawa, Y., Fujita, Y., Inoue, M., Kawaguchi, M., & Furihata, C. (2010).

Comparison of lung cancer cell lines representing four histopathological subtypes with gene
expression profiling using quantitative real-time PCR. Cancer Cell International. doi:10.1186/14752867-10-2

[3]

Browse Data. (n.d.). Retrieved November 20, 2014, from

http://www.broadinstitute.org/ccle/data/browseData?conversationPropagation=begin

[4]

Malone, J., & Oliver, B. (2011). Microarrays, deep sequencing and the true measure of the

transcriptome. BMC Biology, 34-34.