BVU-RGITBT

B.Sc. Biotechnology Sem V Test No.2

Subject Genetics. Topic Chromosome

1. The given karyogram is of

a. Down syndrome male, b. Down syndrome female, c. Turners Syndrome male, d. Turners
syndrome female
2. Which of the following techniques is used in Chromosome painting?
a. Chromosome isolation, b. FISH, c. ELISA, d. All are correct
3. Short arm of a chromosome is called as
a. p arm, b. q arm , c. centromere,
4. In acrocentric chromosome, centromere is at ?
a. middle, b. off middle, c, at the end,
5. In a band of metaphase chromosome, dark bands are regions of ?
a. highly condensed regions, b. loose regions, c. Region of maximum activity, d. Region of less
activity.
6. Explain the principle of high resolution banding.
7. Explain various applications of FISH.
8. Explain the applications of Karyotyping
9. Which one in the following is the correct sequence of steps for karytyping
a. Sampling, culturing of monocytes with Phytohemagglutinin, treatment with Colcemid, hypotonic
treatment, fixation of swollen chromosome, staining, microscopy and arrangement of chromosome.
b. Sampling, culturing of monocytes with Colcemid , treatment with Phytohemagglutinin, fixation of
swollen chromosome, hypotonic treatment, staining, microscopy and arrangement of chromosome
c. Sampling, treatment with Colcemid, culturing of monocytes with Phytohemagglutinin, hypotonic
treatment, fixation of swollen chromosome, staining, microscopy and arrangement of chromosome
d. Sampling, culturing of RBCs with Phytohemagglutinin, treatment with Colcemid, fixation of
swollen chromosome, hypotonic treatment, staining, microscopy and arrangement of chromosome.