Connie Kutch (10893551)

micro
1. A. EMB- you know something will grow if its from B. MacConkey Agar or Xylose
Lysine Desoxycholate. If the culture is not shigella you will get growth on the media
listed in 2, whereas you should get growth in 1. If it is E. coli, in particular the
growth in 1 should be metallic and green.
2. Take the mixed culture and grow it in a broth so the numbers are high and happy.
You are going to use selective media to separate them with the T streak method and
isolate the pseudomonas. You will use the pseudomonas isolation agar. For the
staph, grow on mannitolsalts. S. aures will change the media yellow the others will
not. To then isolate the individual strains select individual CFU, 1 red and 1 yellow.
Once you have isolated the 3 individual bacteria, you can inoculate 3 separate
media broths, one each with one of the bacteria.
3. By day 6 you will have a full bloom and you can do plate counts after doing serial
solutions instead of doing microscope direct counts.
4. By noon 64 bacteria will be present and if you wait until 20 generations have
passed you will begin any time after 7. They will all die off because of decreased
nutrients and increased waste.
5. Last years dilution count was the 1:100 ratios and it yielded roughly 49 CFUs
which would suggest there were approximately 4900 bacteria per ml of sample last
year. This year you would select 1:1000 dilutions and the count was roughly 45,
which would indicate approximately 45,000 bacteria per ml of sample this year. The
results suggest that there is a marked increase in bacteria and that research must
be done to determine what can be causing the boom in bacteria.