Robert A.

Weinberg

The Biology of Cancer

First Edition

Chapter 4:
Cellular Oncogenes

Copyright Garland Science 2007

Transfection

C3H10T1/2 cells

3-methylcholanthrene
(3-MC)

Figure 4.2 The Biology of Cancer ( Garland Science 2007)

NIH3T3 cells

Transformation of mouse cells by human tumor DNA

Transfection of NIH3T3 cell with DNA of T23 human bladder carcinoma cell line

Figure 4.3 The Biology of Cancer ( Garland Science 2007)

Northern and Southern

Blotting Procedures

Figure 4.4 The Biology of Cancer ( Garland Science 2007)

Homology between transfected oncogenes and retroviral oncogenes

1)
2)
3)

Southern blot
Probe with H-ras
NIH3T3 cells by transfection with human bladder tumor DNA

Figure 4.5 The Biology of Cancer ( Garland Science 2007)

Table 4.1 The Biology of Cancer ( Garland Science 2007)

Amplification of the erbB/neu oncogene in breast cancers

(A) Southern Blot

(B) Kaplan
Kaplan-Meier
Meier Plot

Amplification of the erbB/neu oncogene in breast cancers

Amplification of the erbB/neu oncogene in breast cancers

A li
Amplicon

Cloning of transfected human oncogne

Figure 4.8 The Biology of Cancer ( Garland Science 2007)

Localization of an oncogene-actvating mutation

Figure 4.9 The Biology of Cancer ( Garland Science 2007)

Mutation responsible for H-ras oncogene activation

Figure 4.10 The Biology of Cancer ( Garland Science 2007)

Table 4.2 The Biology of Cancer ( Garland Science 2007)

N-myc amplication and childhood neuroblastomas

Figure 4.11a The Biology of Cancer ( Garland Science 2007)

Table 4.3 The Biology of Cancer ( Garland Science 2007)

Burkitts lymphoma in Africa

Figure 4.12 The Biology of Cancer ( Garland Science 2007)

Chromosomal Translocation in Burkitts lymphomas

Figure 4.13a The Biology of Cancer ( Garland Science 2007)

Chromosomal Translocation in Burkitts lymphomas

Figure 4.13b The Biology of Cancer ( Garland Science 2007)

Table 4.4 The Biology of Cancer ( Garland Science 2007)

Figure 4.15a The Biology of Cancer ( Garland Science 2007)

Figure 4.15b The Biology of Cancer ( Garland Science 2007)

Table 4.5 The Biology of Cancer ( Garland Science 2007)