oie O15 ath ee SO at oe) ‘Standard Operating Procedure - Orogenic eM soy one 83 Basic IC — Organic ack on SB me om LE Tm Preparation of Reagent ne eee Sulfuric Acid Eluent ~ Organic acid column ( 0.5 mM H,SO, Bret wtedat ~ Fla . Use the micropipette to measure 1000ul of. Ssmoye sulfuric acid into a 1 liter volumetric flask which filled with 500mL CO, free UPW. 2. Top up to the mark with UPW and mix well 3, Degas the eluent for 10 mins. ‘Suppressor Regeneration Solution ~ 0.05M LiCl 1. Weigh 2.1197g of Lithium Chloride. 2. Transfer the LiCl into a 1 Litre volumetric flask which filled with 500 ml. CO; free UPW. 3, Top up to the mark with UPW and mix well. Standard solution = 2.0 ppm 1. Pipette 200ut of acetate standard 1000 ppm with micropipette into a 100 ml volumetric flask. 2. Pipette 200uL each of formate, propionate and butyrate standard 1000 ppm into the same volumetric flask. 3. Top up to the mark with UPW. Standard solution — 5,0 ppm 1. Pipette 500uL of acetate standard 1000 ppm with micropipette into a 100 mL volumetric flask. 2. Pipette 500uL each of formate, propionate and butyrate standard 1000 ppm into the same volumetric flask. 3. Top up to the mark with UPW. | Standard solution ~ 10.0 ppm 1. Pipette 1000uL of acetate standard 1000 ppm with micropipette into a 100 mL volumetric flask. 2. Pipette 1000ul each of formate, propionate and butyrate standard 1000 ppm into the same volumetric flask. 3. Top up to the mark with UPW.Standard Operating Procedure Metrohm 883 Basic IC 1. Switch on computer and 883 Basic IC. 2. Double click at the MagiC Net 2.3 ico 3. Aworkplace window is opened. Se =——— owe —— ———————————Click at the method selection button to open method window. Equilbration | > Start HW |} li Stop HW Method [carbonate K Highlight the desired method and click open, eontowe Geiser Let IC conditioning for 1 hour in the morning before sample injection.8. Check if the detector waste and two suppressor wastes are flowing out. 9. Wait for the pressure and conductivity stable (pressure 4 Mpa, conductivity 90 - 100 uS/em2).. Ce Pump Pressure Injector Postion Conductivity detector 1 Conductivity Pump Flow 10, Click at the Single Determination tab to open the page [F cautbrotion [gngedeternnatis) | Ostenneton sees >Start HW |! l'Stop HW |)11 Select the working method. Filln the sample info; sample ID, sample type, position, injections, volume, dilution and sample info. Determination series status [READY ‘Single determination G 12. Click start button to start the analysis. 13. A message box appeared, inject sample and click ‘Continue’ (035-523 Program stopped : ‘The subprogram Nan program of th time program has been stopped Press [Continue] to continue the program. PLEASE INJECT SAMPLE & PRESS CONTINUE 14, Wait for the analysis to finish and repeat step 11-13 for others standards and samples,15. To check the result. Please click at the Database button. IC Magic Net 2. ee Determination series carbonate G Configuration17. After all samples have been finished, to shut down the IC, click at workplace lick at ‘Stop HW” at Equilibration tab. Run Equilibration | single determination > Start HW || stop HW Method [carbonate 19. Close the MagiC Net software. 20. Shut down the IC and computer.