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Guinita
BS Chem-3
10-11-14
Absorbance
-0.0001
0.0043
0.0104
0.0204
0.0266
0.0310
0.0014
Calibration Curve
0.035
y = 0.0264x - 0.0004
R = 0.9987
0.03
Absorbance
0.025
0.02
0.015
0.01
0.005
0
-0.005
0.2
0.4
0.6
0.8
1.2
1.4
Concentration of Pb (ppm)
B. Determination of Pb. The following data were obtained in the determination of lead in the
(1) unknown, (2) unknown + 0.8 ppm Pb, and (3) 0.8 ppm Pb:
1. Unknown
Trial
1
2
3
Average
Absorbance
0.0012
0.0006
0.0014
Concentration
(ppm)
0.0611
0.0384
0.0686
0.0560
Mass of Pb
(g)
3.06x10-6
1.92x10-6
3.43x10-6
2.80x10-6
% Pb
2.53x10-4
1.70x10-4
3.25x10-4
2.49x10-4
Concentration (ppm)
Mass of Pb (g)
0.8751
0.8865
0.7956
0.8524
2.188x10-5
2.216x10-5
1.989x10-5
2.131x10-5
0.0227
0.0230
0.0206
3. 0.8 ppm Pb
Absorbance
0.0204
Concentration
0.8000
2 1
3
100)
Trial
1
2
3
Average
%R
94.1
101.2
82.3
92.5
Mass of
empty
crucible (g)
1
2
31.2417
37.2616
Mass of Pb (g)
2.000x10-5
Mass of
crucible +
sample
before
heating (g)
32.2425
38.2670
Mass of
crucible +
sample after
heating (g)
Mass of
sample (g)
% moisture
32.1255
38.1478
1.0008
1.0054
11.69
11.86
E. Corrected percent lead after taking % moisture into account (using the formula
%
%
100
100%
Original % Pb
2.49x10-4
Corrected % Pb
2.20x10-4
Conclusion
The lower detection limit of the instrument is 0.004 absorbance units, and the readings
from the unknown are less than the LDL, which makes the entire determination invalid. Since the
readings are below detection limit, it can be concluded that the amount of lead absorbed by the
plant sample from the soil was planted was minimal.