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  • Vetlab Info (1) : Samples For Laboratory Testing Against Avian Pathogens

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    This document provides guidance on sample collection and testing for avian pathogens at VetLab. It outlines which samples and sample types are needed for different types of laboratory tests, including serology, bacteriology, virus isolation, histopathology and molecular biology. For serology, a minimum of 0.5 ml of serum is needed, while various organs such as the heart, liver, spleen and joints can be sampled for bacteriology tests like Salmonella. Different virus diseases require samples from specific organs to be tested via virus isolation, histopathology, or PCR methods. Samples should not be frozen except where specified, and transport conditions like freezing or formalin depend on the intended test.

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    VETLAB

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    Vetlab Info English

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    This document provides guidance on sample collection and testing for avian pathogens at VetLab. It outlines which samples and sample types are needed for different types of laboratory tests, including serology, bacteriology, virus isolation, histopathology and molecular biology. For serology, a minimum of 0.5 ml of serum is needed, while various organs such as the heart, liver, spleen and joints can be sampled for bacteriology tests like Salmonella. Different virus diseases require samples from specific organs to be tested via virus isolation, histopathology, or PCR methods. Samples should not be frozen except where specified, and transport conditions like freezing or formalin depend on the intended test.

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    © All Rights Reserved
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    11 views1 page

    Vetlab Info (1) : Samples For Laboratory Testing Against Avian Pathogens

    Uploaded by

    fasihaider
    This document provides guidance on sample collection and testing for avian pathogens at VetLab. It outlines which samples and sample types are needed for different types of laboratory tests, including serology, bacteriology, virus isolation, histopathology and molecular biology. For serology, a minimum of 0.5 ml of serum is needed, while various organs such as the heart, liver, spleen and joints can be sampled for bacteriology tests like Salmonella. Different virus diseases require samples from specific organs to be tested via virus isolation, histopathology, or PCR methods. Samples should not be frozen except where specified, and transport conditions like freezing or formalin depend on the intended test.

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    © All Rights Reserved
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    of 1

    VetLab Info (1)

    Samples for laboratory testing against avian pathogens


    1. Serology
    Paper strips

    Serum*

    ELISA
    Agglutination

    HI

    AGP

    SN

    * minimum 0,5 ml; for use of paper strips see VetLab Info 2

    2. Bacteriology
    Standard

    Heart

    Liver

    Spleen

    Caecum

    Ovaries

    Joints

    Salmonella

    Testing for other bacterial pathogens may require other sample material. Do not freeze samples.

    3. Virus isolation1), Histopathology2) , Molecular Biology3)


    Respiratory

    Reo

    IBD

    CAV

    Diseases

    Myco-

    Tumours

    plasma

    (MD, LL, REV)

    var. Organs, tumours

    nerves, proventriculus

    Liver

    + PCR

    + PCR

    Spleen
    Kidneys

    Trachea

    + PCR

    Feather Follicles
    Skin lesions

    + PCR

    PCR
    + PCR

    Bone marrow

    Pancreas

    PCR

    + PCR

    Joints

    Lungs

    ILT

    Bursa
    Caecal tonsils

    Pox

    PCR

    PCR

    1) indicates organs; to be transported frozen (-20C); 2) indicates organs; to be transported in 10% Formaline,3)
    PCR, no special transport requirements, swab as sampling material.

    Lohmann Tierzucht GmbH, Veterinary Laboratory, 21.12.10

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