PREGNANCY TEST

(FINAL

PROJECT)

Hussein Diab & Huy Nguyen

May 9th 2014

Material
Absorbent pad

Assay Strip

BSA
immobilization

Act as a fluid reservoir

porous sample receiver (505521, Filtrona
Fibertech)
(obtained from the ClearBlue pregnancy test)

Coffee filter paper

(Bovine Serum Albumin) on Nitrocellulose

Design
Width(in)

0.25

0.375

0.5

0.625

0.5

2 (s)

2.83

2.42

16.18

18.79

17.49

16.69

1.5

54.61

50.88

50.84

52.52

1 52.20

1 50.10

1 48.45

1 54.35

Distance(in)

We chose the size of the assay strip so that it was suitable to the absorbent pad.

Dimension for assay strip

Chemical
reaction time
Flow rate of the
liquid
The
concentration
Binding
efficiency

We need the
control line to
make sure the
test is complete,
but in our case
we dont need
the control line
due to some
resource
limitations.

The size of the assay strip does not affect the flow of the liquid. We just pick the dimensions so that it
is suitable to the absorbent pad.

Acquisition
We took pictures of the nitrocellulose paper with different concentrations

Each strip shows a different variation of Green

Calibration

Standard
curve
based on:

Blue
channel

Raw
(RGB)

Grayscale

Blue channel data

showed best distribution
with better regression for
more accurate results

Standard Curve

Conclusion
Complete
process
time

Less than 20
minutes

Limitation
of the
design

The nitrocellulose
is too dense so it
makes the liquid
take a lot of time
to get through

Improve
product

Pick different
types of
nitrocellulose to
make the process
fast

Every test strip can be used only once

Thank you