PRINCIPLE

p-nitrophenol-D-maltoheptaoside
(Amylase)
p-nitrophenylmaltotriose +
maltotetraose

Amylase
(AMS)

p-nitrophenylmaltotriose
(glucoamylase) pnitrophenylglycoside + glucose
nitrophenylglycoside
(glucosidase) p-Nitrophenol +
glucose

Lactate
Dehydrogena
se (LDH)

L- lactate + NAD (LD)

pyruvate + NADH + H+

Creatine Phosphate + ADP (CK &

Mg++) Creatine + ATP

Creatine
Kinase (CK)

ATP + D-glucose (HK) Glucose6-Phosphate + ADP

Glucose-6-Phosphate + NAD
(G6PD)
6-phosphogluconate + NADH + H+

CK - MB

Anti M antibody inhibits total

CK-MM and half CK MB

REAGENT

0.9 mM p-nitrophenyl-Dmaltoheptaoside
25,000 IU/L Glucosidase
(yeast)
10,000 IU/L Glucoamylase
(Rhizopus Sp.)
50mM NaCl
5mM CaCl2
50mM buffer
pH 6.9

75 mM L- lithium lactate
5.5 mM NAD
80 mM Buffer
pH 9

D-Glucose 20mM
Magnesium 10 mM
Adenosine-5-monoPO4
50mM
N-acetylcysteine 20 mM
Creatine PO4 30mM
Adenosine-5-diPO4 2mM
Oxidized NAD 2mM
G6PD 3000 U/L
Hexokinase - 3000 U/L
EDTA 2mM
Buffer 100mM

Creatine PO4 20mM

Adenosine-5-PO4 2mM
NAD 2 mM
Hexokinase (yeast) - >3000
U/L
G6PD (bacteria) - >2000
U/L
Anti-human CK-M antibody
(goat) sufficient amount to
inhibit up to 1500 U/L of CK-

SAMPLE
VOLUME

TOTAL
REACTION
VOLUME

WAVELENGT
H

LINEARITY

EXPECTED
VALUES

MULTIPLYIN
G FACTOR

STORAGE AND
STABILITY
Dry rgt @ 2-8C until
expiration date
Reconstituted rgt:
@15-30C 10 days
@2-8C 30 days

Serum: 0-96 IU/L

0.025 mL
(25 L)

0.025 mL
(25 L)

1.025 mL

1.025 mL

405nm

340 nm

1500 IU/L

Urine: 18-330
IU/L

4824

@ 30C
Male: 50-166 U/L
Female: 60-132
U/L
800 U/L

Serum and Urine

Amylase:
@18-25C 1 week
@2-8C several
months

Dry rgt @ 2-8C until

expiration date
6592

@37C
Male: 80-285 U/L
Female: 103
227 U/L

Reconstituted rgt:
@2-8C 14 days

Dry rgt @ 2-8C until

expiration date
6592
0.025 mL
(25 L)

1.025 mL

340 nm

1200 IU/L

@37C
25-192 IU/L
@30C
10-109 IU/L

0.050 mL
(50 L)

1.050 mL

340 nm

1500 IU/L

@37C
0-24 IU/L
@30C
0-14 IU/L
%CK-MB
<5.5%

If 3mL rgt +
0.1ml
sample,
4984

3376

Reconstituted rgt:
@ room temp. 24hrs.
@ 2-8C 21 days
Serum CK:
@ room temp. 4hrs.
@ 4C 8-12hrs.
@ frozen temp. - 2-3
days
Dry rgt @ 2-8C until
expiration date
Reconstituted rgt:
@ room temp. 8hrs.
@ 2-8C 14 days
Serum CK:
@ room temp. 24hrs.
@ 2-5C 14days
@ frozen temp. - 2-3

MM at 37C

PRINCIPLE

Gamma
Glutamyl
Transferase
(GGT)

Acid
Phosphatase
(ACP)

L-gamma-glutamyl-p-nitroanilide +
glycylglycine (GGT) pnitroaniline + gamma-glutamyl
glycylglycine

Alpha-naphtyhl + H20 (ACP)

Alpha-naphthol + inorg. PO4
Alpha-napthol + Fast TR Diazo
dye (chromophore)

REAGENT

GGT rgt reconstitution

Gamma-glutamyl-pnitroanilide 4.79mM
Glycylglycine 100mM
Tris 180mM @ pH 8.2
+0.1

6ml ACP rgt reconstitution

5ml L-tartate rgt recons.
Acetate buffer to acidify

days

SAMPLE
VOLUME

TOTAL
REACTION
VOLUME

WAVELENGT
H

LINEARITY

EXPECTED
VALUES

MULTIPLYIN
G FACTOR

STORAGE AND
STABILITY

Reconstituted rgt @28C: 14 days

0.050 mL
(50 L)

0.1 mL
(100 L)

1.050 mL

405nm

Male: 0-45 IU/L

Female: 0-30
IU/L

Total ACP:
0-9 IU/L

1.1 (total
ACP)
405nm
1.11 (nonpros ACP)

600 IU/L

60 IU/L at
37C

Prostatic ACP:
0-3 IU/L
Non-pros ACP:
0-6 IU/L

2121

Total ACP:
853
Non Prostatic
ACP:
860

Serum GGT:
@25C 8hrs
@2-8C 3 days
@-20C 1week