WEEK

TOPIC

Week 1

Meet- & -greet, First day of class Information.

Week 2

Week 3

Week 4

Week 5

Week 6

Week 7
March 19

March 26

General concepts and introduction to molecular genetics

- Phenotypic plasticity, as a source for natural selection, adaptation and evolution to take
place. Molecular genetic techniques can be used to study phenotypic plasticity from
several aspect of complexity.
Genes and proteins
- Genes structure and explanation of the central dogma of molecular
biology, that is, the flow of genetic information from DNA via RNA to polypeptide
(protein). What is a gene?
Basic molecular genetics techniques:
Restriction enzyme, southern blotting, DNA amplification (cloning and PCR) and
sequencing, are some very basic technique that will lay the foundation for the forthcoming
lectures.
DNA fingerprinting:
Different technique commonly used to fingerprint individuals as: a) RAPD, b) RFLP, c)
AFLP, d) RAD and e) microsatellite. Uses, advantages and disadvantages.
Hightrouput Next generation Sequencing (NGS) Technologies I - DNA sequencing:
How is changing the field of molecular genetics. Basic workflow. Comparison between
different platforms available: a) 454, b) Illumina, c) Solexa
Examples of application of NGS technologies to research studies.
Hightrouput Next generation Sequencing (NGS) Technologies II - RNA sequencing:
Transcriptome sequencing, and NGS gene expression analysis.
Bioinformatics challenges of NGS sequencing data:
How to work with very large amount of data. Genome assembling and gene prediction.
NGS and Target enrichment:
Enrichment of Exomes, amplicons and genomic region.
Gene expression profile, classical Microarray gene expression analysis:
Introduction to Microarray analysis and comparison to NGS - RNA seq
Microarray, Tiling array and Whole Transcriptome analysis/RNAseq
MIDTERM
&
Tour of Sequencing and Genotyping Facility (SGF):
Practical explanation of the setup of a genomic facility and NGS 454 workflow.
Services offered by the SGF (AFLP, Sequencing, 454, DNA & RNA quality and quantity
assessments).
PROJECT PRESENTATION
Introduction
Papers distribution
Student Assembly
Holy Week

Time
(hours)
3

Bosquejo de
contenido y
distribucin del
tiempo en horas