Concentration Dependency

of Precision
Horwitz et al. found a strikingly simple
exponential relationship between the
relative standard deviation among
laboratories (i.e. reproducibility) to the
concentration of the analyte, expressed
as a mass fraction

Precision for LC assay

System
Precisio
n

Concentrat
ion
Dependenc
y

a maximum permitted system precision is

defined, in relation to the upper specification
limit and the number of injections

Partly, a linear relationship was found in the

dependence of the variabilities from both
concentration fraction (Horwitz relation) and the
amount of analyte injected

The precision of the method was determined by

between-day and within-day studies, expressed
by the relative standard deviation (RSD) and
calculated using the double measurement of
peak area of each herbicide in the matrix. The
within-day precision RSDr was measured by
comparing standard deviation of the recovery
percentages spiked milk samples run the same
day. The between-day precision RSD was
determined by analyzing spiked milk samples
for four alternate days.