Penetapan Kadar Fenol Total

Cara Kerja
Hasil Percobaan :
a. Pembuatan Kurva Baku Asam Galat (=752 nm)
Kadar
(g/mL)
25
50
75
100
125

Absorbansi
0,224
0,484
0,707
0,720
1,039

Perhitungan regresi linier absorbansi (y) vs kadar (x) :

r = 0,9736
B = 7,464.10-3
A = 0,075
Y = BX + A
3

Y =7,464. 10 X +0,075
a. Sampel (=752 nm)
Stok sampel = 100 mg/10mL = 1000mg/100mL
Sampel

Absorbansi Kadar (g/mL)

I
II
III

0,959
0,915
0,926

Kadar (mg/100mL)
= Kandungan
Fenolik
11,8435
11,2540
11,4014

118,435
112,540
114,014

%b/b ekuivalen asam galat (EAG) = %b/b EAG

mg
)
100 mL
x 100
mg
Kadar stok sampel(
)
100 mL

Kandungan fenolik (
=

Sampel I

mg
)
100 mL
x 100
100
mg
0(
)
100 mL

11,8435 (
%b/b EAG =

%b/b EAG = 1,1844

Replikasi 1
mg
)
100 mL
x 100
mg
1000(
)
100 mL

11,2540 (
%b/b EAG =

%b/b EAG = 1,1254

Replikasi 2
mg
)
100 mL
x 100
mg
1000(
)
100 mL

11,4014 (
%b/b EAG =

%b/b EAG = 1,14014

1,1844 +1,1254+1,1401
3

%b/b EAG rata-rata =

=

1,14997

Susut Pengeringan
Macam

Sebelum Pemanasan

Setelah Pemanasan 5 jam

Bobot wadah tanpa ekstrak

Botol 1

Botol 1

Botol 2

Botol 2

(gram)

22,3504

Botol 1 (gram)

19,1478

22,3496

19,1469

Botol 2 (gram)

Bobot
wadah
+
ekstrak

Bobot
ekstrak

% bobot
konstan

Bobot
wadah
+
ekstrak

Bobot
ekstrak

% bobot
konstan

Sebelum
pemanasan

23,357
8

1,0082

20,1525 1,0056

Pemanasan I

23,133
3

0,7837

22,26

19,9290 0,7821

22,23

Pemanasan 2

23,132
4

0,7828

0,11

19,9280 0,7811

0,13

Senyawa sisa

Senyawa hilang

Botol 1

77,64 %

22,36 %

Botol 2

77,68 %

22,32 %