7.

Table of Experiment
N
O
1.

Procedure
Sample
0,5 mL of Saliva
- Diluted with graduated cylinder 50 mL

Experiment Result

Hypothesis/Reaction

Before:

The function of saliva as enzyme

Saliva :thick colorless

Amylum solution as substrate

Aquades: colorless
Enzyme solution
-Took 0,5 mL
100x
- Diluted with graduated cylinder 50 mL
- Took 0,5 mL
Enzyme solution
200x- Diluted with graduated cylinder 50 mL

After:
Saliva+diluted with
aquades: colorless

- Took 0,5 mL
- Diluted with graduated cylinder 50 mL
Enzyme solution
300x
- Took 0,5 mL
- Diluted with graduated cylinder 50 mL
Enzyme
solution
5 test tube test
400x
- Took 0,5 mL
- Diluted with graduated cylinder 50 mL
Enzyme solution
500x

2.

Change
The influence
of concentration with
the activity
of
enzyme
Result

Test Solution

Absorbance

Before:

The higher of enzyme concentration,

Amylum :colorless

the faster amylum that broken down

Conclusion

- Added 1 mL of amylum solution (solvent

aquades) 0,4 mg/mL
- Waited for 5 minutes

I2: orange

into simple molecules so that the

Aquades: colorless

lower levels of amylum.

After :
Amylum+waited

- Added 0,2 mL of sample

- Shake it
- Let it until 1 minute

5:colorless
Amylum+waited
0

- Added I2 0,01 N incubated until 60 C

- Tested UV-Vis with wavelength 680 nm

5+sample: colorless
After added by I2:
brownish yellow
After incubated: reddish
orange

3.

Before:

Test Tube Blanko

- Added 2 mL of amylum solution

(solvent aquades) 0,4 mg/mL
- Waited for 5 minutes

Amylum :colorless
I2: orange
Aquades: colorless

Change

- Added 1 mL I2 0,01 N and

incubated
-Added 8 mL of aquades

After :
Amylum+waited
5:colorless

Test Solution

- Tested UV-Vis with the wavelength

680 nm

Amylum+waited 5+ I2:
brownish yellow (+++)
After added by 8mL
aquades: brownish

Absorbance

yellow

4.

Effect of pH
5 test tube test
- Added 1 mL of amylum solution (solvent
pH 1, 3, 5, 7, 9)
- Let it until 5 minutes
- Added 4 drops of sample
Changes
- Added 5 mL of I2 0,01 N
- Added 8 mL of aquades
Test Solution
- Tested UV-Vis with the wavelength 680
nm

Before:

pH affects to the enzyme

Amylum: colorless

activation and enzyme

pH solvent: colorless

work actively at pH 7

sample: colorless
aquades : colorless
After :
Amylum+pH: colorless
Amylum+pH+waited5:
colorless
Amylum+pH+sample:
colorless
After added by I2:

Absorbance

brownish yellow (+++)

After added by aquades:
brownish yellow

1 test tube
- Added 1 mL of amylum
solution (solvent aquades)
- Let it for 5 minutes
- Added 1 mL of I2 0,01 N
- Added 8 mL of aquades

Amylum +let it
5:colorless
Amylum+let it 5 + I2:
brownish yellow
After added by aquades:

Test Solution
- Tested UV-Vis with the wavelength 680 nm

Absorbance

brownish yellow