Estudo de enzimas

para aplicao em Bio Fuel

Cell

ADH

Peso molecular:149.5 kDa

pH timo :5.4
Ponto isoeltrico : 6.91
Ativadores:

Reagentes ativadores sulfidricos

Mercaptoetanol
Ditiotreitol
Cisteina
Metais pesados
Agente quelante

Inibidores:

Metais pesados e reagentes -SH

Tiourea
Purina e derivados de pirimidina
Cloroetanol e fluoroetanol
Iodoacetamida
1,10-fenantrolina
8-hidroxquinolina
Analogos de Beta-NAD

GDH - glutamato desidrogenase

Desenvolvimento de BioFuel cell

Ocorrncia Natural
2-oxoglutarato + NH3 + NADH lutamato + NAD + H2O (glutamato
desidrogenase)

Desenvolvimento de BioFuel cell

GDH

GDH Esquema fuel cell ** ver

mediador usado

GOX
Peso Molecular: 160,000 Da
Ponto Isoeltrico : 4.2
pH timo : 5.5
Temperatura Otima : 40 C

Inhibitors:
Ag+, Hg2+, Cu2+

MalDH
Peso Molecular : 34 kDa
Ponto Isoeltrico : 4.9
pH timo :
9.0
Temperatura tima :
55
Inibidores: Ag+ Hg2 +

HRP
Peso Molecular : 40.000
Ponto isoelectrico : 7.2
pH timo : 7.0
Temperatura otima: 40 C

laccase
Peso Molecular = 62000 Da
Ponto Isoeltrico 2.6 7.6
pH timo = 3.0
Temperatura timo a 58 C.

LDH
Characteristics of LDH from Beef Heart:
Molecular Weight). 136,700

Specificity: The enzyme is specific for L(+)lactate. Meister

(1950) reports it reduces several -keto and ,-diketo acids
but at about one-tenth the rate of reduction of pyruvate.
Inhibitors: LDH is quite stable. It is inactivated by iodide.
Inhibition by p-mercuribenzoate is slow. See Schwert and Winer
(1963); also Andersonet al. (1974) and Bloxhamet al. (1975).
Activators: A number of organic compounds which stabilize
the enzyme, such as dimethyl sulfoxide, ethanol, and
methanol, are reported by Georgeet al. (1969).
Diethylstilbestrol and several of its derivatives also stabilize
the enzyme (Cohenet al. 1969).

tabela
Tamanho
N sitios
Inibidores
Ativadores
Temp.
pH
PI
substrato

*Nafion pKa, fornecedor