The Effect of BPA

on Healthy
Neuron cells
By: LOGAN BIRCHMORE

Purpose
To see if exposure to BPA can cause major
health problems.
To test if the FDA was right when it said BPA
was safest at its lowest concentration which
occurs in food.
The purpose of this lab was to figure out how
BPA effects healthy neuron cells.

EDD
The effect of BPA on healthy neuron cells.
Hypothesis

I believe the higher concentration of BPA will cause more of the

healthy neuron cells to die.

IV

The amount of BPA.

Constants

Amount of time in incubator.

Trials

4 trials. Control. Low. Medium. High.

DV

How many cells die

Overview/Procedure
s
I will place cells in 16 different wells.
I will use a pipette to apply growth medium,
I will then turn the cells upside down then right side up slowly and carefully to make sure the cells do
not stick to the bottom.
I will then apply the different concentrations of BPA into the four wells for each concentration.
I will incubate the cells for 24 hours.
I will then place the cells in a hemocytometer and apply trypan blue.
I will then place the cells under the microscope and count the number of cells that are still alive.
I will then record what I saw.

Results discussion
125

100

Trial 1

Trial 2

Trial 3

Trial 4

75

50

Mean

110.5

102

100

101

25

Median

54

53.5

51.5

54

Mode

N/A

N/A

N/A

N/A

Control

Low
Trial 1
Trial 4

Medium
Trial 2

High

Range

Trial 3

Standard 39.16950 41.35214 38.87587 37.35862

Deviation 344
626
084
239

Conclusion
My hypothesis was supported.

My hypothesis was
the higher the concentration of PBA the more
healthy neuron cells that will die. The highest
concentration had below 15 and less cells still alive,
while the lowest concentration of PBA has 78 and
more cells still alive.
Although some sources of error could be some of the
cells died off before having the PBA applied to the
cells.
The cells could have doubled(reproduce).
For future research I can test PBA on different types
of cells to see if it effects the body drastically by
killing off other cells.