Global

Local at promoters of genes

Same
nucleus

Differential
ChIP
experiments

Taverna et al. 2007

chromodomain

chromodomain

Dominant negative

Taverna et al. 2007

Histone/chromatin crosstalk

appears in mitosis but not interphase.

appears in mitosis but not interphase.

Chromatin / Nucleosome dynamics

Nucleosome folding /packaging regulates
accessibility of DNA templates (nucleosomal DNA and
linker DNA)
The dynamics of chromatin structure are regulated
through multiple mechanisms including:
--histone variant incorporation
--histone posttranslational modifications (PTMs)
--ATP-dependent chromatin remodeling
--histone chaperones
--architectural chromosomal proteins

**

Cell cycle regulated

Tessarz and Kouzarides (2014)

Results

Conclusions

Jack et al (2013)

Lysine

Tessarz and Kouzarides (2014)

Tessarz and Kouzarides (2014)

PTMs may affect

nucleosome structures and
dynamics

Nucleosome lateral surface

modifications
-may modulate histone-DNA
interactions --> nuc stability -> nuc position stability ->
increased mobility =
increased accessibility

Tessarz and Kouzarides (2014)

Nucleosome
histone-histone
modifications
--may destabilize
tetramer-dimer
interactions --> nuc
stability --> histone
exchange

Tessarz and Kouzarides (2014)

Nucleosome face
modifications
--may modulate
chromatin-binding
protein interactions
positively / negatively
--may modulate nucnuc interactions -->
higher order chromatin
structure

+ respective writers, erasers, readers

+

EFFECTORS (dynamic interactions)