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Cell Dyn 3500
Cell Dyn 3500
TABLE of CONTENTS
INTRODUCTION
DATA
RS-232 TRANSPORT
INTERFACE SPECIFICATION
CELL-DYN 3500 SYSTEM
includes Reticulocyte Parameters
Abbott Laboratories
Abbott Park, IL 60064
Table of Contents
1.0
INTRODUCTION.................................................................................................... 1
1.1 Purpose..................................................................................................... 1
1.2 Definitions and Conventions...................................................................... 1
1.3 Scope........................................................................................................ 1
2.0
DATA ..................................................................................................................... 1
2.1 General Message Form............................................................................. 1
2.2 Instrument Messages ................................................................................ 2
2.3 Host Messages ....................................................................................... 19
3.0
NOTES
ii
1.0
INTRODUCTION
1.1
Purpose
This document describes the communication methods and interface characteristics of
Abbott CELL-DYN 3500 automated hematology analyzer when connected to a Host.
1.2
1.3
Scope
This document is divided into two major sections, DATA and RS-232 TRANSPORT.
This DATA section describes 2 communication protocols: one for transmitting data while in
Hematology mode and one for transmitting data while in Retic mode.
The RS-232 TRANSPORT section describes the means by which the Instrument and
Host transfer data to each other.
2.0
DATA
This section describes the data that is transferred from the Host to the Instrument and
from the Instrument to the Host. The syntax and meaning of communication messages is
described without reference to the underlying transport mechanism.
2.1
2.1.4.2
2.1.4.3
Text fields begin and end in double quotation marks (") and use
the blank character to occupy empty positions. RTC message
Type floating point values are transmitted as text.
2.1.4.4
2.1.4.5
2.1.4.6
Empty fields
2.1.4.7
2.1.4.8
2.2
2.1.4.6.1
2.1.4.6.2
Spare Fields
2.1.4.7.1
2.1.4.7.2
Instrument Messages
Instrument messages are transmitted by the Instrument to the Host. All of these contain
result data. Each message comprises an identification segment followed by a results
segment.
2.2.1
2.2.2
2.2.1.2
2.2.1.3
2.2.2.2
"WB1"
"WB2"
"RBC"
"PLT"
"WIC"
"
"RTC"
" (3 blanks)
2.2.2.3
2.2.2.4
2.2.2.5
Specimen ID Field 5
The specimen ID is the fifth field in the Identification Segment. For
patient samples, the specimen ID is entered by the operator on the
Instrument, while for other samples, such as controls and
background samples, the specimen ID is supplied by the
Instrument. In either case, it occupies a text field of twelve (12)
characters plus the enclosing double quotation marks.
[Start of text]
"WB1"
[message type]
0416,
[sequence no.]
0,
0,
[specimen type]
"------------"
[specimen ID]
"----------------"
[specimen name]
"stc",
[operator ID]
"01/25/89",
[specimen date]
"14:08",
[specimen time]
00000,
[scale factor]
000,
[channel 2 data]
000,
[channel 6 data]
000,
[channel 10 data]
018,
[channel 14 data]
024,
[channel 18 data]
052,
[channel 22 data]
096,
[channel 26 data]
112,
[channel 30 data]
098,
[channel 34 data]
070,
[channel 38 data]
051,
[channel 42 data]
038,
[channel 46 data]
033,
[channel 50 data]
029,
[channel 54 data]
023,
[channel 58 data]
024,
[channel 62 data]
023,
[channel 66 data]
019,
[channel 70 data]
018,
[channel 74 data]
021,
[channel 78 data]
022,
[channel 82 data]
025,
[channel 86 data]
024,
[channel 90 data]
025,
[channel 94 data]
032,
[channel 98 data]
033,
034,
036,
033,
033,
042,
054,
058,
056,
065,
072,
074,
077,
081,
085,
085,
080,
066,
059,
054,
043,
042,
037,
026,
022,
020,
016,
010,
006,
006,
005,
002,
001,
002,
002,
001,
001,
001,
000,
F7
[checksum]
ETX
[end of text]
2.2.3.2
2.2.3.2.2
2.2.3.2.3
2.2.3.2.4
2.2.3.2.5
2.2.3.2.6
2.2.3.2.7
2.2.3.2.8
2.2.3.2.9
10
11
12
[Start of text]
" ",
[message type]
0416,
[sequence no.]
0,
0,
[specimen type]
"------------",
[specimen ID]
"-----------------",
[specimen name]
"stc",
[operator ID]
"01/25/95",
[specimen date]
"14:08",
[specimen time]
05.00,
[WBC count]
03.20,
[NEU count]
01.20,
[LYM count]
0.300,
[MONO count]
0.200,
[EOS count]
0.100,
[BASO count]
04.94,
[RBC count]
015.3,
[HGB value]
043.1,
[HCT value]
092.3,
[MCV value]
031.0,
[MCH value]
034.4,
[MCHC value]
012.0,
[RDW value]
00177,
[PLT count]
010.7,
[MPV value]
01.90,
[PCT value]
019.2,
[PDW value]
064.0,
[NEU % value]
024.0,
[LYM % value]
06.00,
[MONO % value]
04.00,
[EOS % value]
13
14
02.00,
[BASO % value]
1,
1,
[DFLT flag]
0,
[Blast flag]
0,
1,
1,
0,
0,
[IG flag]
0,
[Band flag]
0,
0,
0,
[IG/Bands flag]
0,
[FWBC flag]
1,
0,
0,
[DLTA flag]
0,
[NWBC flag]
1,
0,
[RRBC flag]
0,
[spare flag]
0,
0,
[spare flag]
1,
[LRI flag]
0,
[URI flag]
07761,
02473,
00000,
00000,
02445,
01223,
00000,
[spare field]
00000,
[spare field]
05.00,
04.80,
3,
[limits set]
1,
[sample mode]
0,
1,
0,
F7
[checksum]
ETX
[end of text]
Patient type
1:
QC sample type
2:
Background type
15
17
2.2.5.3
18
Field 29
[Spare Flag]
Field 30
[Spare Flag]
Field 31
[Spare Flag]
Field 32
[Spare Flag]
[Start of text]
"RTC",
[message type]
0416,
[sequence no.]
0,
0,
[specimen type]
"------------",
[specimen ID]
"----------------",
[specimen name]
"M",
[specimen sex]
"10/25/75",
[specimen DOB]
"----------------------"
[Dr name]
"stc",
[operator ID]
"01/25/95",
[specimen date]
"14:08",
[specimen time]
"1.000",
[Retic%]
"50.00",
[Retic Abs]
"5.000",
[RBC count]
"0.000",
[Background count]
"0.010",
"0.000",
[spare field]
"0.000",
[spare field]
"0.000",
[spare field]
"0.000",
[spare field]
"0.000",
[spare field]
"0.000",
[spare field]
1,
[Flow error]
0,
[Fragile RBCs]
0,
0,
[ENC]
0,
[ERL]
0,
0,
[spare flag]
0,
[spare flag]
0,
[spare flag]
0,
[spare flag]
F7
[checksum]
ETX
[end of text]
2.3
Host Messages
Host messages are transmitted by the Host to the Instrument. The Instrument uses Host
messages to add entries to the work list. However, if a Host message contains syntax or
functional errors, the work list is not altered.
2.3.1 Instrument Reception Mode
To prepare the Instrument to receive work list messages from the Host, the
operator should turn the bar code option on in the Work List menu, then go to the
Work List Set Up menu and choose the desired association for the bar code ID
(4-digit CELL-DYN 3500 System bar code or Laboratory Specimen ID) When the
bar code option is off, the Instrument considers all Host messages to contain
syntax errors.
19
20
[Start of text]
"1135",
"237-45-7789",
[specimen ID]
[specimen Name]
0,
[limit set]
3,
[parameter set]
F7
[checksum]
ETX
[end of text]
1
[Start of text]
"1135",
"237-45-7789,"
[specimen ID]
[specimen Name]
0,
[limit set]
3,
[parameter set]
"Jones, Henry
",
[doctor name]
"09/30/45",
[date of birth]
F7
[checksum]
ETX
[end of text]
1
21
3.0
RS-232 TRANSPORT
3.1
Mechanical Interface
The Instrument provides a standard DB9 male connector mounted on the back
panel. The RS-232C interface is configured as DTE (Data Terminal Equipment). A
cable is available for converting the 9-pin interface to a standard 25-pin interface.
Pins on the DB9 connector:
Pin 1:
Pin 2:
Pin 3:
Pin 4:
Pin 5:
Signal Ground
Pin 6:
Pin 7:
Pin 8:
Pin 9:
Further information on the physical interface can be obtained from the IBM Technical
Reference, Options and Adapters (Copyright IBM Corporation).
3.2
Electrical Interface
Voltage levels and electrical characteristics are defined by the EIA RS-232C
specification. The maximum recommended cable length is 30 meters, or 100 feet.
The maximum workable cable length may differ from that figure because of
environmental factors, the selected baud rate, and the nature of the equipment being
connected.
3.3
Data Link
3.3.1
Symmetrical Interface
The communication is full duplex; the Host and the Instrument may begin
transmitting at any time except as restricted by error control and recovery.
3.3.2
Asynchronous Serial
The transmission of data by the Instrument is in an asynchronous, bit-serial
format, using the ASCII character set. A setup menu is provided for specifying
the number of bits per character (seven or eight), the number of stop bits (one
or two), the parity (even, odd, or none), and the baud rate (300, 600, 1200,
2400, 4800, or 9600).
22
23
24