BC-2800 Service Training: Hematology Global Technical Support Department Version V2.0 NO.:MXQ-12028-BC-2800

BC-2800 Service Training
Hematology Global Technical Support Department

Service@mindray.com
Version ： V2.0
NO.:MXQ-12028-BC-2800
© 2014 Mindray Confidential
OTY-12002 （ 3.0 ）
Contents
1.1 Basic Knowledge

1.2 Principle
2.1 Installation
2.2 Operation
2.3 Liquid System
2.4 Hardware
2.5 System Structure
2.6Trouble Shooting
2.7 Maintenance
2 © 2013 Mindray Confidential
1. Principle
1.1 Basic Knowledge
1.2 Principle

1.1 Basic Knowledge
Blood Cell Leukocyte Erythrocyte Platelet
Blood
 Blood sample consists of two parts.

1.1 Basic Knowledge
Blood Leukocyte Erythrocyte Platelet
Blood Cell
 There are three kinds of blood cells.
Leukocyte Erythrocyte Platelet

Size: 30-400fl 30-150fl 2-25fl
9 12 9
Amount: 4-10X10 /L 3.5-5.5X10 /L 100-300X10 /L
1.1 Basic Knowledge
Blood Blood Cell Erythrocyte Platelet
Leukocyte
Granulocyte
WBC
Defend the body
against infectious
disease and foreign
materials

1.1 Basic Knowledge
Blood Blood Cell Erythrocyte Platelet
Leukocyte
Lymph Baso Mono Eosino

Neutro
Lymphocyte Mid-sized Cell Neutrophilic Granulocyte

1.1 Basic Knowledge
Blood Blood Cell Leukocyte Platelet
Erythrocyte
 The function of RBC is almost completed by hemoglobin (HGB).
Transport oxygen and carbon dioxide

1.1 Basic Knowledge
Blood Blood Cell Leukocyte Erythrocyte
Platelet
Coagulation or clotting

1. Principle
1.1 Basic Knowledge
1.2 Principle

1.2 Principle
Electrical Impedance Volumetric Metering Colorimetric Method Reagents
Method
 WBC, RBC and PLT are counted and sorted by the Electrical Impedanc
e Method, just Coulter Principle. This method is based on the measurement
of changes in electrical impedance produced by a particle passing through a
n aperture.

1.2 Principle
Method

1.2 Principle
Method
The amplitude of each pulse is proportional to the size of each blood cell.
1.2 Principle
Method
Pulse Graph
1 、 blood cell of Same size, go through the
aperture by constant speed.
2 、 blood cell of Same size, go through the

aperture by variable speed.
3 、 blood cell of different size, go through

the aperture by variable speed.

1.2 Principle
Method

1.2 Principle
Electrical Impedance Reagents
Volumetric Metering Colorimetric Method
Method
 From Pulse Graph to Histogram

1.2 Principle
Method
From Pulse Graph to Histogram

1.2 Principle
Electrical Impedance
Volumetric Metering Colorimetric Method Reagents
Method
 This analyzer uses a volumetric metering unit to control the count cy

cle and to ensure that a precise volume of sample is analyzed.
Uppersensor Uppersensor
Lowersensor Lowersensor
1Emptywhenstart 2Liquidsurfacefalldownthroughthe
volumeritctube
Uppersensor Uppersensor
Lowersensor Lowersensor
3Countingstartwhenthe 4Countingendwhentheliquid
liquidsurfacepasstheupper surfacepassthelowersensor
sensor.

1.2 Principle
Method
 HGB is determined by the colorimetric method.

 Lyse will release HGB when RBC was broken down and react with
HGB to generate a mixture for HGB measurement.
 The mixture has the biggest absorbance at 540nm.
I0 It

1.2 Principle
Method
Absorbing Detector Signal

Light
container processing
source
Display
Photo
Bath sensor
LED light

1.2 Principle
Electrical Impedance Colorimetric Method
Volumetric Metering
Method Reagents
 Reagents are one part of the system.
 Lyse
Break down RBC;
Release HGB and react with HGB to generate a mixture for HGB m
easurement;
Help the differential of WBC.
 Diluent
Dilute the blood sample;
Provide a conductive environment for counting and an environment
which is similar to the plasma to maintain the size of blood cell;
Clean the tube and bath.

1.2 Principle
Volumetric Metering Colorimetric Method
Method Reagents
 Rinse
Clean the bath and metering tube.
 E-Z cleanser
An enzyme-based cleaning solution used to clean the tube and bat
h.
 Probe cleanser
An alkaline cleaning solution used to clean the tube and bath.

2.1 BC2800 Installation
Installation Requirements
2.1.1 Interface
2.1.2 Installation Requirements
Open box, check 2.1.3 Installation Procedures
Install
Performance validation

2.1.1 Interface- Front Panel
8.4” color LCD
Keypad
Record
Power indicator
Sample
probe
Start key

2.1.1 Interface- Left Side
Scanner interface
Printer or upgrade interface
Transmission to computer
Power of floppy disk driver
Keyboard
Printer:
1.EPSON LX-300/300+ ,
2. EPSON LQ-300K+II, LQ630K
3. EPSON Stylus C-87 , Panasonic KX-
P1150
Scanner: TYSSO CCD-82, AIDA8400
2.1.1 Interface- Rear Panel
Diluent connector
Rinse connector
Power Switch
Lyse connector
Equipotent Pole Waste connector
Fuse
Environment Space Power
 Operating temperature: 15 ℃ to 30 ℃.
 Relative humidity: 30% to 85%.
 Atmospheric pressure: 70 kPa to 106 kPa.
 free as possible from dust, mechanical vibrations, loud noises, a
nd electrical interference.
 Do not place near brush-type motors, flickering fluorescent light
s, and electrical contacts that regularly open and close.
 Do not place in direct sunlight or in front of a source of heat or dr
afts.

 At least 40 cm on each side, which is the preferred

access to perform service procedures
 At least 10 cm behind for cabling and ventilation
 There is enough room on or below the countertop to

accommodate the diluent, rinse and waste containers.
(reagents bottle better be the same level of the machine).


 Check the availability of a power outlet that is

 a female receptacle;
 single phase with ground (confirmed third-wire earth ground);
 voltage of zero curve to ground should be ≤5V
 voltage: 100 VAC - 240 VAC;
 frequency: 50/60±1 Hz
 fuse: 250V T4A
 Better to have an UPS: 500W
 Properly grounded socket or connect the grounding pole at the
back of the analyzer to the ground.

2.1.3 Installation Procedures
Release the sample probe Step2 Step3 Step4
1. Remove the foam

2. Move the sample probe to the middle position

Step1 Reagents connection Step3 Step4

Step1 Step2 Install Recorder Paper Step4
1.Open the door of the recorder.

2.Insert the paper into the paper entry.
3.Push the bar of the recorder.
4.Close the door.
Note: The thermo-side should faces up.

Step1 Step2 Step3 Performance Validation
Start the machine
Check background
Do QC test,
check repeatability
Test fresh blood sample
Any step has problem,

please check the machine

2.2 BC-2800 Operation
N
Repeatability good
2.2.1 Count Screen
Y Y N
2.2.2 Menu Tree
Calibration Troubleshooting
2.2.3 Start up
2.2.4 Quality control
2.2.5 Sample and dispense
2.2.6 Linear and Display Range
2.2.7 Parameters

2.2.1 Count Screen
System System
Title Count Mode Status time
Window
Error Messages Reagent Status

MENU On-line Help

Count
2.2.2 Menu Tree Revi ew
Qual i t y cont r ol Cont r ol s
X- B anal ys i s
Set up Pas s wor d
Set t i ngs
Ser vi ce Mai nt enance
St at us
Sel f - t es t
Log
Conf i g
Pr i nt
Cal i br at i on Manual
Aut o
Aut o - f r es h bl ood
Shut down

2.2.3 Startup
 After powering on the instrument, the power indicator will light on an

d the screen will display “Initializing…”.
 The analyzer will sequentially initialize the file, hardware and fluidic
systems.
 The whole initializing process lasts about 3 - 5 minutes, depending o
n how the analyzer was previously shut down.
Parameter Background Range

 Background Test WBC  0.3  109 / L
RBC  0.03  1012 / L
HGB 1g/L
HCT  0.5 %
PLT  10  109 / L

2.2.4 Quality Control
 Daily work: Before running any samples, use Quality Control or Fre
sh Blood to check the stability of instrument.

 Check CV ： Run 11 tests and delete the first result, oth

er 10 results for analyzing.
n Parameter Linear Repeatability (CV%)

x i 4.0-7.5×109/L ≤ 3.0
Mean＝ i 1 WBC
7.6-15.0×109/L ≤ 2.0
n
3.0-4.5×1012/L ≤ 2.0
RBC
SD 
X i  Mean 
2
4.5-6.5×1012/ L ≤ 1.5
HGB 110 – 180g/L ≤ 1.5
n 1
MCV 70.0 – 100.0fL ≤ 1.0
SD 100-200 ×109/L ≤ 5.0
CV%   100 PLT
201 - 500×109/L ≤ 4.0
Mean

 Good repetition, poor accuracy
Recalibrate
 8.9, 9.0, 8.9, 8.9, 8.8 (reference value: 9.6+0.5)
 Poor repetition, poor accuracy Troublesho

 8.5, 9.4, 10.1, 8.0, 10.5 oting
 Good repetition, good accuracy Perfect

 9.6, 9.7, 9.7, 9.6, 9.5
2.2.5 Sample and dispense
 Compare
Blood Lyse
 Whole Blood mode 13ul 0.5ml

Prediluted mode 20ul 0.36ml
 Liquid tested Diameter of aperture

WBC/HGB 300ul 100um
RBC/PLT 300ul 70um
 Ratio of dilution Whole blood Capillary blood

WBC/HGB 1:308 1:400
RBC/PLT 1:44862 1:44402
 Aperture Size: 80um （ diameter ）， 70um （ depth ）
 Count time ： less than 2 min / test

2.2.6 Linear and Display Range
Parameter Linear Display

WBC 0 - 99.9 ×109/L 0.0-499.9 ×109/L
RBC 0 -9.99×1012/L 0.0-9.99 ×1012/L

HGB 0 - 300g/L 0- 300g/L
MCV ------ 0-250 fL
PLT 0 - 999×109/L 0-2999 ×109/L

2.2.7 Parameters
Histogram Calculation Alarm
From Measurement
BC-2600can give out 20 parameters and 3 histograms

 Results coming from measurement
White Blood Cell WBC Red Blood Cell RBC

Lymphocyte Lymph# Hemoglobin HGB
Mid-sized cell Mid# Hematocrit HCT
Granulocyte Gran# Mean Cell (erythrocyte) Volume MCV
Lymphocyte % Lymph% Mean Cell (erythrocyte) Hemoglobin MCH
Mid-sized cell% Mid% Mean Cell (erythrocyte) Hemoglobin
Granulocyte% Gran% Concentration MCHC
Coefficient of variation RDW-CV
Platelet PLT Red Blood Cell (erythrocyte) Distribution
Mean Platelet Volume MPV Width Standard deviation RDW-SD
Platelet Distribution
Width PDW
Plateletcrit PCT
Large Platelet ratio P-LCR
2.2.7 Parameters
Measurement Calculation Alarm
From Histogram
BC-2600 can give out 20 parameters and 3 histograms

 Results derived from the histogram
Width PDW
Plateletcrit PCT
Platelet ratio P-LCR
2.2.7 Parameters
Measurement Histogram From Calculation Alarm
BC-2600 can give out 20 parameters and 3 histograms

 Results calculated by formula
Width PDW
Plateletcrit PCT
Platelet ratio P-LCR
2.2.7 Parameters
Measurement Histogram Calculation
Parameters Alarm
 Alarm
“H” or “L” is indicating the data has exceeded the setup higher or lo
wer alarm limit of the parameter.

If the result is “***”, it indicates that the data is unreliable or has exce
eded the higher limit of the display range.

9
If the result of WBC is less than 0.5  10 /L, the system will not perfo
rm leukocyte differential and shows “***”.

2.2.7 Parameters
 Alarm
WBC differential alarm
R1 Alarm
R2 Alarm
R3 Alarm
R4 Alarm
Rm Alarm
PLT alarm
Pm Alarm
PS Alarm
PL Alarm
2.3 BC-2800 Hydraulic System
2.3.1 Components
2.3.2 Hydraulic Diagram
2.3.3 Counting circle

2.3.1Components
Includes ： 11 Valves(7 3-ways valves, 4 2-way valves) ，

aperture,
sample probe,
Syringe: 7.5mL & 50uL long life
Aperture : Ø80um
pump, count bath, volumetric tube, wipe block,
vacuum/pressure chamber, tubing…

Valves
Includes ： 11 Valves(7 3-ways valves, 4 2-way valves)
When power on, When power on,

switch open
• Three way valve Two way valve

Sample probe & bath
• Sample probe
Sample
• Rotatory motor probe
• Fluctuating motor assembly
• Counting bath
• HGB assembly
Counting
cell & HGB
unit

Syringe & motor
 7.5mL syringe
 50uL syringe Syringe assembly
 Syringe sensor
 Syringe motor

2.3.1Components
 Vacuum chamber
 Waste pump

2.3.2 Hydraulic Diagram

2.3.4 Counting circle
Sample
Lyse
Diluent
Rinse
Waste
Air

Aspirate sample (whole blood)

Sample probe cleaning and bath cleaning
Read HGB Blank

Add sample, Draw sample(RBC)

Aspirate Lyse, Add lyse ,Establish pressur
e ,Bubble mixing

WBC Counting
Read HGB Sample

Clean bath

Add sample(RBC)

Establish pressure ,Bubble mixing, RBC Count
ing

Clean bath

Aspirate sample (pre-diluted)

Sample probe cleaning and bath cleaning
Read HGB Blank

Add sample, Draw sample(RBC)

Aspirate Lyse, Add lyse ,Establish pressur
e ,Bubble mixing

WBC Counting
Read HGB Sample

Clean bath

Add sample(RBC)

Establish pressure ,Bubble mixing, RBC Counti
ng

Clean bath

Clean-flush

2.4 BC-2800 Hardware
2.4.1 Hardware Diagram

2.4.2 Board Structure
2.4.3 CPU board
2.4.4 Power supply board
2.4.5 Power driver board
2.4.6 Analog board
2.4.7 Volumetricboard

2.4.1 Hardware Diagram
Old
version

2.4.2 Board Structure

2.4.3 CPU board

2.4.3 CPU board
Power converter Part on CPU board

Main function—CPU board
1. Receive signal from Analog board

WBC & RBC/PLT count signal
HGB signal
Aperture voltage
Pressure/vacuum
2. Monitor
+48V 、 +12V 、 -12V from analog boa
rd
+3.3V 、 +12V from CPU board
Temperature
3. Control
Keypad
Valves & pump
HGB lamp
Constant current
Potentiometer
4. Drive
LCD display
Keyboard
Printer
Floppy disk driver

2.4.4 Power Supply board
 Input power 100~240V ，

 Output 5V 、 12.8V 、 30V 、 ±12V
 3 power lines to CPU board, analog signal
board and power driver board. CPU board
supplies power to other boards.
 Old version 2800:A transformer converts AC input
power to AC53V(converted to aperture constant
current 48VDC) and AC120V(ZAP function)
 New version: these circuit are on analog board

2.4.5 Power driver board
Input power from power supply board (12V 、 30V)
 The driving board drives the valves, pumps and motors.

 It carries out the following instructions sent by the CPU:
to open/close the pumps or solenoid valves;
to control the motors of the syringes;
to control the movement of the sample probe;
to remain the torques of the motors when the analyzer
has entered the screen saver.

2.4.6 Analog signal board
Power board supplies ±12V

Old version Basic functions:
 Power supply unit:
 Volumetric signal adjustment unit;
 HGB signals adjustment unit:
 Vacuum/pressure signals adjustment unit:
New version
 Monitor unit: monitor operating voltage and the vol
umetric signals sensor.
 Interface unit: interface of analog board and CPU
board.

2.4.7 Volumetric board
Control the count cycle. Each count cycle starts

when the liquid surface reaches the upper sens
or and stops when the surface passes the lower
sensor.

2.5 System Structure

Overview

Removing the left, right and top covers

Removing the front cover

Disassemble boards

Syringe

Counting Bath
Note: The concave side of the aperture must face to

the front bath.

Counting bath

3-way valve

3-way valve

2.6 Trouble shooting
2.6.1 Adjust Gain
2.6.2 Histogram and pulse graph
2.6.3 Trouble shooting

2.6.1 Adjust Gain

General
Adjust the Gain of the Channel in the Following Situations:
 Replace count bath
 Replace aperture
 Replace analog signal board, CPU board, DOM
WBC (whole blood), WBC (pre-diluted), RBC, HGB and PL

T

Gain of WBC Channel
 too small WBC gain that should be increased
 too large WBC gain that should be decreased

Gain of WBC Channel
 Use normal volunteers’ fresh EDTA-K2 anticoagulant

venous blood;
 Make the end position of histograms of most blood
samples between 320-350fl;
 Set up WBC (pre-diluted) gain the same as the WBC
(whole blood) gain.

Gain of RBC/PLT Channel
 When the difference between the actual MCV anal

ysis result and the expected result exceeds 6%, ne
ed to change the RBC gain.
 For example, assuming the expected MCV result i
s 90.0fL, while the actual analysis result is 82.0fL, t
he gain factor as below:
ExpectedMCV 90.0
＝  1.098
ActualMCV 82.0

Gain of HGB Channel
• Modify the background voltage value of HGB

within the range of 3.4-4.8V (4.5V recommended).

2.6.2 Histogram and pulse graph

Normal histogram
Fluctuating
Discriminator WBC 2 3 4
1
1 30~85fL
2, 3 85~125fL
Fixed Discriminator
4 400fL
[fL]
Lymph Mid Neutro
(Baso 、 Mono 、 Eosino)

No differential result
No differential result because the WBC histogram is o

ver-narrowly compressed.

No differential result because WBC count result is les

s than a certain value (WBC < 0.5)

No differential result because the peak of WBC histogram lies i

n the middle of the histogram and thus cannot identify the type
of peak cells

Increased nucleated erythrocytes or
interference or inadequate lyse

No lyse reagent or poor lyse

Severe interference in WBC channel

Increased lymphocytes

Increased mid-sized cells

Increased neutrophilic granulocytes

Pulse Graph
 After each count, the system can save the origina
l sampling pulses of this time. We can analyze th
e reason leading to the fault by viewing these orig
inal data.
 For BC2800, please press F5 to view WBC pulse

and press F1 to switch to RBC and PLT pulse gra
ph.

WBC pulse Graph of normal sample

WBC pulse graph of normal background

RBC pulse graph of normal sample

RBC pulse graph of normal background

PLT pulse graph of normal sample

PLT pulse graph of normal background

Severe interference in WBC channel (background)

Severe interference in WBC channel (sample)

Severe interference in RBC channel (background)

Severe interference in RBC channel (sample)

Severe interference in PLT channel (background)

Severe interference in PLT channel (sample)

Interference occurs because gain of PLT channel is
too large

Slight interference in WBC channel

Inadequate or no hemolysis in WBC channel

Slight interference in RBC channel

Slight interference in PLT channel

Interference in WBC channel caused by inverter
Feature: sine wave

Interference in WBC bath from inverter

Insufficient liquid in WBC bath during count

Interference in RBC channel from tubing

Insufficient liquid in RBC bath during count

Interference in PLT channel from tubing

Insufficient liquid in PLT bath during count

2.6.3 Troubleshooting

Background abnormal
Background
abnormal  Not well grounded
 Shielding cover of bath and analog board
WBC(RBC) clog
 Electrical interference
WBC(RBC) bubble  Reagents dirty
Vacuum low  Bath, valve or tube dirty
 Wipe block, diluent dispenser, shielding cover,
Pressure low
 7.5mL syringe dirty
Results abnormal  Plastic washer dirty, aging
WBC abnormal  Replace bath
 Replace analog board
Syringe error
 Pulse graph
Display problem
No start up

WBC(RBC) Clog
Background
abnormal
 Reagents empty
WBC(RBC) clog
 Diluent added into the bath sufficiently
WBC(RBC) bubble  Zap, flush
Vacuum low  Aperture voltage: 9~14V, if high, unclog
 Watch volumetric board
Pressure low
 Fluid pass normal, volumetric board defective or need adjustmen
Results abnormal t, CPU board
 Fluid pass abnormal, check valve 6, tube between valve 6 and ba
WBC abnormal
th, valve 6 to volumetric tube
Syringe error
Display problem
No start up

WBC(RBC) Bubble
Background
abnormal  Reagents empty
WBC(RBC) clog  Diluent prime, rinse prime
 Diluent added into the bath sufficiently
WBC(RBC) bubble
 Watch volumetric board
Vacuum low  Fluid pass normal, volumetric board defective or need adjustm
Pressure low ent, CPU board

 Fluid pass abnormal, check valve 6, tube between bath to vac
Results abnormal
uum chamber leak or not.
WBC abnormal
Syringe error
Display problem
No start up

Vacuum low
Background
abnormal  Service->System status->Vacuum
 Vacuum filter
WBC(RBC) clog
 Waste container full
WBC(RBC) bubble  Waste tube bent
Vacuum low  Tube connections between valve(6,7,8,9,10)
 Valve(6,7,8,9,10)
Pressure low
 Waste pump block, clean
Results abnormal  Vacuum chamber leak
WBC abnormal  Tube between vacuum chamber and analog board
 Analog board
Syringe error
Display problem
No start up

Pressure low
Background
abnormal  Service->System status-> Pressure
 Wipe block blocked
WBC(RBC) clog
 Tube connections between pressure chamber, press
WBC(RBC) bubble ure pump, valves, analog board
Vacuum low  Valve 8,9
 Vacuum pump
Pressure low
 Analog board
Results abnormal
WBC abnormal
Syringe error
Display problem
No start up

Results abnormal
Background
abnormal  Check blood sample
 Check reagents
WBC(RBC) clog
 Check background
WBC(RBC) bubble  Do QC test
Vacuum low  Use control to check CV
 Calibration
Pressure low
Results abnormal
WBC abnormal
Syringe error
Display problem
No start up

WBC abnormal
Background
abnormal  Check lyse
 Lyse test
WBC(RBC) clog
 Lyse not enough, check valve 6 and relevant tube
WBC(RBC) bubble  Lyse enough, then
Vacuum low  Check background
 Check repeatability of WBC, good then calibrate
Pressure low
 Repeatability of WBC not good, check relevant parts
Results abnormal WBC bath, valves 1,3, tubes, analog board
WBC abnormal
Syringe error
Display problem
No start up

Syringe error
Background
abnormal  Service->Self Test-> 7.5mL(50uL) syringe
 Remove tubing connected to syringe, then test again
WBC(RBC) clog
 Add lubricating oil to axis of motor
WBC(RBC) bubble  Syringe sensor dirty or loose
Vacuum low  Disassemble and clean syringe
 Check optical sensor cable, motor cable to power driver board
Pressure low
 Optical sensor defective
Results abnormal  Motor defective
WBC abnormal  Power driver board defective (other motor might have error to
o)
Syringe error
Display problem
No start up

Display problem
Background
abnormal  Check and adjust backlight, contrast
 Check cable between LCD screen and CPU board
WBC(RBC) clog
 No backlight? Converter board defective?
WBC(RBC) bubble  Replace the cable
Vacuum low  Replace LCD screen
 Replace CPU board
Pressure low
Results abnormal
WBC abnormal
Syringe error
Display problem
No start up

No startup
Background
abnormal  Power, fuse
 Disconnect all boards from power supply, use multimeter to me
WBC(RBC) clog
asure output
WBC(RBC) bubble  Only connect CPU board, analog board, if no start, power supp
Vacuum low ly board, CPU board, DOM
 Remove motors, valves, pump from power driver board
Pressure low
 Connect CPU board, power driver board, if no start, power driv
Results abnormal er board defective
WBC abnormal  Short circuits in valves, pumps, motors
Syringe error
Display problem
No start up

2.7 Maintenance

Daily maintenance
If it works day and night or the sample volume is more than 100
per day, execute “E-Z cleanser cleaning” operation once every
day.
If the quantity of samples more than 50pcs, set the “Auto clean
time” 4 hours.
If the user turns off the instrument every day, the user should
execute “probe cleanser cleaning” operation once every three
days. Or do it when the machine remind you.
Use E-Z cleanser to execute shutdown program.
Dispose the waste liquid

Monthly maintenance
 Use probe cleanser to clean the sample probe wipe

block.
 Use sample probe localizer to correct the position of

the sample probe.
 Use E-Z cleanser or probe cleanser to clean the bat

hs.
 Calibrate the unit.

Adjust the sample probe position
Procedure
1. Take off the right side panel of the equipment.
2. Input password, then access the “self test” menu in “service”
3. Press [F1] and select the “mechanics” press [↑]and [] to drive the motor to the upper
place of counting bath.
4. Move up the sample probe to the end
5. Loose the screw that fixed the probe
6. Using localizer to correct the probe and then tighten the screw
Localizer

Half-year maintenance
 Replace vacuum filters.

 Test all valves’ work status.
 Clean the valves, especially the V8, V9,V10.
 Disassemble and clean the two baths and aperture
s.
 Check the stability of HGB blank voltage.
 Check the stability of vacuum.
 Clean inside of the equipment.

Version Information
Version Author Date Description

1.0 Rany 7/11/2012 First Version
2.0 Rany 2/10/2014 N/A

Check Information
Version Reviewer Date Comments

1.0 Carl 7/13/2012 N/A
2.0 Mars Zhang 7/10/2014 Approved

Thank you!

BC-2800 Service Training: Hematology Global Technical Support Department Version V2.0 NO.:MXQ-12028-BC-2800

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BC-2800 Service Training: Hematology Global Technical Support Department Version V2.0 NO.:MXQ-12028-BC-2800

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BC-2800 Service Training

Hematology Global Technical Support Department

1.1 Basic Knowledge

1.1 Basic Knowledge

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 Blood sample consists of two parts.

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 There are three kinds of blood cells.

Leukocyte Erythrocyte Platelet

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Lymph Baso Mono Eosino

Lymphocyte Mid-sized Cell Neutrophilic Granulocyte

 The function of RBC is almost completed by hemoglobin (HGB).

Transport oxygen and carbon dioxide

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2 、 blood cell of Same size, go through the

3 、 blood cell of different size, go through

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 From Pulse Graph to Histogram

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From Pulse Graph to Histogram

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 This analyzer uses a volumetric metering unit to control the count cy

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 HGB is determined by the colorimetric method.

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Absorbing Detector Signal

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8.4” color LCD

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Printer or upgrade interface

Power of floppy disk driver

Environment Space Power

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Environment Space Power

 At least 40 cm on each side, which is the preferred

 At least 10 cm behind for cabling and ventilation

 There is enough room on or below the countertop to

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Environment Space Power

 Check the availability of a power outlet that is

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1. Remove the foam

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1.Open the door of the recorder.

Note: The thermo-side should faces up.

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Start the machine

Test fresh blood sample

Any step has problem,

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Error Messages Reagent Status

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