KTA system Training Guide

Explorer 100 / 10

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KTAexplorer

KTAexplorer
Reverse Flow Valve
(inside)
Std on KTAexplorer 100 only

Injector

Buffers

Box

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Column
Valves

Monitor
Conductivity
pH

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Outlet
Valve

UV Detector
3 Wavelengths
amersham bioscience

Pumps
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KTAexplorer

Pump A

pH/cell
electrode

Buffer switching
valve

Sample
Valve

Sample
holder

KTA system Training Guide

Purifier 100 / 10

sham
amer

amers

ience
biosc

ham bio

scienc

amersham bioscience

am
ersh

am

bios
cien
ce

KTAexplorer 100
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45

Mixer
System
pumps

Reverse
flow valve (V7)

Column
selection
valve (V2)

UV

3 Wavelength
detector

Cond

Conductivity
monitor

pH

pH detector

1 87
2
6
345

Sample
pump

Buffer
select
valve (V6)

1 7
6
2
3 45

Fraction
collector
Frac-920

1 87
2
6
345
1 87
2
6
345

1 87
2
6
345

Outlet
valve (V4)

1 87
2
6
345

Sample selection
valve (V5)

Column
selection
valve (V3)

In-line
filter
Injection
valve (V1)
1 7
6
2
3 45

Mixer
System
pumps

1 7
6
2
3 45

Reverse
flow valve (V7)

Column
selection
valve (V2)

UV

3 Wavelength
detector

Cond

Conductivity
monitor

pH

Sample
pump

pH detector

1 87
2
6
345

Fraction
collector
Frac-950

1 87
2
6
345
1 87
2
6
345

Buffer
select
valve (V6)

1 87
2
6
345

1 87
2
6
345

Air
sensor

Sample selection
valve (V5)

Column
selection
valve (V3)

Outlet
valve (V4)

KTApurifier 10
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45

Mixer

Column
selection
valve (V2)

System
pumps

UV

3 Wavelength
detector

Cond

Conductivity
monitor

pH detector

1 87
2
6
345

Fraction
collector
Frac-950

Buffer
select
valve (V6)

1 87
2
6
345

2
3

7
6
5

Outlet
valve (V4)

1 87
2
6
345

Column
selection
valve (V3)

KTApurifier 10 S
In-line
filter
Injection
valve (V1)
1 7
6
2
3 45

Mixer
System
pumps

1 87
2
6
345

Sample
pump

1 87
2
6
345

Column
selection
valve (V2)

Fraction
collector
Frac-950

1 87
2
6
345
1 87
2
6
345

Buffer
select
valve (V6)

1 87
2
6
345

Sample selection
valve (V5)

Column
selection
valve (V3)

Outlet
valve (V4)

PUMP P-900 Inlets

Mixer

Pump B

Pump A

Buffer Select
Valve IV-908

2
3

A11

A12

A13

1 8
7
6
4

A18

A16

A14
A15

A17

PUMP P-900 Inlets

Mixer

Pump B

Pump A

Switching
Valves
Buffer Select
Valve IV-908

A2

A1

2
3

A11

A12

A13

B1

1 8

B2

Water

7
6
4

A18

A16

A14
A15

A17

Salt

PUMP P-900 Manually Priming Buffer Inlet Lines

Pump A

Common inlet

A2

Pump B

A1

Common inlet

Manual

Flowpath

Manual

A1 or A2

BufferValveA B1 or B2

B1

B2

Flowpath

BufferValveB

Open

Draw A
ir
out of
Buffer
Line

PUMP P-900 Back - Wash Lines

Pump Back-Wash
Lines

Syringe fitting
Wash OUT
IN-LINE
one-way
valve

Buffer
OUT
Ball outlet
check valve

High pressure
Piston seal
Piston

20% Ethanol : Water

Backwash pump
diaphragm

Ball inlet
check valve

Wash
IN

Buffer
IN

ON-LINE FILTERS
P-903 ( 10 ml/min Pump )
Low Flow HPLC Filter

Polymer Support
P-901 ( 100 ml/min Pump )
High Flow Fibre Filter
LOAD (sample)

INJECT (sample)

Waste

3
4

Sample

8
7

PV-908
1

8
7

7
2

Sample

PV-908

PV-908
8

Pump

7
3

6
5

Pump

Pump

Column

Column

Column

LOAD (wash loop)

Waste

Sample

Waste

INJECTION VALVE INV- 907

1
COLUMN
7

PUMP

LOAD
SAMPLE

Waste

Waste

LOAD
1

COLUMN

PUMP

3
6
4

Waste

Waste

INJECT
1
COLUMN

7
2

PUMP
3
4

Waste

6
5

Waste

WASTE

SAMPLE LOADING METHOD

1

COLUMN
2

LOAD
SAMPLE

PUMP

3
6
5

4
Needle Port

Waste

Waste

Always tighten by hand

with needle inserted

Do Not Overtighten

COLUMN
LOAD
SAMPLE

PUMP

3
6
4

Luer Lock
Adapter

Waste

Waste

Superloop

SUPERLOOPS

10 ml
50 ml
Buffer

150 ml

Plunger

BUFFER
FLUSH

SAMPLE
LOAD

SAMPLE
INJECT

SAMPLE
FLUSH

WASTE

PUMP or
SYRINGE

COLUMN

COLUMN

Sample
Etched
Groove

Volume

Max. allowed column

pressure

Code No.

10 ml
50 ml
150 ml

4 Mpa
4 Mpa
2 Mpa

18-1113-81
18-1113-82
18-1023-85

COLUMN SELECTION VALVES (PV-908 )

Pump

MONITOR

REVERSE FLOW VALVE ( INV-907 )

1
7

Pump
6

Column

Extra Tube

Stop
Plug

MONITOR

Downward Flow
Position 1
1
7

Pump
6

3
4

Column

Extra Tube

5
Stop
Plug

MONITOR

Upward Flow
Position 2

COMPRESSION FITTINGS

Untightened

Correctly tightened
Obstructed

Over tightened Tubing Restricted

TM

PEEK Tubing

Gray, 1/16x 1.0 mm ( 7.85ul / cm )

Green, 1/16x 0.75 mm ( 4.42ul / cm )

Orange, 1/16x 0.5 mm ( 1.96ul / cm )

Blue, 1/16x 0.25 mm ( 0.49ul / cm )

Teflon / Tefzel Tubing

3/16 OD

18-1112-47

1/8 OD

18-1121-16

1/16 OD

18-1119-74

System Compensation Volume

Equilibration Phase
A
B

COLUMN TOP
0%

A
B

COLUMN TOP
20%

8 mls
Effect

Command

20%B

0%B

8mls

Gradient Delay Volume

Gradient Phase
A
B

COLUMN TOP
0%

A
B

COLUMN TOP
100%
Effect

16 mls

100%
B

Gradient Delay

Command
100%

0.0MPa
MPa
0.0

Atmos
Atmos
Press
Press

0.0MPa
MPa
0.0

0.0 MPa

Gel Bottom Pressure

(0.3++0.0)
0.0)
(0.3

0.3MPa
MPa
0.3

(0.3- -0.0)
0.0)
(0.3

0.3MPa
MPa
0.3

ColumnPress
PressDrop
Drop
Column

Gel Top Pressure

Pump
Pump
0.3Mpa
Mpa
0.3

0.3Reading
Reading
0.3

0.5MPa
MPa
0.5

(0.3++0.2)
0.2)
(0.3

ColumnPress
PressDrop
Drop
Column

0.2MPa
MPa
0.2
FlowRestrictor
Restrictor
Flow

0.2 MPa

(0.3- -0.2)
0.2)
(0.3

0.1MPa
MPa
0.1

GELPress
PressDrop
Drop
GEL

0.3 MPa

Pump
Pump
0.3Mpa
Mpa
0.3

0.5Reading
Reading
0.5

Matrix Collapses EXPENSIVE

Column Shatters DANGEROUS

Pre-column pressure
= 0 MPa

Column Matrix Pressure Limits

GELPress
PressDrop
Drop
GEL

0.3 MPa

Pre-column pressure
= 0 MPa

Column Pressures

Column Hardware Pressure Limits

Column Pressure ( compensation ) Flow Restrictor