Professional Documents
Culture Documents
Claire Hopkins
Contents
Abstract
Introduction
Echolocation in microchiropteran bats
Call frequency
Call flexibility
Sampling and detection methods
Aims and objectives
4
4
5
5
6
7
8
9
Acoustic survey
Sound analysis
Bat activity
10
10
10
11
12
13
13
Results
Capture data
Species accumulation curves
Capture times
15
15
17
19
19
23
Discussion
25
Future studies
25
26
28
29
30
32
Conclusion
33
Acknowledgements
34
References
35
Abstract
The use of bat detectors in conjunction with traditional capture methods for
creating inventories of microchiropteran communities is becoming increasingly
widespread. However the extent to which bats can be distinguished by the
properties of their echolocation calls is still in dispute. The current study
compares two techniques for detecting bats and evaluates their effectiveness
in contributing toward species lists and elucidating activity patterns in a
previously unstudied area.
Mist nets were deployed at a number of sites in three locations in the
Merendon Mountains of northern Honduras (Base Camp, Buenos Aires and El
Paraiso). A total of 266 bats of 28 species were captured over the 6-week
study period and successfully recorded calls formed the basis of a reference
library. An acoustic survey based on the number of bat passes detected with
a Tranquility detector along a 250m transect was also carried out.
Interspecific variability in sonar properties was found to be low in relation to
intraspecific variation and no statistical differences were found between call
parameters of bats representing similar guilds. While this negates the
reliability of acoustic methods to carry out accurate biodiversity assessments
it highlights potential for recognition of bats according to their foraging guild.
Mist netting remains the most reliable way of identifying bats in the field but
tends to be biased toward Phyllostomid bats foraging in the understorey.
Acoustic monitoring is found to be a convenient method of assessing bat
activity in an area but is sensitive to small scale variations in bat abundance,
foraging patterns and habitat configurations. Future research should aim to
supplement the call reference library with bats from different guilds and further
elucidate ways of recognizing bats acoustically. Patterns of bat activity should
also be established in order to maximize the effectiveness of surveys carried
out in limited time periods.
Introduction
Bats are one of the major taxonomic groups in Honduras comprising
the most species-rich and ecologically diverse mammalian taxon at the local
community level in the Neotropics (Patterson et al. 2001, Kalko 1995). The
ecological importance of bats in tropical forest ecosystems as seed dispersal
and pollination agents and their contribution to the diversity of vertebrate
communities is becoming increasingly recognized. Around 98 species of
microchiropteran bats are currently recognised in Honduras (IUCN, 1994).
This is dominated by the family Phyllostomidae (New World leaf-nosed bats)
which represents a diverse radiation that is endemic to the Neotropics (Kalko
& Handley 2001). Patterns of diversity and abundance in local bat
communities reflect differences in ecological conditions such as levels of
disturbance (Medellin et al. 2000) and the availability of roost sites and
foraging habitats (Wunder & Carey 1996). Attempting to understand the
factors which underlie such patterns has presented important practical
problems for conservation. As such Honduras has been identified as an area
of priority for the investigation of rainforest mammalian diversity in the
Neotropical region (Voss and Emmons 1996). This is a reflection of the
current paucity of data collected in the area and the increasingly fragmentary
nature of prime habitats.
Call frequency
Ultrasonic orientation sounds may be either broad- or narrowband.
Broadband signals cover a range of frequencies while narrowband signals
focus most energy into a smaller range of frequencies (Fenton et al. 1995).
Sounds emitted should have a similar wavelength to the dimensions of the
target object in order to give information about target range, direction, size,
texture and velocity (Altringham 1996). Calls therefore vary widely according
to the species and the interests of the bat in frequency composition and
amplitude, and may contain frequency modulated (FM) components or
constant frequency (CF) components. Patterns of frequency-time structure
based on CF and FM components have been described for some
microchiropteran species. For example, Phyllostomus spp. use multipleharmonic sounds with relatively broad FM sweeps and a large overall
bandwidth which gives high resolution information about targets in complex
habitats (Simmons & Stein 1980). The Mormoopid Pteronotus davyi produces
high-intensity sounds with short CF component at around 68kHz, a downward
FM sweep and short terminal CF component at around 58kHz (OFarrell &
Miller 1997).
behaviour
and
echolocation
call
design
as
measured
by
Call Flexibility
A degree of intra- and inter-individual variety has been demonstrated
by studies of individual bats over extended time periods (Obrist 1995). At least
some of this variation could be explained by genetic (morphometric) variety,
differences between populations and learning (Obrist 1995). Differences
between sex and age also exist (Jones et al. 1992). These call differences
serve several functions in the transmission of information. Unique calls have
the benefit of ensuring self-recognition in the presence of conspecifics and
reduced ambiguity when communicating information about the surroundings
to oneself. Plasticity in the physical properties of calls enabling adjustment to
the environment allows access to a greater variety of habitats. This has been
demonstrated in Pipistrelle bats which adjust their calls to avoid overlap
between echoes from potential prey and obstacles in different vegetation
densities (Kalko & Schnitzler 1993). Studies of the nature of call changeability
have been extended to the Neotropical insectivorous bat Myotis nigricans
7
(Kalko & Schnitzler 2001, OFarrell & Miller 1999) but the full extent of
plasticity in non-insectivorous bats including many members of the
Phyllostomidae has not been investigated in detail. This identifies a lack of
knowledge about the extent to which this group can be used as candidates for
acoustic surveys, given that Phyllostomid bats dominate Neotropical
communities (Read 2002, Kalko & Handley 2001).
Inter-specific variation in call characteristics holds the information
necessary for understanding differences between species (Simmons & Stein
1980). While variation in the frequency-time structure (e.g. frequency
bandwidth, call duration) of insectivorous bats has been used as a means of
species identification in the field (Fenton & Bell 1981, Vaughan et al. 1997,
Rydell et al. 2002), assessments of intraspecific variation with respect to
habitat and behaviour have also been made (Obrist 1995). As Phyllostomid
bats have been observed to have calls which are less variable interspecifically (Kalko 1995) and more difficult to detect and record in the field
(OFarrell & Miller 1997) research is needed to find out the extent to which
different species can be recognised and distinguished by their calls. This is a
fundamental aspect governing the reliability of acoustic surveys (Barclay
1999).
Site selection
Four sites were chosen in the vicinity of each location. Sites selected
represented a range of different habitats, elevations and levels of disturbance
representative of the region, including forest trails, habitat edges and other
areas likely to have concentrated bat activity (Crome & Richards 1988).
Riparian habitats were also sampled as bats frequently use rivers as travel
corridors (Brigham et al 1992). Sites were not selected on the basis of known
10
Capture survey
Following standard protocol, four 5-shelf mist nets of two different lengths (2 x
9m, 2 x 6m) were deployed at each site (e.g. Kunz & Kurta, 1988). The
orientation of nets was influenced directly by the physical characteristics of
the sampling location although where possible nets were places perpendicular
to flyways in such a way as to maximize the chances of capturing bats.
Additional disturbance to the local vegetation was kept to a minimum.
Sampling effort was standardized in terms of number of capture hours
(between 19.30 and 00.00 - coinciding with sunset at this time of year),
frequency of checking (once every 5 10 minutes) and total net length.
Captured bats were identified to species level using a key (M. B. Fenton, pers.
comm.) and field guide (Read 2002). Sex and reproductive condition were
recorded as Jones et al. (1992) have demonstrated call differences between
age and sex groups. Bats were subjected to wing biopsy as a means of
marking captured bats and also biometric analyses including forearm and
mass measurements were performed, as is standard for confirmation of
species identity. Attempts were made to minimize stress to the bats and
heavily pregnant females or highly stressed individuals were released without
analysis.
Species accumulation curves were obtained by taking the number of survey
nights as sampling effort and calculating the cumulative number of species
captured with capture effort. The order in which samples were included in a
species accumulation curve influence its overall shape so sample nights were
randomised to smooth the curve (Magurran 2004). Estimates of overall
species richness at each of the three sites were made using Chaos
11
Diversity
and
Richness
software
(http://www.pisces-
Acoustic survey
A 250m linear transect method was employed to sample free-flying bats of
unknown identity around each site. Calls were recorded using a time
expansion bat detector set to record for 40 ms intervals when triggered by a
bat call. This allows retention of the spectral content of sound information
(Parsons et al. 2000). The detector was placed at a 45 angle to the ground
pointing along the trail or across the open area so as to maximize the
likelihood of detecting high-quality calls (OFarrell et al. 1999). This was done
for at least five minutes at 25m points along the transect - a distance selected
in order to avoid sampling overlapping foraging grounds. Acoustic transect
surveys commenced around 90 minutes after sunset (approx. 19.30) to
ensure constant light intensities across sampling nights and to coincide with
maximum foraging activity (Aldridge & Rautenbach 1987). Using this method
allows bat activity to be sampled regardless of the purpose of the flight (i.e.
foraging or commuting between foraging grounds or roost sites) and sampling
for at least 1 hour each night gives sufficient data to make comparisons
between sites. Recording ceased during periods of rain to minimize risk of
microphone damage and was not carried out within 20m of nets to avoid
detection of any trapped bats. Start points for the transect were rotated on the
second capture night at a site to avoid bias and to take into account
12
differential habitat use by bats. Transects typically followed the forest trails or
natural vegetation breaks as these have been shown to be used by forestdwelling bats (reviewed in Wunder & Carey 1996, Brigham et al 1997, Grindal
1995, Crome & Richards 1988). Transects incorporated parts of the trail
before, around and beyond the netted area in order to sample a comparable
area and acoustic sampling was carried out simultaneously with capture.
Sound analysis
Recorded calls (defined as individual, discrete pulses of sound; OFarrell et al.
1999) were processed using Batsound Pro software on a desktop PC at a
sampling rate of 44100Hz and a Hanning window. Bandwidth, maximum and
minimum frequency (kHz), frequency of maximum energy (frequency at which
the intensity was greatest in kHz) and duration (time in milliseconds from the
beginning to the end of the pulse) were identified in the fundamental call
where possible (Figure 1). Qualitative observations of sonagram shape and
the presence of harmonics were also made. Inter-pulse interval and duty cycle
were not calculated due to the fragmentary nature of calls. Call intensity
measurements were also omitted as this is very sensitive to the direction
traveled and the distance of the bat from the microphone.
Bat activity
I used an index of activity (IA) taken from acoustic monitoring data as a
measure of activity levels in bats (Hayes 1997). On nights when bats were
successfully recorded using the transect method in BA and EP sites the total
number of passes was calculated for each night sampled, where a pass is
defined as a single change of track on the minidisk recorder. Because
recording was limited to 1 disk per night (11 x 5-minute intervals) over
standardized recording hours this method was used to compare activity levels
on a night to night basis. The acoustic IA was compared with a similar IA
obtained from capture data, where activity was equal to the number of bats
caught in the time period covered by the acoustic technique. The acoustic IA
is expected to vary in direct proportion with the IA from captures if the two
methods are sampling similar assemblages of bats such that x number of bat
passes would correspond with y number of captures.
13
Figure 1: Hanning windows showing call spectrograms recorded from bats captured during the
study period. Power spectra are included showing how energy is distributed across frequencies
and harmonics. (A) Sturnira lilium; (B) Artibeus jamaicensis; (C) Molossus sinoloae; (D)
Rhogeessa turmida. (E) and (F) represent two call sequences emitted by Glossophaga soricina.
14
Results
Capture Data
A combined total of 266 bats were captured on 29 survey nights over the 6week study period (Table 1). Representatives from 28 species (plus 4 which
we could not identify), 20 genera and four families were captured in mist nets
at 17 sites over three study locations (Table 2).
Family
Phyllostomidae
Vespertilionidae
Species
BC
Anoura geoffroyi
Artibeus intermedius
Artibeus jamaicensis
Artibeus lituratus
Artibeus phaeotis
Artibeus toltecus
Carollia brevicauda
Carollia perspicillata
Chiroderma salvini
Centurio senex
Desmodus rotundus
Glossophaga soricina
Hylonycteris underwoodi
Micronycteris microtis
Phyllostomus discolor
Phyllostomus hastatus
Platyrrhina helleri
Sturnira lilium
Sturnira ludovici
Tonatia saurophila
Tonatia sylvicolor
Uroderma bilobatum
Vampyrodes caraccioli
Vampyressa pusilla
Eptesicus brasiliensis
Myotis keaysi
Noctilionidae
Noctilio leperinus
Mormoopidae
Pteronotus davyi
9
3
1
2
1
1
1
8
Location
BA
1
7
18
11
2
1
2
5
5
16
8
EP
2
14
34
3
1
11
13
9
17
7
1
2
21
5
1
1
3
1
3
5
2
2
1
1
Unknown
1
34
Total
1
79
2
153
Table 1 Numbers of each species captured in mist nets in the three survey locations (BC =
Base Camp, BA = Buenos Aires, EP = El Paraiso). Captured bats were predominantly from
the family Phyllostomidae although the most abundant species varied between locations.
15
Location
Base
Camp
Buenos
Aires
El Paraiso
Vegetation type
Riparian habitat
Secondary cloudforest
(broadleaved trees)
Secondary forest (pine)
Plantation (banana)
Plantation (banana, coffee)
Landscape mosaic
(plantation/regenerating
forest)
Secondary lowland
rainforest
Plantation (banana)
Ornamental garden near
beach
Man-made clearing
Survey
night
Number of
sampling
sites
1
4
2
6
Combined
acoustic/
capture
0
0
1, 2, 5, 8
3, 4, 6, 7
1
1
2
2
1
1
4
4
0
0
4
4
2, 3, 5, 8
1, 6
2
1
2
2
0
2
4, 7
Table 2: Capture effort for the Neotropical bat inventory. Mist nets were deployed at all 17
sites representing capture and call library data; combined acoustic monitoring and
capture/call library data were obtained from 2 non-consecutive survey nights (numbered) at
each of 4 sites in EP and BA.
Some trap sites were more successful than others and there was
considerable heterogeneity in relative species abundance between sites and
locations (Figure 3). There were distinct between-night effects evident from
variability in both species composition and total number trapped on the 1st and
2nd nights at a site. The limited number of climate recordings taken limits
further analysis of distribution patterns. (Mills et al 1996).
6
5
4
3
2
1
0
-1
BC
BA
EP
Location
Figure 2: Mean capture rates (Bats per hour standard deviation) were generally
high at the lower altitude sites (BA 2.25 1.179; EP 3.40 2.047) and relatively low at
Base Camp sites (0.76 0.932).
16
El Paraiso was the most successful location in terms of both numbers of bats
and numbers of species captured. Mean capture rates were higher at lower
elevations (Figure 2) although high deviation in capture rates at Base Camp
reflect disproportionately high numbers on a night when sampling a riparian
habitat. In addition mean capture rates were significantly larger on the 8
combined acoustic/capture study nights in EP compared with BA (Mann
Whitney U test: z = -2.107, p = 0.035, n = 16). Due to low capture rates and
unfavourable weather conditions during survey nights at all Base Camp sites
and three El Paraiso sites (10 survey nights in total), combined
acoustic/capture data from these sites have been omitted from analyses
although echolocation calls collected from bats captured on these nights were
represented in the call library to demonstrate more of the variation that
occurs.
17
BC
12
10
8
6
4
2
0
0
10
12
Randomised sample
BA
16
14
12
10
8
6
4
2
0
0
10
Randomised sample
EP
25
20
15
10
5
0
0
10
12
Randomised sample
Figure 3 Species accumulation curves for Base Camp (BC), Buenos Aires (BA) and El
Paraiso (EP). Blue diamonds represent average cumulative species number against sample
effort where cumulative species numbers represent average numbers taken from survey data
randomly rearranged according to the number of survey nights in that location (BA = 10, BA =
8, EP = 11). Total species numbers differed nonsignificantly between sites (BC = 11, BA = 14,
EP = 21: 2 =3.435, df = 2, p = 0.1795).
18
Capture time
Capture frequencies of all species for each combined acoustic/capture study
night at BA and EP were grouped into 30-minute time intervals and tested for
differences between sites. At Buenos Aires sites there were no significant
differences between capture nights in timing of captures so data from different
nights were pooled together (2 = 66.827, p = 0.153, df = 56, n = 8). A second
2 analysis was carried out to test the hypothesis that there is no temporal
variation in capture numbers, where expected values are represented by the
total caught over all sites divided by the number of 30-minute time intervals
(9). This test yielded a non significant result suggesting that over the 4 hour
sample period at this time of year and at these sites in BA bat activity appears
to be relatively evenly spread over the night (2 = 9.778, p = 0.281, df = 8).
At El Paraiso there was significant variation in capture times between nights
(2 = 91.589, p = 0.002, df = 56). Capture rates at each site were therefore
analysed on a night by night basis to elucidate patterns of activity. Five out of
8 nights had significantly more captures early on in the evening (between
19:30 and 21:00) than would be expected if activity was uniformly distributed
over the night (df = 8: EP2: 2 = 17.0, p =0.030; EP3: 2 = 16.8, p = 0.032;
EP4: 2 = 38.6, p <0.05; EP7: 2 = 19.368, p = 0.013; EP8: 2 = 22, p =
0.005). However there was a non significant difference between the frequency
of 30 minute intervals with no captures made over time for pooled data
suggesting multimodal patterns of activity (BA: 2 = 7.931, df = 8, p = 0.440;
EP: 2 = 13.33, df = 8, p = 0.101).
19
bats captured at the same sites on different nights by another bat team. This
represented 16 individuals from 6 species making a combined total of 221
calls for analysis.
In order to make comparisons between species call characteristics only those
calls made by individuals of species with more than one individual
representing it were used.
Five parameters and aspects of call structure were measured in the
fundamental component per call. Examination of the echolocation call library
identified considerable variation in call design (Figures 1 & 4).
Figure 4 Typical call structures of
bats captured during the study
period that were represented in
the call library. (a) Steep
broadband FM sweep, often with
harmonics overlapping in
frequency. (b) A combination of
steep and shallow FM sweeps
with initial CF component. (c)
Short FM sweep over small
frequency range, often with
distinct harmonics. (d) Sharp
broadband downward FM sweep,
most energy at the base of the
steepest section. (e) Initial drop in
frequency followed by brief CF
component and steep FM sweep.
(f) Initially shallow followed by
steep FM sweep.
Species
A.intermedius
A.lituratus
A.jamaicensis
A.toltecus
C.brevicauda
C.perspicillata
E.brasiliensis
G.soricina
M.keaysi
M.sinoloae
P.davyi
P.discolor
R.turmida
S.lilium
S.ludovici
a
X
X
X
X
X
X
X
X
Call shape
c
d
e
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
20
21
(A)
90
80
70
60
50
40
30
20
10
0
Frequency of maximum
Energy kHz
(B)
20
40
60
80
100
120
100
90
80
70
60
50
40
30
20
10
0
0
20
40
100
80
60
40
20
0
20
40
60
80
100
2.5
2
1.5
1
0.5
0
-4
-2
80
100
PCA2
Frequency of maximum
Energy kHz
(C)
60
-0.5 0
-1
-1.5
-2
PCA1
A. intermedius
A. lituratus
A. jamaicensis
A. toltecus
C. brevicauda
C. perspicillata
E. brasiliensis
G. soricina
M. keaysi
M. sinoloae
P. davyi
P. discolor
R. turmida
S. lilium
S. ludovici
Figure 6 Principal Components Analysis scores plotted with species markers. Cumulatively
62.706% and 86.466% of the variation observed between species can be explained by PCA1
and PCA2 respectively. Clumping appears to exist in R. turmida and M. sinoloae but most
other species have overlapping values.
22
120
El Paraiso
Date
11/7/04
12/7/04
13/07/04
15/07/04
18/07/04
19/07/04
20/07/04
22/07/04
27/07/04
29/07/04
30/07/04
1/8/04
2/8/04
3/8/04
4/8/04
5/8/04
Site
1
2
3
4
1
4
3
2
1
2
3
4
2
1
4
3
IA
Duration
1:09
1:11
1:11
1:18
1:10
1:08
1:30
1:16
1:14
1:26
1:10
1:22
1:12
1:03
1:33
1:15
Acoustic
8
6
54
26
13
3
88
6
63
9
30
54
24
51
22
8
Capture
3
1
3
2
3
4
2
3
3
2
6
7
9
3
8
13
Total
captures
5
4
17
7
9
8
18
13
10
9
15
10
18
19
19
36
Table 4 Index of Activity (IA) scores for bats detected by acoustic and mist netting
techniques. There were 16 independent sample nights (2 repetitions at each of 4 sites in BA
and EP Table 2). Mean number of bat passes (passes S.E.) were 25.5 10.731 (BA) and
32.625 7.412 (EP).
23
Figure 7 Graphs showing the correlation between Acoustic Index of Activity (IA) and Capture
IA (A) and between Acoustic IA and total captures by location (B). In each case acoustic
indices were not significantly correlated with captures. When data from the two locations were
grouped together acoustic indices were not significantly correlated with either capture IA or
total captures respectively on a night by night basis (Spearmans rank correlation r = -0.034,
p = 0.900; r = 0.385 p = 0.141; n = 16).
A
100
Acoustic AI
80
60
40
Location
20
EP
0
BA
0
10
12
14
Capture AI
B
100
Acoustic AI
80
60
40
Location
20
EP
BA
0
0
10
20
Total Capture
24
30
40
Discussion
Interspecific echolocation call properties of Neotropical bats
In the current study bats were not found to be readily distinguishable on the
basis of their echolocation call structure or design. Interspecific differences in
sonar properties were seen to be small given the amount of variation
observed in conspecifics and in addition bats from different species were
observed to use similar call designs. This contrasts with the limited number of
previous studies investigating echolocation call characteristics of foraging
Neotropical bats, which have found calls to be distinguishable at least to
genus level (OFarrell & Miller 1997, Rydell et al. 2002). While these findings
confounded attempts to differentiate between species on the basis of their
sonar pulse properties alone, it provided useful information pertaining to the
ecology of the bats that use them.
Comparative field studies have demonstrated that inter- and intraspecific
trends in echolocation behaviour are closely associated with ecological
conditions including habitat type, foraging mode and diet, and in turn with
aspects of maneuverability and morphology (Aldridge & Rautenbach 1987,
Crome & Richards 1988, Schnitzler & Kalko 1998). In particular members of
the same feeding guild tend to have evolved similar calls as signal structure
and pattern correspond closely with the environment in which they hunt
(Denzinger et al. 2004). Captures revealed a prevalence of gleaning
Phyllostomid bats and especially frugivores which have been shown to have a
preference for feeding in the understorey (Fleming 1982). Adopting the guild
classification concept developed by Kalko and Handley (2001), the species
represented in the call library upon which analyses were based were
dominated by those suited to highly cluttered space which reflects the bias in
our sampling areas towards forest trails and cluttered habitats.
Sonar signals were dominated by short duration signals typically between 2
and 5 ms long. As emitted pulses greater than 5.9ms long will cause overlap
between pulse and echo from objects 1m away, short pulses avoid this
overlap and are well suited to bats foraging in close proximity to obstacles
(Altringham 1996). Steep broadband sweeps of mid-frequency range, often
supplemented with harmonics that overlap (Figure 1), give large overall
bandwidth and sharp target ranging acuity. Such calls have been
25
different species of free flying bats on call libraries obtained from bats
adjusting to atypical situations. Variation within and among populations and
variation according to recording differences can augment the problems of
using echolocation calls to identify bats (Barclay 1999). Reliable identification
of bats could at this stage only be increased by supplementing call data with
other morphometric data including size and shape of the bat which are difficult
to obtain without capture. Rydell et al. (2002) found that open situations give
more typical calls as it is least difficult for bats to obtain supplementary
information visually, and it may be possible to standardize call recording
methods by always releasing bats in a similarly open habitat.
It appears that variation within species is larger than can be explained by
variation in survey site alone given that different individuals belonging to the
same species were observed to emit calls of different designs even if
captured at the same site (pers. obs). Small sample size, context, behaviour
and genetic differences should not be discounted as proximal causes of the
observed variation. Low success rates for call recording possibly reflected the
fact that Phyllostomids are whispering bats that emit low intensity sounds
that are rarely registered with detectors (Arita & Fenton 1997, Kalko 1997),
and that are less reliant upon echolocation for navigation and foraging
(Altringham & Fenton 2003). This may also account for poor call quality and
the fragmentary nature of calls recorded.
Duffy et al. (2000) suggested that a library of between 15 and 40 reference
calls would be necessary to represent the variation between individuals in
search-phase calls in bats at each site. On the basis of this study it is
suggested that even more calls would be required to accurately represent the
full range of call variations achievable by each species over the range of
habitats sampled and in order to identify trends according to geographical
distance (OFarrell et al. 2000), sex and reproductive condition (Jones et al.
1992).
The current study has provided a baseline acoustic reference which should be
supplemented by thorough future sampling efforts to provide a representative
sample of the full repertoire of bats.
27
Timing of capture
The non significant difference between capture rates at different times in the
night highlights that caution should be applied when basing acoustic
monitoring studies on specific time periods during a survey night. The
assumption had been made that maximum periods of activity in different bat
species would coincide around 2 hours of sunset when opportunistic feeding
takes place (Aldridge & Rautenbach 1987). Although El Paraiso data show a
general trend toward early activity patterns of bat activity consistent with
Eckerts and Hayes observations (Eckert 1982, Hayes 1997), the pattern was
less obvious in Buenos Aires. There appeared to be persistent overall activity
levels at moderate levels with multiple peaks during the 4.5 hour sampling
period each night and additionally, the distribution of 30-minute periods with
no captures did not differ significantly on a night to night basis. In
concordance with the findings of Crome and Richards (1988) no consistent
differences were observed between species in timing of foraging activity and
Phyllostomid bats showed a tendency to be active over long time periods
(Eckert 1982). Fluctuation in activity patterns has been demonstrated to be
influenced strongly by prevailing external and physiological conditions (Eckert
1982). Furthermore, a number of other factors were seen to affect timing of
capture. There appeared to be less activity on moonlit nights (pers. obs.)
consistent with Morrisons theory of lunarphobia (Morrison 1978). The
influence of foraging strategy and conspecifics on flight times and capture
numbers was also observed as some frugivores forage in groups (Fleming
1982) and Artibeus species tended to be trapped in quick succession,
possibly as a result of attraction to the distress calls of conspecifics
(Altringham & Fenton 2003).
On the basis of the implications of this study two trap nights do not appear to
be adequate to obtain reliable estimates of bat species in forest areas (cf.
Mills et al. 1996). A more in-depth understanding of activity patterns in
conjunction with more detailed knowledge of bat composition in the area will
have important implications for the design of acoustic surveys. For the
purposes of carrying out biodiversity assessments whole night sampling is
recommended to detect bats with more elusive foraging patterns.
28
implies that given the number of trophic niches available the number of
species actually represented in the inventory were relatively low. Biodiversity
estimates calculated in this study were based on relative abundance although
as has been discussed, absence from a site at a particular time and using a
particular detection method does not categorically define it as being absent.
Differences in flight frequency, variation in habitat structure, differences in
vertical movements and the proportion of time spent in the sampling zone will
all have an effect on captures. It should not be assumed that such influences
have no significant effect on the number and relative proportions of bat
species bats captured at a site (Remsen & Good 1996).
The failure of the acoustic reference library to demonstrate means of
differentiating between species based on echolocation calls meant that
ultrasound surveys could not be used to complement mist netting data in
compilation of species lists (cf. OFarrell & Miller 1997, OFarrell & Gannon
1999). Establishing a bigger call library and overcoming the biases inherent
with mist netting should be priorities for the design of future biodiversity
assessments. In future it may be prudent to limit biodiversity measures to
families of bats belonging to common and ubiquitous taxonomic and
biogeographic units, and especially to polytypic genera such as Glossophaga,
Sturnira and Artibeus spp. which can be sampled using standardised protocol
(Moreno & Halffter 2000). This will allow data between inventories to be
directly compared and, importantly, for analysis of biodiversity in relation to
community structure and ecosystem modification.
Analysis of species composition at the landscape level can be useful for
detecting and evaluating the effects of habitat change for example as a result
of anthropogenic activities, and for comparing the biodiversity in different
geographical areas, communities or guilds (Moreno & Halffter 2000, Medellin
et al. 2000). The level of completeness of species lists therefore has the
potential to influence studies of diversity, macroecology and conservation by
providing a predictive tool.
then
call
and
capture
techniques
could
yet
provide
31
Accurate and precise estimates of activity levels derived using bat detectors
will be obtainable only with intensive sampling effort (Hayes 1997) and
ultrasonic monitoring studies should be designed to minimise the effects of
high variability in bat activity at a site among nights.
Future studies
This study was a pilot which may form the basis of forthcoming regional
surveys of microchiropteran bats. The study has revealed that not all species
and not all individuals of a species are equally susceptible to different forms of
detection. The reasons for this are not known although they are thought to be
due to differential use of space and vocalization (OFarrell & Gannon 1999).
Bat guilds can comprise many different species that have each evolved
differently in order to catch specific prey, but forest interior guilds of bats in
Honduras appear not to be partitioned according to echolocation behaviour or
timing of foraging using the parameters measured. This contrasts with Heller
& von Helversons 1987 study, which demonstrated resource partitioning
according to sonar frequency bands in Rhinolophid bats. Further investigation
into the correlates of species distribution and resource partitioning should aim
to investigate the alternative parameters of echolocation call design including
pulse interval, duty cycle and the properties of harmonics (Fenton et al 1998)
as well as prey specificity and morphology and the means by which bats in
the Neotropics partition resources.
Future studies monitoring bat activity and habitat use should concentrate on
identifying ways of boosting capture rates for example by using harp traps
which are better suited to forest trails and flyways, as variation in capture
numbers may have been due to the structural characteristics of flyways
influencing the trappability of bats (Kunz & Kurta 1988). Use of canopy nets
will also increase the completeness of biodiversity surveys by sampling
different guilds (Bernard 2001, Wunder & Carey 1996, Kalko & Handley
2001).
32
Conclusion
Neotropical bats captured in mist nets in the understorey were found to have
indistinguishable sonar characteristics which may be attributable to the
similarities in their foraging habitat. Not all bat species are equally susceptible
to different forms of detection and this was reflected by the absence of
correlation between activity levels monitored using mist nets and bat
detectors. This study has demonstrated that mist netting is currently the most
reliable and accurate way of creating inventories of bats, especially given the
difficulties with standardization of acoustic methods in the field. However mist
netting is seen to under represent particular guilds of bats. Supplementation
of call reference libraries with calls from more individuals and species is
expected to increase the resolution at which correct species identification can
be made and will allow quicker and more representative biodiversity
assessments to be produced.
33
Acknowledgements
Id like to thank all of the members of team bat who worked with me in
Honduras: John Altringham, Paula Senior, Sally Griffiths, Tory Bennett, Zoe
Davies and Oscar Arostegui. I would also like to thank Operation Wallacea for
providing the logistical arrangements necessary for carrying out the project,
COHDEFOR for the permits and to Roberto Downing for his help and advice.
34
References
Aldridge, H. D. & Rautenbach, I. L. (1987). Morphology, echolocation and
resource partitioning in insectivorous bats. Journal of Animal Ecology 56, 763
778.
Altringham, J. D. (1996). Bats Biology and Behaviour. OUP, Oxford.
Altringham, J. D. & Fenton, M. B. (2003). Sensory Ecology and
communication in Chiroptera. In Bat Ecology (Kunz, T. H. & Fenton, M. B.
eds). University of Chicago Press, Chicago.
Barclay, R. M. (1999). Bats are not birds a cautionary note on using
echolocation calls to identify bats a comment. Journal of Mammalogy 80,
290 296.
Belwood, J. J. (1988). Foraging behaviour, prey selection and echolocation in
phyllostomine bats (Phyllostomidae). In Animal Sonar 601 605. (eds
Nachtigall, P. E. & Moore, P. W. B.). Plenum Press, NY.
Bogdanowicz, W.,, Fenton, M. B. & Daleszczyk, K. (1999). The relationships
between echolocation calls, morphology and diet in insectivorous bats.
Journal of Zoology (London) 247, 381 393.
Brigham, R. M., Grindal, S. D., Firman, M. C. & Morissette, J. L. (1997). The
influence of structural clutter on activity patterns of insectivorous bats.
Canadian Journal of Zoology 75, 131 136.
Brigham, R. M., Aldridge, H. D. & Mackey, R. L. (1992). Variation in habitat
use and prey selection by Yuma bats, Myotis yumanensis. Journal of
Mammalogy 73, 640 645.
Bernard, E. (2001). Vertical stratification of bat communities in primary forests
of Central Amazon, Brazil. Journal of Tropical Ecology 17, 115 126.
Colwell, R. K. & Coddington, J. A. (1995). Estimating terrestrial biodiversity
through extrapolation. Philosophical Transactions of the Royal Society of
London ser. B. (Biological Sciences) 345, 101 118.
Dear, S.P., Simmons, J. A., & Fritz, J. (1993). A possible neuronal basis for
representation of acoustic scenes in the auditory cortex of the big brown bat.
Nature 364, 620 623.
Denzinger, A., Kalko, E. K. V. & Jones, G. (2004). Ecological and evolutionary
aspects of echolocation in bats. Pp 311 327 in Echolocation in Bats and
Dolphins, ed. J. A. Thomas, C. F. Moss & M. Vater. University of Chicago
Press, Chicago.
35
Duffy, A. M., Lumsden, L., Caddle, C. R. & Chick, R. (2000). The efficacy of
Anabat ultrasonic detectors and harp traps for surveying microchiropterans in
south-eastern Australia. Acta Chiropterologica 2, 145 153.
Eckert, H. G. (1982). Ecological aspects of bat activity rhythms. Pp. 201 242
in Ecology of Bats, ed. T. H. Kunz. Plenum Press, New York.
Fenton, M. B., Portfors, C. V., Rautenbach, I. L. & Waterman, J. M. (1998).
Compromises: sound frequencies used in echolocation by aerial-feeding bats.
Canadian Journal of Zoology 76, 1174 1182.
Fenton, M. B., Audet, D., Obrist, M. K. & Rydell, J. (1995). Signal strength,
timing and self-deafening: the evolution of echolocation in bats. Paleobiology
21, 229 242.
Fenton, M. B. & Bell, G. P. (1981). Recognition of species of insectivorous
bats by their echolocation calls. Journal of Mammalogy 62, 233 243.
Fenton, M. B. (1982). Echolocation, Insect hearing, and feeding ecology of
insectivorous bats. Pp261 285 in Ecology of Bats ed. T. H. Kunz. Plenum
Press, New York.
Fleming, T. H. (1982). Foraging strategies of plant visiting bats. Pp. 287 325
in Ecology of Bats, ed. T. H. Kunz. Plenum Press, New York.
Griffin, D. R. (1971). The importance of atmospheric attenuation for the
echolocation of bats (Chiroptera). Animal Behaviour 19, 55 61.
Grindal, S. D. (1995). Impacts of forest harvesting on habitat use by foraging
bats in southern British Colombia. M.Sc Thesis, University of Regina, Regina,
Sask.
Heller, K-G. & v. Helverson, O. (1989). Resource partitioning of sonar
frequency bands in Rhinolophid bats. Oecologica 80, 178 186.
IUCN Red List of Threatened Species (2003) IUCN World Conservation
Union, Gland, Switzerland. www.redlist.org.
Jones, G., Gordon, T. & Nightingale, J. (1992). Sex and age differences in the
echolocation calls of the lesser horseshoe bat Rhinolophus hipposideros.
Mammalia 56 189 193.
Kalko, E. K. V. (1998). Organisation and diversity of tropical bat communities
through space and time. Zoology 101, 281 297.
Kalko, E. K. V. & Schnitzler, H-U. (1998). The roles of echolocation and
olfaction in two Neotropical fruit-eating bats, Carollia perspicillata and C.
castanea. Behavioural Ecology and Sociobiology 42, 397 409.
36
37
38
39