GREEN TECHNOLOGY FOR 6-AMINOPENICILLANIC ACID PRODUCTION

Ramona Mihaela MATRAN, Dan CACAVAL,

Alexandra Cristina BLAGA
"Gheorghe Asachi" Technical University of Iasi, Faculty of Chemical Engineering and
Environmental Protection, Dept. of Organic, Biochemical and Food Engineering, Iasi, Romania

2. MATERIALS AND METHODS

1. INTRODUCTION

6-Aminopenicillanic acid is the main precursor for semi-synthetic beta-lactamic

antibiotics production (amoxycillin, ampicillin, etc.).
This precursor could be obtained by means of chemical synthesis and also by
Penicillium spp. fermentation without precursor, but the current method applied at
industrial scale for 6-Aminopenicillanic acid production consists on the deacylation of
natural Penicillins.
This work studied the efficiency of enzymatic hydrolysis of Penicillin G to 6Aminopenicillanic acid under substrate inhibition for a bioreactor with mobile bed of
immobilized penicillin amidase
pH [7, 9], t [20 oC, 40 oC]
: (1; 1,5: 2) mm.

3. RESULTS

(t = 30oC, process = 5 h)

70
3

CPG = 80 mol/m
dP = 1.0 mm
dP = 1.5 mm
dP = 2.0 mm

60
50

CPG = 200 mol/m

dP = 1.0 mm
dP = 1.5 mm
dP = 2.0 mm

40
30
7,0

7,5

8,0

8,5

Hydrolysis yield, %

Hydrolysis yield, %

80

14

70
3

CPG = 80 mol/m
dP = 1.0 mm
dP = 1.5 mm
dP = 2.0 mm

60
50
40

9,0

30
20
20

25

30

8
6

45

2,0

65
60

12

C 6 APA
P
t

Particle diameter, mm

Influence of biocatalyst particle size on

hydrolysis yield (pH = 8, t = 30oC, process = 5 h)

1,4

150

1,2
1,0

100

0.37

4. CONCLUSIONS

55

2,0

200

300
250

3
,
mol/m
h
P 1,17 3.2110
3
dP

dP = 1.0 mm
dP = 1.5 mm
dP = 2.0 mm

50

1,6

CPG

15

3
3

1,8

Influence of temperature and biocatalyst particle size

on hydrolysis yield

Productivity, mol/m h

1,5

40

75

1,0

35

T, C

80

CPG = 200 mol/m

10

CPG = 200 mol/m

dP = 1.0 mm
dP = 1.5 mm
dP = 2.0 mm
15

85

70

12

10

Influence of pH-value and biocatalyst particle size

on hydrolysis yield

CPG = 80 mol/m

= 8,process = 5 h)

80

pH-value

Hydrolysis yield , %

(pH

90

100

150

200

250

300

CPG, mol/m

Influence of Penicillin G concentration

on hydrolysis productivity
(pH = 8, t = 30oC)

The enzymatic hydrolysis of Penicillin G to 6-Aminopenicillanic

acid with penicillin amidase immobilized in Eupergit C particles indicated
a optimum evolution of the process at the 8 pH-value and temperature
of 30oC, regardless of particless size.
The inhibitory effect of substrate could be diminished by cells
immobilization, the substrate consumption rate increasing with the
increase of biocatalyst particle size.