Name: _____________________________________________

Genetics - BIOL 2490

25 Feb 2010
Interrupted Conjugation practice problem:
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Using the technique of interrupted mating, four different Hfr strains of E. coli were mated to an
F- strain to determine the origin and the sequence of genes on the bacterial chromosome. Each
of the Hfr strains transmitted its genes to the F- strain in a unique sequence. Construct a circular
map for E. coli placing each gene in its proper order on a circular map and note the time in
minutes between adjacent genes. NOTE: The experimentally useful genes differ among the
strains, but the gene order on the E. coli chromosome does not change.

Order and transfer and

Culture time of transfer in minutes
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A mal - met - thi - thr - tyr
(10) (17) (22) (33) (57)

B arg - thy - met - thr

(15) (21) (32) (48)

C his - phe - arg - mal

(18) (23) (35) (45)

D phe - his - bio - azi - thr - thi

(6) (11) (33) (48) (49) (60)
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