Name: ...................................................................

Period:...................................................................
Date:......................................................................
Lab: 5
Topic: Photosynthesis
Aim: To Demonstrate Carbon Dioxide consumption by Plants.
PI
N

Objective
To be able to use indicators
and accurately read
change based on color

Rubric
4 Mastered: Students will be able to follow instructions to accurately
read change that is an indicator of chemical nature based on pH
3 Proficient: NA

intensities and pH.

2 Developing : NA
1 Beginning : Students can follow instructions to use chemical
indicators but cannot read or identify the indicator.
0 No Basis : Students cannot follow instructions to accurately read
change that is an indicator of chemical nature based on pH

To write an accurate
hypothesis.

4 Mastered: Students can write a hypothesis in a statement form, that is

testable, containing a cause and effect .
3 Proficient: Students can write a hypothesis in a statement form that it
contains a cause or an effect but is too wordy and contains irrelevant
information.
2 Developing : Students write a hypothesis that is in a statement form
that contains does not cause or an effect hence it is not testable but is
related to the topic.
1 Beginning : Students writes a hypothesis in a question form and is
testable and relates to the topic.
0 No Basis : Students cannot write a hypothesis in a statement form,
that is testable or containing a cause or effect and not related to the
topic.

To be able to differentiate
between a dependent and
independent variable.

4 Mastered: Students can identify both the dependent and independent

variable
3 Proficient: Students can identify both the dependent or independent
variable but the variables are too wordy and includes irrelevant
information.
2 Developing : Students can identify either the dependent or
independent variable.

Score

1 Beginning : Students can identify either the dependent or independent

variable but the one identified is too wordy and includes irrelevant
information.
0 No Basis : Students cannot identify either of the variables.

To be able to identify the

control group and controlled
variables.

4 Mastered: Students can identify the control group and all controlled
variables
3 Proficient: Students can identify the control group and a few control
variables
2 Developing : Students can identify the either control group or a
few/all the control variables.
1 Beginning : N/A
0 No Basis : Students cannot identify the control group or any control
variables

V(i)

To be able to use the claim

as the first part of analyzing
data.

4 Mastered: Claim answers the question, is accurate, and is

complete. Completely describes the trend in the relationship
between the IV and DV where appropriate.
3 Proficient: Claim answers the question, and is accurate, but is
incomplete
2 Developing : Claim does answer the question but it is
inaccurate.
1 Beginning : Claim does not answer the question (i.e., describe
the relationship between the 2 variables)
0 No Basis : Does not make a claim

V(ii)

To be able to use evidence

as part of analyzing data.

4 Mastered: Provides appropriate and sufficient evidence to

support the claim
3 Proficient: Provides appropriate and sufficient evidence to
support the claim. May include some inappropriate evidence.
2 Developing : Provides appropriate, but insufficient evidence to
support the claim. May include some inappropriate evidence
1 Beginning : Only provides inappropriate evidence (evidence
does not support the claim)
0 No Basis : Does not provide evidence

V(iii)

To be able to use evidence

as part of analyzing data.

A
Making observations of lifes
processes that is
demonstrated in the
experiment.

4 Mastered:Includes explanation of science concepts that link

evidence to the claim (concepts are appropriate), and they are
sufficient (no omission of key science concepts) and are clearly
stated and accurate
3 Proficient: Includes explanation of some science concepts that
link evidence to the claim, but are insufficient (one or more
concepts that should have been included are not included) or
some are inappropriate
2 Developing : Includes explanation of science concepts but all
are inappropriate concepts that do not link evidence to claim
1 Beginning : Restates evidence and does not include
explanation of science concepts
0 No Basis : Does not include reasoning

Background information:
Photosynthesis is essential for life. Certain organism can make its own food such as green plants, algae,
and certain protist and bacteria. The conversion of light energy to chemical energy is done in the
chloroplast of cell. In these cells there is a pigment called chlorophyll which absorbs light energy.The light
energy is then used to combine water and carbon dioxide to make sugar and oxygen. Bromothymol blue
is a chemical indicator, when acid is added it changes to yellow. This indicator can be used to indicate if
Carbon dioxide is used by plants . If Carbon Dioxide is removed by the process of photosynthesis the
yellow color will change back to blue.

Materials:
2 test tubes
4 Corks
2 Elodea springs
water
marker
Aluminum foil
HCL
Bromothymol Blue
Glass Stirrer

(1) Write a hypothesis; What happens to Carbon Dioxide when light is given to plants?

.....................................................................................................................................................................
(2) If light is given to the experimental sample, how would the control group be treated differently?

.....................................................................................................................................................................
(3) Identify the Dependent and Independent Variable

Dependent Variable:.................................................................

Independent Variable:...............................................................

Procedure/Method
(1) Label the 4 test tubes as follows;

(2) Fill each test tube with about two thirds of water.
(3) In each test tube add 1 drop of bromothymol blue solution into each test tube and mix thoroughly.
(4) Add 1 drop of HCL solution to each tube and mix by thoroughly using a glass stirrer.
(5) Add another drop of HCL solution to each test tube and mix thoroughly, if the color is all yellow move
on to step 6, if its still blue repeat step 5 until the color is yellow.
(6) Take one strand (stem) of Elodea plant each and place it slowly into test tube 1 and 2 to avoid any
liquid overflow.
(7) Cork (cover) each tube and swirl the tubes to mix the contents.
(8) Place tubes 1 in bright light under the lamp for 30 minutes and tubes 2 in dark (Wrap with aluminum
foil so that light does not get to it) for 30 minutes.
(9) Record your result in the table below:

Test Tubes
Elodea with Light

Color change after 30 mins

Elodea without Light

(10) Analyze your results using a DSET

Complete Explanation Paragraph

ENSURE TO WASH AND CLEAN WORK STATION.