TN HC VIN.

CU 1: Trnh by theo bng vi cc chi tit yu cu nh sau

N

Cng thc khai trin ton phn

(khng c vit tt hoc s dng k hiu;
H

hnh thc trnh by thng nht

COOH
NH2

Tn nguyn

Tn
bng 3
k t

Tn
bng
1 k
t

Tnh
cht
phn
cc ?

tnh
acid/
base?

pI

Ba amino acid c tnh trung ho (Khng k glycin)

1

Hai amino acid c tnh acid

1

Bn amino acid c tnh base

1

Hai amino acid c cha nguyn t sulfur

1

Ngi ta phn loi cch vit tn vi 1 k t hoc vi 3 k t vi mc ch g?

CU 2: Cc peptid v protein thin nhin u c to thnh t 21 loi -amino acid, v cc nghin cu cho thy
cc -amino acid u l loi L-amino acid cng l cu hnh S. Tuy nhin, tm thy trong thin nhin mt s
amino acid cu hnh R, th d trong con trng (con giun t) c (R)-serin v trong u trng ca cn trng c (R)alanin.
a/ Da vo qui tc no m c danh php D-amino acid hoc L-amino acid

b/ Vit cng thc Fischer ca (R)-serin v (R)-alanin (Vit ng qui tc cch vit cng thc Fischer)

c/ Hai hp cht nu trn l amino acid D hay L?. Gii thch

d/ Khi o nng lc trin quang th (R)-serin hu trin hay t trin, gii thch r

e/ Khi cho mt hn hp gm (R)-serin v (R)-alanin ln u ct sc k silica gel, hi c th tch ring hai amino
acid ny ra ring c khng. Nu c, ci no ra khi ct trc, phi gii thch c th.

f/ C mt hn hp tiu trin l dn xut amino acid l acid (R/S)-2-amino-4-phenylbutanoic.

Cc nghin cu trc cho bit i phn (S) c hot tnh sinh hc rt hp dn,
Vit cu trc ho lp th ca hai hp cht

ngh phng php tch ring ra c i phn ny.

CU 3: Tr li cc cu hi sau
N
O

OH
C
CH

HOH2C

NH
NH
C

CH

NH
C

O
O

COOH

H2C

O
NH

CH

CH2
CH

H2C

NH
O

NH
CH

CH2

CH2
CH
NH2

H2C

H2N

CH
NH

NH

C
O

CH3

a/ Hp cht trn c th gi l monopeptid, oligopeptid hay polypeptid: .....................................................

Gii thch v sao c gi nh th

Hn na, c th gi c th l g? (dipeptid, tripeptid...?)............................................................................

Gii thch v sao c gi nh th

Ngay trn hnh, s dng nt gch chm chm, s ng

ct cc ni amid v ngay tng phn hy
cho bit l amino acid no. Vi mi n v amino acid hy vit tn gi nguyn v k hiu vit tt bng 3 ch.
Vit li cng thc khai trin ca peptid ny nhng ch trn mt hng v trnh by theo qui c quc t

Gi tn chui peptid ny theo qui c quc t (k hiu amino acid vit tt bng 3 ch).

CU 4/ Trong t nhin, hai mi hai amino acid thng dng c sp xp vo loi amino acid c tnh:
khng phn cc/ phn cc, tnh acid/ tnh base
a/ Da vo iu g m ngi ta phn bit cc tnh cht ca cc amino acid.

b/ Phn bit nh th c ch li g, phi trnh by c th

c/ Th no l gi tr pI ca mt amino acid?

d/ Bit gi tr pI ca mt peptid/protein c th gip ch c iu g